Biodekolorisasi Metil Oranye Oleh Unbacterium Strain BS2049 Yang Terimobilisasi Oleh Matriks SA-PVA

Rivelna, Anastasia (2025) Biodekolorisasi Metil Oranye Oleh Unbacterium Strain BS2049 Yang Terimobilisasi Oleh Matriks SA-PVA. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil oranye (MO) merupakan salah satu pewarna azo sintetis yang banyak digunakan Dalam industri tekstil, namun keberadaannya mencemari perairan karena bersifat toksik, stabil, dan sulit terurai secara alami akibat adanya ikatan azo (–N=N–) dalam struktur molekulnya. Oleh karena itu, diperlukan metode pengolahan yang efektif sebelum limbah yang mengandung MO dibuang ke lingkungan. Penelitian ini bertujuan mengevaluasi kemampuan uncultured bacterium strain JN4 asal lahar Semeru dalam mendegradasi MO, baik dalam bentuk free cell maupun sistem terimobilisasi. Teknik imobilisasi dilakukan menggunakan matriks natrium alginat (SA) dan polivinil alkohol (PVA), dengan dua perlakuan yaitu beads SA–PVA kosong dan beads SA–PVA mengandung JN4. Hasil uji menunjukkan bahwa free cell JN4 mampu mendekolorisasi MO hingga 58%, namun tidak dapat digunakan ulang. Sementara itu, sistem imobilisasi SA–PVA–JN4 menunjukkan efisiensi dekolorisasi sebesar 83% yang disebabkan oleh degradasi struktur azo oleh bakteri, sedangkan SA–PVA kosong hanya mencapai 39%. Perbedaan ini mengindikasikan bahwa aktivitas biologis bakteri lebih efektif dalam mendegradasi struktur kimia MO dibandingkan mekanisme adsorpsi fisik semata. Kedua jenis beads menunjukkan stabilitas fisik dan dapat digunakan ulang hingga tiga siklus. Hasil FTIR menunjukkan bahwa spektrum utama tidak mengalami pergeseran pita yang signifikan, yang mengindikasikan struktur kimia matriks tetap stabil selama proses dekolorisasi. Dengan demikian, sistem imobilisasi SA–PVA–JN4 berpotensi sebagai metode bioremediasi yang efisien dan berkelanjutan untuk pengolahan limbah zat warna azo
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Methyl orange (MO) is a synthetic azo dye widely used in the textile industry, but its presence pollutes waters because it is toxic, stable, and difficult to decompose naturally due to the presence of azo bonds (–N=N–) in its molecular structure. Therefore, an effective treatment method is needed before waste containing MO is discharged into the environment. This study aims to evaluate the ability of uncultured bacterium strain JN4 from Semeru lava to degrade MO, both in the form of free cells and immobilized systems. The immobilization technique was carried out using sodium alginate (SA) and polyvinyl alcohol (PVA) matrices, with two treatments: empty SA–PVA beads and SA–PVA beads containing JN4. The test results showed that free cell JN4 was able to decolorize MO up to 58%, but could not be reused. Meanwhile, the SA–PVA–JN4 immobilization system showed a decolorization efficiency of 83% caused by the degradation of the azo structure by bacteria, while the empty SA–PVA only reached 39%. This difference indicates that the biological activity of bacteria is more effective in degrading the chemical structure of MO than the physical adsorption mechanism alone. Both types of beads exhibited physical stability and could be reused up to three cycles. FTIR results showed that the main spectrum did not experience a significant band shift, indicating that the chemical structure of the matrix remained stable during the decolorization process. Thus, the SA–PVA–JN4 immobilization system has the potential to be an efficient and sustainable bioremediation method for the treatment of azo dye wastewater

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Metil Oranye, Polivinil Alkohol, Sodium Alginat, Uncultured Bacterium Strain BS2049
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Anastasia Rivelna
Date Deposited:	06 Aug 2025 02:10
Last Modified:	06 Aug 2025 02:10
URI:	http://repository.its.ac.id/id/eprint/127617

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