Evaluasi Stabilitas Nanodispersi γ-Mangostin Termodifikasi Pektin Dengan Crosslinking Glutaraldehida

Putra, Ananda Satria Eka (2026) Evaluasi Stabilitas Nanodispersi γ-Mangostin Termodifikasi Pektin Dengan Crosslinking Glutaraldehida. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

γ-mangostin adalah senyawa xanton bioaktif menunjukkan aktivitas farmakologis yang cocok sebagai obat antikanker melalui pemanfaatan fenomena efek EPR. Namun, formulasi pada rentang ukuran tersebut terbatas karena stabilitas fisikokimia yang rendah. Oleh karena itu, penelitian ini bertujuan untuk menemukan formulasi γ-mangostin yang stabil terhadap berbagai kondisi fisiologis dan penyimpanan melalui metode re-presipitasi dengan memanfaatkan pektin sebagai bahan coating dan glutaraldehid sebagai agen crosslinking. Nanodispersi γ-mangostin disiapkan melalui metode re-presipitasi larutan γ-mangostin ke dalam larutan pektin, diikuti dengan crosslinking glutaraldehida. Karakterisasi fisikokimia menggunakan FESEM untuk analisis morfologi, DLS untuk pengukuran ukuran partikel, dan FTIR untuk analisis gugus fungsional. Pengujian tambahan juga dilakukan seperti efisiensi pelapisan pektin, stabilitas terhadap perubahan pH, redispersibilitas, dan stabilitas penyimpanan. Hasil menunjukkan bahwa γ-mangostin tanpa coating beragregasi (657,7 nm; PDI ≈ 1) dan zeta potensial sebesar -13,59 mV. Pelapisan coating pektin pada konsentrasi optimal 10 µg/mL menghasilkan nanopartikel stabil (155,9 nm dan PDI 0,3172), meskipun efisiensi coating relatif rendah (0,73%). Proses Crosslinking glutaraldehida (0,1%) menunjukkan morfologi seperti jaringan yang saling terhubung yang menunjukkan matriks pektin terikat silang tiga dimensi dengan ukuran partikel yang stabil (136,1 nm; PDI 0,3978), dan zeta potensial (-21,85 mV). Analisis FTIR mengkonfirmasi keberhasilan coating dan crosslinking melalui pelebaran pita -OH (3600-3200 cm-1), C=O xanton (1650 cm-1), pita ester C=O (1630-1600 cm-1), dan C-O-C yang lebih tajam (1000 cm-1). Studi stabilitas menunjukkan bahwa formulasi GM-Pektin-GA tetap stabil dalam pH 5 (266,6 nm; 0,350) dan pH 7 (183 nm; PDI 0,415). Nanodispersi juga memiliki redispersibilitas yang baik (183,1 nm; PDI 0,268), dan mempertahankan ukuran partikel tetap stabil (184 nm) setelah 30 hari penyimpanan pada suhu 4 °C. Secara keseluruhan, nanodispersi γ-mangostin berbasis pektin yang diperkuat dengan crosslinking glutaraldehida menunjukkan potensi yang kuat untuk pengembangan lebih lanjut sebagai sistem penghantaran obat berbasis nanoteknologi.
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γ-mangostin is a bioactive xanthone compound that exhibits suitable pharmacological activity as an anticancer drug through the utilization of the EPR effect phenomenon. However, formulations in this size range are limited due to low physicochemical stability. Therefore, this study aims to find a stable γ-mangostin formulation under various physiological and storage conditions through a re-precipitation method using pectin as a coating material and glutaraldehyde as a crosslinking agent. γ-mangostin nanodispersions were prepared by re-precipitation of γ-mangostin solution into pectin solution, followed by glutaraldehyde crosslinking. Physicochemical characterization used FESEM for morphological analysis, DLS for particle size measurement, and FTIR for functional group analysis. Additional tests were also carried out such as pectin coating efficiency, stability to pH changes, redispersibility, and storage stability. The results showed that uncoated γ-mangostin aggregated (657.7 nm; PDI ≈ 1) and had a zeta potential of -13.59 mV. Pectin coating at an optimal concentration of 10 µg/mL produced stable nanoparticles (155.9 nm and PDI 0.3172), although the coating efficiency was relatively low (0.73%). Glutaraldehyde (0.1%) crosslinking process showed a network-like morphology that indicated a three-dimensional crosslinked pectin matrix with a stable particle size (136.1 nm; PDI 0.3978), and a zeta potential (-21.85 mV). FTIR analysis confirmed the successful coating and crosslinking through broadening of the -OH band (3600-3200 cm-1), C=O xanthone (1650 cm-1), C=O ester band (1630-1600 cm-1), and sharper C-O-C (1000 cm-1). Stability studies showed that the GM-Pectin-GA formulation remained stable at pH 5 (266.6 nm; 0.350) and pH 7 (183 nm; PDI 0.415). The nanodispersion also had good redispersibility (183.1 nm; PDI 0.268), and maintained a stable particle size (184 nm) after 30 days of storage at 4 °C. Overall, the pectin-based γ-mangostin nanodispersion reinforced with glutaraldehyde crosslinking showed strong potential for further development as a nanotechnology-based drug delivery system.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	γ-mangostin, glutaraldehida, nanodispersi, nanodrug delivery, pektin, γ-mangostin, glutaraldehyde, nanodispersion, nanodrug delivery, pectin
Subjects:	T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles. T Technology > TP Chemical technology > TP248.25.N35 Microencapsulation.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Ananda Satria Eka Putra
Date Deposited:	02 Feb 2026 08:44
Last Modified:	02 Feb 2026 08:44
URI:	http://repository.its.ac.id/id/eprint/131562

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