Biodekolorisasi Metil Oranye oleh Bakteri Uncultured Pseudomonas sp. Clone 034571 018 yang Terimobilisasi pada Matriks SA-PVA-Bentonit

Galuhingtyas, Eunike Elveno (2025) Biodekolorisasi Metil Oranye oleh Bakteri Uncultured Pseudomonas sp. Clone 034571 018 yang Terimobilisasi pada Matriks SA-PVA-Bentonit. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Industri tekstil merupakan salah satu penyumbang utama limbah cair yang mengandung zat pewarna sintetis seperti metil oranye (MO), yang bersifat toksik dan sulit terdegradasi secara alami. Penelitian ini bertujuan untuk mengevaluasi kemampuan biodekolorisasi senyawa MO oleh isolat bakteri Uncultured Pseudomonas sp. Clone 034571 018 (JN1) yang terimobilisasi dalam matriks SA-PVA dan SA-PVA-Bentonit tanpa penambahan media nutrient broth (NB) pada MO, sebagai kondisi yang menyerupai lingkungan minim nutrien di lapangan. Beads disintesis dengan perbandingan SA:PVA dan SA:PVA:Bentonit sebesar 1:2 dan 1:2:1 (%b/v), kemudian digunakan dalam proses dekolorisasi MO 10 mL dengan konsentrasi 10 mg/L selama 10 hari dalam kondisi statis pada suhu 37°C. Hasil penelitian menunjukkan bahwa variasi beads SA-PVA-Bentonit-JN1 memberikan hasil paling baik dengan persentase dekolorisasi 42,34%, diikuti oleh SA-PVA-JN1 (32,43%), SA-PVA-Bentonit (25,30%), dan SA-PVA (22,85%). Uji reusabilitas menunjukkan bahwa efektivitas dekolorisasi menurun signifikan pada siklus ketiga. Karakterisasi FTIR dan SEM-EDX memperkuat indikasi terjadinya interaksi antara gugus fungsi penyusun beads dengan molekul MO. Penelitian ini membuktikan bahwa imobilisasi bakteri JN1 dalam matriks SA-PVA-Bentonit efektif untuk aplikasi awal pengolahan limbah pewarna secara biologis dengan kondisi tanpa penambahan media NB, walaupun efektivitasnya masih perlu ditingkatkan, misalnya melalui modifikasi struktur matriks dengan penambahan senyawa penstabil.
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The textile industry is one of the major contributors to wastewaters containing synthetic dyes such as methyl orange (MO), which are toxic and difficult to degrade naturally. This study aimed to evaluate the biodecolorization capability of MO by an bacterium isolate of Uncultured Pseudomonas sp. Clone 034571 018 (JN1) immobilized in SA-PVA and SA-PVA-Bentonite matrices, without the addition of nutrient broth (NB) to the MO solution, simulating low-nutrient environmental conditions. Beads were synthesized using SA:PVA and SA:PVA:Bentonite ratios of 1:2 and 1:2:1 (%w/v), then applied to 10 mL of MO solution at a concentration of 10 mg/L for 10 days under static conditions at 37°C. The results showed that the SA-PVA-Bentonite-JN1 beads variation achieved the highest decolorization percentage of 42,34%, followed by SA-PVA-JN1 (32,43%), SA-PVA-Bentonite (25,30%), and SA-PVA (22,85%). Reusability test indicated a significant decline in decolorization efficiency by the third cycle. FTIR and SEM-EDX characterizations confirmed the interaction between the functional groups of the beads and MO molecules. This study demonstrates that immobilizing JN1 bacteria in SA-PVA-Bentonite matrix is effective for initial biological treatment of dye-containing wastewater under nutrient-deficient conditions, although further enhancement of effectiveness is needed, for example through matrix structure modification with stabilizing compounds.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Metil Oranye, Pseudomonas JN1, Imobilisasi, SA-PVA-Bentonit, Biodecolorization, Methyl Orange, Pseudomonas JN1, Immobilization, SA-PVA-Bentonite
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Eunike Elveno Galuhingtyas
Date Deposited:	05 Aug 2025 00:47
Last Modified:	05 Aug 2025 00:47
URI:	http://repository.its.ac.id/id/eprint/127197

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