Deteksi Tetrasiklin Menggunakan Screen-Printed Carbon Electrode Termodifikasi Nanopartikel Magnetik Dan Deep Eutectic Solvent

Cholidah, Tsana (2026) Deteksi Tetrasiklin Menggunakan Screen-Printed Carbon Electrode Termodifikasi Nanopartikel Magnetik Dan Deep Eutectic Solvent. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penelitian ini menggunakan screen-printed carbon electrode (SPCE) termodifikasi nanopartikel magnetik (MNP) dan deep eutectic solvent (DES) untuk meningkatkan sensitivitas deteksi tetrasiklin. Modifikasi elektroda dilakukan dengan metode drop-casting dan selanjutnya elektroda dikarakterisasi dengan FESEM-EDX. Kinerja elektroda dievaluasi dengan metode voltametri siklik (CV). Hasil karakterisasi FESEM menunjukkan perubahan morfologi permukaan setelah proses modifikasi. SPCE tanpa modifikasi memiliki permukaan rata dan tidak bertekstur dengan karbon sebagai unsur dominan. SPCE/DES menunjukkan lapisan tipis DES yang menutupi elektroda, sedangkan analisis EDX mengidentifikasi karbon sebagai unsur dominan yang mengindikasikan bahwa DES tersusun atas senyawa berbasis karbon. SPCE/MNP menunjukkan distribusi partikel MNP pada permukaan elektroda yang dikonfirmasi oleh keberadaan unsur Fe dan O berdasarkan analisis EDX. SPCE/MNP-DES menunjukkan distribusi nanopartikel magnetik yang merata dan terlapisi DES. Analisis EDX mengonfirmasi keberadaan unsur Fe dan O serta peningkatan persentase berat karbon, yang menunjukkan bahwa MNP berhasil terdispersi dalam DES. Hasil penelitian CV menunjukkan bahwa SPCE/MNP-DES memberikan kinerja terbaik dengan rentang linier 0,1-1 mM, sensitivitas sebesar 6,76494 mA·mM-1·cm², limit deteksi (LOD) sebesar 0,1114 mM, dan limit kuantifikasi (LOQ) sebesar 0,3714 mM pada pH 4 dengan laju pindai 100 mV/s. Hubungan linier antara arus puncak redoks dan akar kuadrat laju pindai (R2 = 0,999) menunjukkan bahwa proses dikendalikan oleh difusi. Analisis log arus-log potensial menghasilkan nilai kemiringan 0,8669 (anodik) dan 0,8611 (katodik), yang menunjukkan sistem berlangsung melalui mekanisme kontrol campuran difusi dan adsorpsi. Analisis parameter Nicholson menghasilkan nilai konstanta laju transfer elektron standar (k⁰) pada rentang 10⁻⁴ hingga 10⁻³ cm s⁻¹ seiring peningkatan laju pindai dari 25 hingga 100 mV s⁻¹, yang menunjukkan karakteristik sistem kuasi-reversibel. Elektroda termodifikasi juga menunjukkan selektivitas yang baik terhadap tetrasiklin serta stabilitas yang baik dengan nilai RSD kurang dari 5% selama tujuh hari pengujian. Hasil ini menunjukkan bahwa kombinasi MNP dan DES mampu meningkatkan performa SPCE sehingga berpotensi digunakan sebagai platform deteksi tetrasiklin yang cepat, sensitif, dan andal.
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This study employed a screen-printed carbon electrode (SPCE) modified with magnetic nanoparticles (MNP) and a deep eutectic solvent (DES) to improve the sensitivity of tetracycline detection. The modification process applied the drop-casting method, and the characterization process utilized FESEM-EDX. Cyclic voltammetry (CV) evaluated the electrochemical performance of the modified electrode. The FESEM characterization revealed changes in the surface morphology after the modification process. The unmodified SPCE exhibited a smooth and non-textured surface with carbon as the dominant element. The SPCE/DES exhibited a thin DES layer covering the electrode surface. The EDX analysis identified carbon as the dominant element, indicating that the DES mainly consisted of carbon-based compounds. The SPCE/MNP exhibited MNP particles distributed across the electrode surface. The EDX analysis confirmed the presence of Fe and O elements in the modified electrode. The SPCE/MNP-DES exhibited a homogeneous distribution of magnetic nanoparticles coated with DES. The EDX analysis confirmed the presence of Fe and O elements and revealed an increase in the carbon weight percentage, indicating the successful dispersion of MNPs within the DES matrix. The CV measurements demonstrated that the SPCE/MNP-DES exhibited the best electrochemical performance at pH 4 and a scan rate of 100 mV s⁻¹, with a linear range of 0.1–1 mM, a sensitivity of 6.76494 mA·mM⁻¹·cm⁻², a limit of detection (LOD) of 0.1114 mM, and a limit of quantification (LOQ) of 0.3714 mM. The linear relationship between the redox peak current and the square root of the scan rate (R² = 0.999) indicated a diffusion-controlled electrochemical process. The log current–log potential analysis produced slope values of 0.8669 for the anodic process and 0.8611 for the cathodic process. These values indicated that the system followed a mixed diffusion- and adsorption-controlled mechanism. The Nicholson analysis generated standard electron transfer rate constant (k⁰) values in the range of 10⁻⁴ to 10⁻³ cm s⁻¹ as the scan rate increased from 25 to 100 mV s⁻¹. These values indicated the quasi-reversible behavior of the electrochemical system. The modified electrode exhibited good selectivity toward tetracycline and maintained stable performance during the testing period. The stability test produced a relative standard deviation (RSD) value of less than 5% over seven days of measurement. These findings demonstrate that the combination of MNP and DES effectively enhances the performance of SPCE and provides a promising platform for rapid, sensitive, and reliable tetracycline detection.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Deep Eutectic Solvent, Nanopartikel Magnetik, Screen-Printed Carbon Electrode, Tetrasiklin, Voltametri Siklik; Cyclic Voltammetry, Deep Eutectic Solvent, Magnetic Nanoparticles, Screen-Printed Carbon Electrode, Tetracycline.
Subjects:	Q Science > QD Chemistry > QD115 Electrochemical analysis Q Science > QD Chemistry > QD553 Electrochemistry. Electrolysis Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Tsana Cholidah
Date Deposited:	28 Jul 2026 07:16
Last Modified:	28 Jul 2026 07:16
URI:	http://repository.its.ac.id/id/eprint/135447

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