Yuniar, Ashila Salma (2025) Optimasi Biodekolorisasi Metilen Biru oleh Bakteri Bacillus subtilis yang Terimobilisasi pada Matriks SA-PVA-BENTONIT dengan Response Surface Methodology. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5004211108-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5004211108-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (23MB) | Request a copy |
Abstract
Pencemaran lingkungan akibat limbah pewarna sintetik seperti metilen biru (MB) menjadi permasalahan serius karena sifat toksiknya, sifat karsinogenik, dan kesulitan untuk terdegradasi. Salah satu metode penanganan yang ramah lingkungan adalah melalui proses biodekolorisasi menggunakan mikroorganisme. Penelitian ini bertujuan untuk mengkaji kemampuan bakteri Bacillus subtilis yang terimobilisasi dalam matriks Sodium Alginate-Polyvinyl Alcohol-Bentonit (SA-PVA-Bentonit) dalam proses biodekolorisasi MB, serta menentukan kondisi optimum menggunakan metode Response Surface Methodology (RSM) dengan desain Box Behnken. Imobilisasi dilakukan menggunakan kombinasi CaCl₂ dan asam borat sebagai agen crosslinking untuk meningkatkan kekuatan mekanik dan stabilitas beads. Hasil penelitian menunjukkan bahwa beads SA-PVA-Bentonit yang mengandung B. subtilis mampu mendekolorisasi larutan MB dengan konsentrasi 50 mg/L hingga 99,5%. Kondisi optimum biodekolorisasi diperoleh pada pH 9,96, suhu 33,81 °C, dan waktu inkubasi selama 63,12 jam, dengan persentase dekolorisasi sebesar 97%. Uji kemampuan penggunaan ulang menunjukkan bahwa beads masih efektif digunakan hingga enam siklus tanpa penurunan efisiensi yang signifikan. Karakterisasi beads menggunakan analisis Fourier Transform Infrared (FTIR) dan Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX) mengonfirmasi keberhasilan proses imobilisasi serta menunjukkan perubahan struktur fisik dan kimia pada matriks. Hasil penelitian ini menunjukkan bahwa teknologi imobilisasi B. subtilis dalam matriks SA-PVA-Bentonit berpotensi diterapkan sebagai metode alternatif yang efektif dan berkelanjutan untuk pengolahan limbah pewarna sintetik berbasis proses biodekolorisasi.
====================================================================================================================================
Environmental pollution caused by synthetic dye waste such as methylene blue (MB) has become a serious problem due to its toxic, carcinogenic, and persistent nature. One of the environmentally friendly treatment methods is biodecolorization using microorganisms. This study aimed to evaluate the ability of bacterium Bacillus subtilis immobilized in a Sodium Alginate-Polyvinyl Alcohol-Bentonite (SA-PVA-Bentonite) matrix for the biodecolorization of MB, as well as to determine the optimum conditions using Response Surface Methodology (RSM) with a Box-Behnken design. Immobilization was carried out using a combination of CaCl₂ and boric acid as crosslinking agents to enhance the mechanical strength and stability of the beads. The results showed that B. subtilis-immobilized SA-PVA-Bentonite beads were able to decolorize MB solution with a concentration of 50 mg/L up to 99.5%. The optimum biodecolorization conditions were achieved at pH 9.96, temperature 33.81 °C, and an incubation time of 63.12 hours, with a decolorization percentage of 97%. Reusability tests demonstrated that the beads remained effective for up to six biodecolorization cycles without significant efficiency loss. Characterization of the immobilized beads using Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX) confirmed the success of the immobilization process and showed changes in the physical and chemical structure of the matrix. Overall, this study demonstrates that the immobilization of B. subtilis within an SA-PVA-Bentonite matrix has the potential to be applied as an effective, eco-friendly, and sustainable alternative for the treatment of synthetic dye wastewater through biodecolorization processes.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biodekolorisasi, Bacillus subtilis, Imobilisasi, SA-PVA-Bentonit, Metilen Biru, Response Surface Methodology ============================================================== Biodecolorization, Bacillus subtilis, Immobilization, SA-PVA-Bentonite, Methylene Blue, Response Surface Methodology. |
| Subjects: | Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry |
| Depositing User: | Ashila Salma Yuniar |
| Date Deposited: | 05 Aug 2025 10:05 |
| Last Modified: | 05 Aug 2025 10:05 |
| URI: | http://repository.its.ac.id/id/eprint/127437 |
Actions (login required)
 |
View Item |