Biodekolorisasi Metil Oranye Oleh Bakteri Bacillus subtilis Yang Terimobilisasi DALAM Matriks SA-PVA-BENTONIT.

Hairunissa, Frida Wahyu (2022) Biodekolorisasi Metil Oranye Oleh Bakteri Bacillus subtilis Yang Terimobilisasi DALAM Matriks SA-PVA-BENTONIT. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil Oranye (MO) merupakan salah satu pewarna yang umum digunakan dalam industri tekstil. Limbah MO yang dibuang langsung ke lingkungan akan menimbulkan bahaya bagi lingkungan dan manusia dikarenakan bersifat toksik dan karsinogenik, sehingga diperlukan penanganan yang tepat. Penelitian ini mempelajari biodekolorisasi pewarna MO oleh bakteri Bacillus subtilis (BS) yang terimobilisasi dalam matriks Sodium Alginate-polivinil alkohol-bentonit (SA-PVA-Bentonit). Sistem imobilisasi menggunakan metode entrapment, dengan komposisi matriks SA 2%-PVA 4%-Bentonit 1% (b/v). Proses dekolorisasi MO dilakukan dengan metode free cell dan imobilisasi, dengan variasi perlakuan bakteri hidup dan mati. Biodekolorisasi MO dilakukan pada media air dengan konsentrasi akhir 50 mg/L dalam keadaan statis dan diamati selama 10 hari. Hasil penelitian menunjukkan keunggulan beads SA-PVA-Bentonit-BS hidup dalam penghilangan MO dengan persen dekolorisasi yang paling tinggi yaitu 95,65%, sedangkan untuk free cell bakteri hidup hanya mampu mendekolorisasi sebesar 34,61%. Beads imobilisasi memiliki reusability yang baik setelah empat siklus dekolorisasi MO dengan persen dekolorisasi sebesar 95,65; 65,55; 40,87; dan 26,43%. Karakterisasi FTIR dilakukan untuk mengidentifikasi gugus fungsi, sedangkan analisa SEM-EDX untuk melihat kondisi topografi serta unsur-unsur yang terdapat dalam beads. Analisa FTIR menunjukkan adanya pemutusan ikatan azo serta keberadaan SA, PVA, dan Bentonit dalam matriks. Kemudian hasil karakterisasi SEM menunjukkan adanya perubahan struktur beads antara sebelum dan setelah proses dekolorisasi. Hasil ini menunjukkan bahwa B. subtilis yang terimobilisasi dalam matriks SA-PVA-Bentonit mampu mendekolorisasi MO secara efektif dan dapat digunakan secara berulang.
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Methyl Orange (MO) is one of the commonly used dyes in the textile industry. MO waste that is disposed of directly into the environment pose a danger to the environment and humans because it is toxic and carcinogenic, so proper handling is needed. This research studied the biodecolorization of MO dye by Bacillus subtilis (BS) bacterium immobilized in Sodium Alginate-polyvinyl alcohol-bentonite (SA-PVA-Bentonite) matrix. The immobilization system used the entrapment method, with a matrix composition of SA 2%-PVA 4%-Bentonite 1% (w/v). The MO decolorization process was carried out using the free cell and immobilization methods, with variations in the treatment of live and dead bacteria. MO biodecolorization was carried out in aqueous media with a final concentration of 50 mg/L under static conditions and observed for 10 days. The results showed that the immobilized of living bacteria in SA-PVA-Bentonite has highest percentage of decolorization, which was 95.65%, while for free cell living bacteria were only able to decolorize as much as 34.61%. The immobilized beads had good reusability after four cycles of MO decolorization, with percent decolorization of 95.65; 65.55; 40.87; and 26.43%. The FTIR characterization was carried out to identify the functional groups, while the SEM-EDX analysis was to see the topographical conditions and the elements contained in the beads. FTIR analysis showed the presence of azo cleavage and the presence of SA, PVA, and Bentonite in the matrix. Then the results of SEM characterization showed a change in the structure of the beads between before and after the decolorization process. These results indicate that B. subtilis immobilized in SA-PVA-Bentonite matrix is able to decolorize MO effectively and can be used repeatedly.

Item Type:	Thesis (Other)
Additional Information:	RSKi 547.135 Hai b-1 2022
Uncontrolled Keywords:	Bacillus subtilis, Biodegradasi, Biodekolorisasi, Metil Oranye, Imobilisasi. Bacillus subtilis, Biodegradation, Biodecolorization, Methyl Orange, Immobilization.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	05 May 2026 07:17
Last Modified:	05 May 2026 07:17
URI:	http://repository.its.ac.id/id/eprint/132991

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