Evaluasi Stabilitas Obat Nano α-Mangostin Terlapisi dengan Gelatin Ikan Bandeng dan Tertaut Silang dengan Glutaraldehida

Indriani, Cita (2025) Evaluasi Stabilitas Obat Nano α-Mangostin Terlapisi dengan Gelatin Ikan Bandeng dan Tertaut Silang dengan Glutaraldehida. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Senyawa α-mangostin merupakan turunan senyawa xanton yang terdapat dalam kulit buah manggis (Garcinia mangostana L.). Senyawa α-mangostin memiliki aktivitas antikanker yang mampu memicu apoptosis dan menghambat proliferasi sel kanker. Penggunaan α-mangostin secara konvensional masih terbatas akibat kelarutan yang rendah, stabilitas fisikokimia yang buruk, serta bioavailabilitas yang rendah sehingga mudah beragregasi dalam lingkungan biologis. Senyawa α-mangostin difabrikasi dalam skala nano dengan modifikasi permukaan melalui pelapisan gelatin ikan bandeng dan taut silang glutaraldehida untuk meningkatkan stabilitas dan kelarutanya dalam media biologis. Penelitian ini bertujuan untuk sintesis obat nano α-mangostin dengan metode re-presipitasi, skrining konsentrasi gelatin dan glutaraldehida, evaluasi pengaruh adanya modifikasi permukaan dan pH terhadap stabilitas ukuran obat nano α-mangostin. Dispersibilitas obat nano α-mangostin diamati secara visual dan hamburan cahaya penyinaran laser pointer, ukuran partikelnya dikarakterisasi dengan Particle Size Analyzer (PSA), dan morfologinya dikarakterisasi dengan Field Emission Scanning Electron Microscope (FE-SEM). Hasil penelitian menunjukkan pelapisan gelatin ikan bandeng 250 µg/mL dan taut silang glutaraldehida 0,04% merupakan formulasi yang optimal mampu meningkatkan stabilitas ukuran partikel obat nano α-mangostin tetap pada rentang efek EPR dalam dispersi yaitu sebesar 168,7 nm dan PI 0,5984 dengan morfologi berbentuk persegi dengan ukuran yang seragam. Ukuran rata-rata partikel obat nano α-mangostin terlapisi gelatin ikan bandeng dan tertaut silang glutaraldehida dalam dispersi pada pH 7 sebesar 483,9 nm, sedangkan pada pH 5 sebesar 264,5 nm. Modifikasi permukaan nano obat α-mangostin belum mampu memaksimalkan peningkatan stabilitas dari perubahan pH.
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The α-mangostin compound is a derivative of xanthone compounds found in mangosteen fruit peel (Garcinia mangostana L.). The α-mangostin compound has anticancer activity that can trigger apoptosis and inhibit cancer cell proliferation. The conventional use of α-mangostin is still limited due to low solubility, poor physicochemical stability, and low bioavailability so that it easily aggregates in the biological environment. The compound α-mangostin was fabricated in nanoscale by surface modification through milkfish gelatin coating and glutaraldehyde crosslinking to improve its stability and solubility in biological media. This study aims to synthesize α-mangostin nanodrugs using the re-precipitation method, screening gelatin and glutaraldehyde concentrations, evaluating the effect of surface and pH modifications on the size stability of α-mangostin nanodrugs. Dispersibility of α-mangostin nanomedicine was observed by visual and laser pointer light scattering, its particle size was characterized by Particle Size Analyzer (PSA), and its morphology was characterized by Field Emission Scanning Electron Microscope (FE-SEM). The results showed that the coating of 250 µg/mL milkfish gelatin and 0.04% glutaraldehyde crosslinking was the optimal formulation capable of increasing the particle size stability of α-mangostin nanoparticles to remain in the EPR effect range in the dispersion of 168.7 nm and PI 0.5984 with a square-shaped morphology with uniform size. The average particle size of α-mangostin nanomedicine coated with milkfish gelatin and crosslinked with glutaraldehyde in dispersion at pH 7 was 483.9 nm, while at pH 5 it was 264.5 nm. The surface modification of α-mangostin nanomedicine has not been able to maximize the increase in stability from pH change.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	α-mangostin, Gelatin, Glutaraldehida, Modifikasi permukaan, Stabilitas, α-mangosteen, Glutaraldehyde, Surface modification, Stability
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Cita Indriani
Date Deposited:	05 Aug 2025 12:01
Last Modified:	05 Aug 2025 12:01
URI:	http://repository.its.ac.id/id/eprint/127498

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