Pengaruh Variasi Suhu dan Salinitas Terhadap Pembentukan Biofilm Konsorsium Bakteri Isolat BIO-B dan BIO-G pada Plastik LDPE

Chanan, Hafidza (2026) Pengaruh Variasi Suhu dan Salinitas Terhadap Pembentukan Biofilm Konsorsium Bakteri Isolat BIO-B dan BIO-G pada Plastik LDPE. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Peningkatan jumlah sampah plastik di Indonesia, yang mencapai 17,86% dari total timbunan sampah nasional, menunjukkan ancaman serius bagi lingkungan, terutama karena polimer seperti LDPE memiliki struktur kimia yang stabil dan sulit terdegradasi secara alami. Isolat BIO-B dan BIO-G yang diisolasi dari mangrove Wonorejo, Surabaya, dilaporkan mampu mendegradasi plastik LDPE dengan efektivitas berbeda, yaitu isolat BIO-B menurunkan berat plastik sekitar 2%, sedangkan BIO-G sekitar 8,9% selama masa inkubasi 4 minggu. Penelitian ini bertujuan untuk meningkatkan biodegradasi plastik dengan menggabungkan kedua isolat tersebut dan juga untuk mengetahui pengaruh suhu dan salinitas terhadap proses degradasinya. Variasi suhu yang digunakan adalah 30°C, 37°C, dan 45°C dengan salinitas 3‰, 15‰, dan 30‰. Pengujian dilakukan selama 12 minggu menggunakan media MSM, dengan parameter berupa pembentukan biofilm di permukaan plastik, pertumbuhan konsorsium di kolom air, dan analisis hidrofobisitas plastik. Data dianalisis menggunakan ANOVA dua arah, dilanjutkan dengan uji Tukey apabila terdapat perbedaan signifikan. Hasil menunjukkan bahwa pembentukan biofilm tertinggi diperoleh pada suhu 30°C dan salinitas 15‰, sedangkan pertumbuhan konsorsium di kolom air berlangsung lambat pada seluruh perlakuan dan paling lambat pada suhu 45°C. Analisis hidrofobisitas menunjukkan penurunan sifat hidrofobik LDPE pada hampir seluruh perlakuan dibandingkan kontrol negatif, dengan perubahan terbesar pada suhu 30°C dan salinitas 15‰, yaitu penurunan diameter tetesan minyak sebesar 14,6% dan peningkatan diameter tetesan air sebesar 18,2%.
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The increasing amount of plastic waste in Indonesia, which accounts for 17.86% of total national waste generation, indicates a serious threat to the environment, particularly because polymers such as LDPE have a stable chemical structure that is resistant to natural degradation. The BIO-B and BIO-G isolates, obtained from mangrove sediment in Wonorejo, Surabaya, have been reported to degrade LDPE plastic with different levels of effectiveness, with BIO-B reducing plastic weight by approximately 2%, while BIO-G achieved a reduction of approximately 8.9% over a 4-week incubation period. This study aimed to enhance plastic biodegradation by combining the two isolates and to determine the effect of temperature and salinity on the degradation process. The temperature variations used were 30°C, 37°C, and 45°C, combined with salinity levels of 3‰, 15‰, and 30‰. The experiment was conducted over 12 weeks using MSM media, with parameters including biofilm formation on the plastic surface, consortium growth in the water column, and hydrophobicity analysis of the plastic. Data were analyzed using two-way ANOVA, followed by Tukey's post hoc test in cases of significant differences. The results showed that the highest biofilm formation was obtained at 30°C and 15‰ salinity, whereas consortium growth in the water column was slow across all treatments and was slowest at 45°C. Hydrophobicity analysis revealed a decrease in the hydrophobic properties of LDPE in nearly all treatments compared to the negative control, with the greatest change observed at 30°C and 15‰ salinity, namely a 14.6% decrease in oil droplet diameter and an 18.2% increase in water droplet diameter.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodegradasi LDPE, Biofilm, BIO-B, BIO-G, Konsorsium Bakteri. Biodegradation LDPE, Biofilm, BIO-B, BIO-G, Bacterial Consortium.
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Hafidza Chanan
Date Deposited:	24 Jul 2026 02:04
Last Modified:	24 Jul 2026 02:04
URI:	http://repository.its.ac.id/id/eprint/136916

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