Sintesis Nanopartikel Perak (AgNPs) dengan Madu Klanceng (Trigona sp.) sebagai Capping Agent: Optimasi menggunakan BBD-RSM

Tsaniputri, Shofiyah Edliana (2025) Sintesis Nanopartikel Perak (AgNPs) dengan Madu Klanceng (Trigona sp.) sebagai Capping Agent: Optimasi menggunakan BBD-RSM. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Nanopartikel perak (AgNPs) merupakan salah satu nanopartikel yang memiliki potensi aplikasi pada berbagai bidang, salah satunya adalah biomedis. AgNPs merupakan agen antioksidan yang efektif dengan toksisitas rendah. Namun, AgNPs memiliki stabilitas yang rendah sehingga mudah aglomerasi. Oleh karena itu, perlu adanya penambahan capping agent yang dapat berasal dari senyawa metabolit primer maupun sekunder pada bahan alam di Indonesia. Penelitian ini bertujuan untuk sintesis AgNPs dengan capping agent dari madu klanceng. Sintesis AgNPs dilakukan dengan metode reduksi kimia menggunakan AgNO3 sebagai prekursor logam, NaBH4 sebagai reduktor, dan madu klanceng sebagai capping agent. Optimasi sintesis dilakukan dengan metode Box Behnken Design (BBD) - Response Surface Methodology (RSM). Terdapat tiga parameter yang dianalisis, yaitu konsentrasi madu klaceng (5; 10; 15 ppm), waktu sonikasi (30; 60; 90 menit), dan suhu sonikasi (40; 60; 80°C). Karakterisasi AgNPs hasil sintesis dilakukan menggunakan instrumen Spektrofotometer UV-Vis, Particle Size Analyzer (PSA), Transmission Electron Microscopy (TEM), dan Fourier Transform Infra Red (FTIR). Kondisi optimum hasil AgNPs pada penelitian ini adalah waktu sonikasi selama 70 menit dengan suhu sonikasi 40°C dan konsentrasi madu klanceng 10 ppm. Nanopartikel perak yang dihasilkan berupa dispersi berwarna kuning cerah transparan, berbentuk bulat (spherical), memiliki ukuran dengan rata-rata 25,7 nm, dan nilai PDI (polydispersity index) sebesar 0,2738. AgNPs hasil optimasi dilakukan pengujian aktivitas antioksidan menggunakan DPPH (2,2-difenil-1-pikrilhidrazil). Aktivitas antioksidan dari AgNPs hasil optimasi memiliki nilai IC50 sebesar 29,56 ppm yang berarti merupakan penghambat radikal bebas DPPH sangat kuat.
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Silver nanoparticles (AgNPs) are one type of nanoparticle with potential applications in various fields, including biomedicine. AgNPs are effective antioxidants with low toxicity. However, AgNPs have low stability and tend to agglomerate. Therefore, it is necessary to add a capping agent that can be derived from primary or secondary metabolites in natural materials found in Indonesia. This study aims to synthesize AgNPs with a capping agent from klanceng honey. The synthesis of AgNPs was carried out using a chemical reduction method with AgNO3 as the metal precursor, NaBH4 as the reductor, and klaceng honey as the capping agent. The synthesis optimization was performed using the Box Behnken Design (BBD) - Response Surface Methodology (RSM). Three parameters were analyzed, namely the concentration of klanceng honey (5; 10; 15 ppm), sonication time (30; 60; 90 minutes), and sonication temperature (40; 60; 80°C). The characterization of the synthesized AgNPs was performed using a UV-Vis Spectrophotometer, Particle Size Analyzer (PSA), Transmission Electron Microscopy (TEM), and Fourier Transform Infra-Red (FTIR). The optimum conditions for AgNPs in this study were a sonication time of 70 minutes, a sonication temperature of 40°C, and a honey concentration of 10 ppm. The silver nanoparticles produced were bright yellow transparent dispersion, spherical in shape, with an average size of 25.7 nm, and a polydispersity index (PDI) of 0.2738. The AgNPs obtained from the optimization were tested for antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazil). The antioxidant activity of the optimized AgNPs had an IC50 value of 29.56 ppm, indicating that they are very strong inhibitors of DPPH free radicals.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Nanopartikel perak, Box-Behnken Design, Response Surface Methodology, Aktivitas antioksidan, Silver nanoparticles, Box-Behnken Design, Response Surface Methodology, Antioxidant activity
Subjects:	T Technology > TP Chemical technology > TP155.7 Chemical processes. T Technology > TP Chemical technology > TP159.A5 Antioxidants T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Shofiyah Edliana Tsaniputri
Date Deposited:	04 Aug 2025 11:33
Last Modified:	04 Aug 2025 11:33
URI:	http://repository.its.ac.id/id/eprint/127214

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