Pengembangan Dan Optimasi Scaffold Hidrogel Hidroksiapatit/Natrium Alginat/Polietilena Glikol Untuk Aplikasi Perbaikan Tulang Rawan Menggunakan Metodologi Permukaan Respon (Central Composite Design)

Wati, Eka Dewi Ratna and Mahendra, Achmad (2026) Pengembangan Dan Optimasi Scaffold Hidrogel Hidroksiapatit/Natrium Alginat/Polietilena Glikol Untuk Aplikasi Perbaikan Tulang Rawan Menggunakan Metodologi Permukaan Respon (Central Composite Design). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kerusakan tulang rawan merupakan salah satu permasalahan muskuloskeletal yang memiliki kemampuan regenerasi yang terbatas sehingga memerlukan biomaterial yang mampu mendukung proses perbaikan jaringan. Scaffold hidrogel berbasis hidroksiapatit (HAp), natrium alginat (SA), dan polietilena glikol (PEG) berpotensi menjadi kandidat material rekayasa jaringan karena memiliki sifat biokompatibel, hidrofilik, serta mampu menyerupai lingkungan ekstraseluler. Penelitian ini bertujuan mengembangkan dan mengoptimasi komposisi scaffold hidrogel HAp/SA/PEG untuk aplikasi perbaikan tulang rawan menggunakan Response Surface Methodology (RSM) dengan rancangan Central Composite Design (CCD). PEG berperan sebagai variabel tetap, SA dan HAp berperan sebagai variabel bebas, sedangkan respons yang diamati berupa nilai compressive strength, compressive modulus, dan volumetric swelling ratio. Scaffold disintesis melalui metode mixing diikuti proses crosslinking. Data hasil pengujian dianalisis menggunakan analisis varians (ANOVA) untuk mengevaluasi signifikansi model dan menentukan pengaruh masing-masing faktor terhadap karakteristik scaffold. Selanjutnya, optimasi dilakukan menggunakan fungsi desirability untuk memperoleh komposisi optimum yang memberikan keseimbangan antara kekuatan mekanik, kemampuan menyerap cairan, dan laju degradasi yang sesuai dengan kebutuhan regenerasi tulang rawan. Hasil penelitian diharapkan menghasilkan scaffold hidrogel dengan sifat fisik dan mekanik yang lebih optimal serta memberikan informasi mengenai hubungan antara komposisi material dan karakteristik scaffold. Penelitian ini diharapkan dapat menjadi dasar pengembangan biomaterial berbasis hidrogel sebagai kandidat scaffold yang menjanjikan untuk aplikasi rekayasa jaringan tulang rawan.
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Cartilage damage is one of the most common musculoskeletal disorders with a limited capacity for self-regeneration, thereby requiring biomaterials capable of supporting tissue repair. Hydrogel scaffolds based on hydroxyapatite (HAp), sodium alginate (SA), and polyethylene glycol (PEG) have attracted considerable attention as promising materials for tissue engineering due to their biocompatibility, hydrophilicity, and ability to mimic the extracellular matrix. This study aims to develop and optimize the composition of HAp/SA/PEG hydrogel scaffolds for cartilage repair applications using Response Surface Methodology (RSM) with a Central Composite Design (CCD). In this study, PEG was maintained as a fixed variable, while SA and HAp were treated as independent variables. The responses evaluated included compressive strength, compressive modulus, and volumetric swelling ratio. The hydrogel scaffolds were synthesized using a mixing method followed by a crosslinking process. The experimental data were analyzed using analysis of variance (ANOVA) to evaluate the significance of the developed model and determine the effects of each factor on the scaffold characteristics. Furthermore, optimization was performed using the desirability function to identify the optimum scaffold composition that provides a balanced combination of mechanical strength, fluid absorption capacity, and degradation behavior suitable for cartilage regeneration. The findings of this study are expected to produce hydrogel scaffolds with improved physical and mechanical properties while providing insights into the relationship between material composition and scaffold characteristics. This research is also expected to serve as a scientific basis for the development of hydrogel-based biomaterials as promising scaffold candidates for cartilage tissue engineering applications.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	CCD, Hidrogel, Hidroksiapatit, PEG, RSM. Cartilage, Hydrogel, Hydroxyapatite, Osteoarthritis, RSM
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP1140 Polymers T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering
Divisions:	Faculty of Vocational > 24305-Industrial Chemical Engineering Technology
Depositing User:	Achmad Mahendra
Date Deposited:	27 Jul 2026 07:03
Last Modified:	27 Jul 2026 07:03
URI:	http://repository.its.ac.id/id/eprint/137845

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