Identifikasi Metabolit Dari Biodegradasi Metilen Biru Oleh Kultur Campuran Jamur Dan Bakteri Yang Terimobilisasi

Yuniarti, Eka Pratiwi (2022) Identifikasi Metabolit Dari Biodegradasi Metilen Biru Oleh Kultur Campuran Jamur Dan Bakteri Yang Terimobilisasi. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pewarna tekstil Metilen Biru (MB) adalah salah satu penyumbang limbah cair terbesar yang belum dilakukan dengan baik. Biodegradasi merupakan salah satu upaya penanganan limbah dengan memanfaatkan peran mikroorganisme. Pada penelitian ini, telah dilakukan biodegradasi MB oleh kultur campuran jamur T. viride dan bakteri P. aeruginosa yang terimobilisasi dalam matriks natrium alginat-polivinil alkohol-bentonit (SA-PVA-bentonit) dalam bentuk beads. Biodegradasi MB oleh kultur campuran yang terimobilisasi mencapai persentase dekolorisasi MB tertinggi yaitu 96,25%, lebih tinggi daripada kultur campuran sel bebas yaitu 94,25%. Berdasarkan data optimasi biodegradasi MB, diperoleh kondisi optimal berada pada suhu 55 °C, waktu inkubasi selama 39 jam, dengan konsentrasi larutan MB mula-mula 50 mg/L mencapai persentase dekolorisasi MB sebesar 99,48%. Kemampuan penggunaan kembali beads yang telah digunakan diuji dalam kondisi optimal menghasilkan 3 kali siklus dengan persentase degradasi yang terus menurun dari 99,38%, 92,07%, dan 80,01% hingga beads tidak dapat digunakan kembali. Dari proses biodegradasi MB ini dihasilkan empat metabolit produk yaitu azure A, azure C, C16H20N3S, dan C16H21N3SO. Imobilisasi kultur campuran efektif dalam proses biodegradasi MB dan penggunaannya dapat dilakukan secara berulang.
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Methylene Blue (MB) textile dye is one of the biggest contributors to wastewater which has not been done well. Biodegradation is one of the efforts to handle waste by utilizing the role of microorganisms. In this study, MB biodegradation was carried out by mixed culture of T. viride and P. aeruginosa bacteria immobilized in a sodium alginate-polyvinyl alcohol-bentonite (SA-PVA-bentonite) matrix in the form of beads. The biodegradation of MB by immobilized mixed culture reached the highest percentage of MB decolorization, which was 96,25%, higher than that of mixed culture of free cells, which was 94,25%. Based on MB biodegradation optimization data, the optimal condition was obtained at 55 °C, incubation time of 39 hours, with the initial MB solution concentration of 50 mg/L reaching the MB decolorization percentage of 99,48%. The ability to reuse beads that have been used was tested under optimal conditions resulting in 3 cycles with the percentage of degradation that continued to decrease from 99,38%, 92,07%, and 80,01% until the beads could not be reused. From this MB biodegradation process, four product metabolites are produced, namely azure A, azure C, C16H20N3S, and C16H21N3SO. Mixed culture immobilization is effective in MB biodegradation process and its use can be repeated.

Item Type:	Thesis (Masters)
Additional Information:	RTKi 547.135 Yun i-1 2022
Uncontrolled Keywords:	Biodegradasi, Metilen biru, Imobilisasi, Trichoderma viride, Pseudomonas aeruginosa, Biodegradation, Methylene blue, Immobilization, Trichoderma viride, Pseudomonas aeruginosa.
Subjects:	Q Science > QP Physiology > QP517.B5 Biodegradation.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	04 May 2026 02:48
Last Modified:	04 May 2026 02:48
URI:	http://repository.its.ac.id/id/eprint/132951

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