Decolorization Of Methyl Orange By Immobilized Bacterium Pseudomonas aeruginosa Into SA/PVA/ACTIVATED Carbon.

Shofaa, Salsabiila Kaamiliaa (2022) Decolorization Of Methyl Orange By Immobilized Bacterium Pseudomonas aeruginosa Into SA/PVA/ACTIVATED Carbon. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil oranye (MO) merupakan salah satu pewarna yang banyak digunakan dalam industri tekstil. Limbah pewarna MO memiliki efek berbahaya bagi lingkungan dan manusia karena sifatnya sulit didegradasi. Bakteri P. aeruginosa dapat digunakan secara efektif dalam pengolahan limbah pewarna MO. Namun, terdapat kekurangan dalam penggunaannya sebagai agen pendegradasi seperti kekuatan mekanik yang rendah, kepadatan sel yang rendah, dan kesulitan dalam pemisahan limbah biomassa. Oleh karena itu, metode imobilisasi mikroorganisme dapat digunakan dalam pengolahan limbah pewarna MO dikarenakan dalam metode ini relatif mudah dalam pemisahan sel dari bahan pembawa dan dapat digunakan kembali. Penelitian ini membahas tentang biodekolorisasi MO oleh bakteri Pseudomonas aeruginosa yang terimobilisasi pada matriks SA/PVA/Arang Aktif. Pada penelitian ini bakteri P. aeruginosa diimobilisasi dalam matriks SA/PVA/Arang Aktif dengan komposisi secara berturut turut 2%/4%/1% (b/v). Proses biodekolorisasi MO dilakukan dengan dua metode yakni free cell dan imobilisasi dengan waktu inkubasi selama 10 hari dalam kondisi statis. Hasil dekolorisasi yang menunjukkan bahwa metode imobilisasi lebih maksimal yakni sebesar 66,88% sedangkan free cell sebesar 43,28%. Keberhasilan biodekolorisasi MO dengan pada beads imobilisasi dapat dikonfirmasi dengan spektra FTIR dan visualisasi SEM. Uji penggunaan kembali menujukkan bahwa beads imobilisasi dapat digunakan kembali hingga dua kali siklus. Hasil ini menunjukkan bahwa P. aeruginosa yang terimobilisasi pada matriks SA/PVA/Arang Aktif dapat digunakan sebagai alternatif baru untuk mendekolorisasi MO dan mengurangi permasalahan limbah yang mencemari lingkungan.
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Methyl orange (MO) is a dye that is widely used in the textile industry. MO dye waste has harmful effects on the environment and humans because it is difficult to degrade. Bacterium P. aeruginosa can be used effectively in the treatment of MO dye waste. However, there are weakness to used it as a degrading agent such as low mechanical strength, low cell density, and difficulty in separating waste biomass. Therefore, the method of immobilization of microorganisms can be used in the treatment of MO dye waste because in this method it is relatively easy to separate cells from the carrier material and can be reused. This study discusses the biodecolorization of MO by immobilized Pseudomonas aeruginosa bacteria into SA/PVA/Activated carbon matrix. The entrapment method is a bacterial immobilization method used in this study in a matrix of SA/PVA/Activated carbon with a composition of 2%/4%/1% (w/v). The MO biodecolorization process was carried out by two methods, namely free cell and immobilization with an incubation time of 10 days under static conditions. The results of decolorization showed that the immobilization method was more optimal with a percentage of 66.88% while the free cells were 43.28%. The success of MO biodecolorization with immobilized beads can be confirmed by FTIR spectra and SEM visualization. The reuse test showed that the immobilized beads could be reused for two cycles. These results indicate that P. aeruginosa immobilized on the SA/PVA/Activated carbon matrix can be used as a new alternative to docolorization of MO and reduce the problem of waste that pollutes the environment.

Item Type:	Thesis (Other)
Additional Information:	RSKi 547.135 Sho b-1 2022
Uncontrolled Keywords:	Pseudomonas aeruginosa, Imobilisasi, Biodekolorisasi, Metil Oranye, Arang aktif. Pseudomonas aeruginosa, Immobilization, Biodecolorization, Methyl Orange, Acrtivated Carbon.
Subjects:	Q Science > QP Physiology > QP517.B5 Biodegradation.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	07 May 2026 01:59
Last Modified:	08 May 2026 02:10
URI:	http://repository.its.ac.id/id/eprint/133031

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