Optimasi Biodekolorisasi Metilen Biru oleh Ralstonia pickettii yang Terimobilisasi pada Matriks SA/PVA/Bentonit dengan Response Surface Methodology

Astiti, Audrey Ayudya (2025) Optimasi Biodekolorisasi Metilen Biru oleh Ralstonia pickettii yang Terimobilisasi pada Matriks SA/PVA/Bentonit dengan Response Surface Methodology. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pencemaran lingkungan akibat limbah pewarna tekstil, terutama metilen biru (MB), menjadi masalah serius karena karakteristiknya yang beracun dan sulit mengalami degradasi. Studi ini memiliki tujuan untuk mengoptimalkan proses diodekolorisasi MB dengan menggunakan Ralstonia pickettii yang diimobilisasi dalam matriks SA/PVA/bentonit melalui pendekatan Response Surface Methodology (RSM). Desain eksperimental yang diterapkan adalah Box Behnken Design (BBD) dengan tiga variabel bebas: pH, suhu, dan waktu inkubasi. Respon yang diharapkan adalah persentase dekolorisasi. Faktor pH dan suhu serta interaksi antara pH–suhu dan pH–waktu memiliki dampak yang signifikan terhadap proses dekolorisasi. Nilai LOF memiliki p-value < 0,0001 yang menunjukkan bahwa model memerlukan pengembangan lebih lanjut. Berdasarkan solusi optimasi, kondisi terbaik yang ditemukan adalah pH 5,5, suhu 35,50 °C, dan waktu inkubasi selama 11 hari dengan efisiensi dekolorisasi sebesar 98,88%. Asam borat sebagai crosslinker tambahan juga berpengaruh terhadap siklus reusabilitas beads. Beads dapat digunakan kembali hingga 4 siklus dengan waktu inkubasi selama 11 hari dalam 1 siklus. Temuan ini mengindikasikan bahwa sistem imobilisasi yang menggunakan SA/PVA/bentonit efektif dalam mendukung proses biodekolorisasi R. pickettii terhadap metilen biru serta penambahan asam borat pada crosslinker dapat memperkuat sifat mekanik dari beads.
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Environmental pollution caused by textile dye waste, particularly methylene blue (MB), poses a serious problem due to its toxic nature and resistance to degradation. This study aimed to optimize the biodecolorization process of MB using Ralstonia pickettii immobilized in an SA/PVA/bentonite matrix through the application of Response Surface Methodology (RSM). The experimental design employed was the Box-Behnken Design (BBD), involving three independent variables: pH, temperature, and incubation time. The desired response was the percentage of decolorization. Both pH and temperature, as well as the interactions between pH–temperature and pH–incubation time, were found to significantly affect the decolorization process. The lack-of-fit (LOF) test yielded a p-value of < 0.0001, indicating that further refinement of the model is required. Based on the optimization solution, the optimal conditions were identified as pH 5.5, temperature 35,50 °C, and an incubation period of 11 days, resulting in a decolorization efficiency of 98.88%. Boric acid, used as an additional crosslinker, also influenced the reusability cycle of the beads. The immobilized beads remained effective for up to four reuse cycles, with each cycle requiring 11 days of incubation. These findings indicatedthat the SA/PVA/bentonite immobilization system is effective in supporting the biodecolorization activity of R. pickettii against MB, and that the addition of boric acid enhances the mechanical strength of the beads.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Imobilisasi, Ralstonia pickettii, RSM, Metilen Biru, Biodecolorization, Immobilization, Ralstonia pickettii, RSM, Methylene Blue
Subjects:	Q Science Q Science > Q Science (General) Q Science > QD Chemistry > QD117 Absorption Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Audrey Ayudya Astiti
Date Deposited:	05 Aug 2025 00:51
Last Modified:	05 Aug 2025 00:51
URI:	http://repository.its.ac.id/id/eprint/127250

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