Kharisma, Yolanda Ely Dian (2026) Potensi Nanoemulsi Gaharu Merauke (Aquilaria Malaccensis) Sebagai Antibakteri Dan Antibiofilm Pada Bakteri Acinetobacter Baumannii Secara In Vitro Dan In Vivo. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Acinetobacter baumannii merupakan bakteri patogen oportunistik yang mampu membentuk biofilm dan resisten terhadap berbagai antibiotik sehingga menjadi salah satu penyebab infeksi nosokomial. Minyak gaharu Merauke (Aquilaria malaccensis) diketahui mengandung senyawa bioaktif yang berpotensi sebagai agen antimikroba. Pengujian dilakukan menggunakan metode Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), uji biofilm, uji toksisitas, dan uji proteksi pada larva G. mellonella menggunakan isolat A. baumannii 144 dan A. baumannii PMF. Hasil penelitian menunjukkan bahwa nanoemulsi minyak gaharu Merauke memiliki aktivitas antibakteri yang lemah (MIC > 500000 ppm) dan memiliki potensi antibiofilm dengan penghambatan kurang lebih sekitar 60% pada kedua isolat A. baumannii. Uji toksisitas menunjukkan bahwa nanoemulsi memiliki tingkat toksisitas yang rendah terhadap larva G. mellonella dengan nilai survival lebih dari 75% hingga hari ke-5. Pada uji proteksi, menunjukkan bahwa nanoemulsi tidak memiliki efek protektif terhadap infeksi A.baumannii PMF pada larva G. mellonella.
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Acinetobacter baumannii is an opportunistic pathogenic bacterium capable of forming biofilms and exhibiting resistance to multiple antibiotics, making it one of the major causes of nosocomial infections. Merauke agarwood (Aquilaria malaccensis) oil is known to contain bioactive compounds with potential antimicrobial properties. This study evaluated the antibacterial and antibiofilm activities of Merauke agarwood oil nanoemulsion using the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), biofilm assay, toxicity assay, and protection assay in Galleria mellonella larvae against A. baumannii 144 and A. baumannii PMF isolates. The results showed that the Merauke agarwood oil nanoemulsion exhibited weak antibacterial activity (MIC > 500000 ppm) but demonstrated antibiofilm potential, inhibiting biofilm formation by approximately 60% in both A. baumannii isolates. The toxicity assay indicated that the nanoemulsion exhibited low toxicity toward G. mellonella larvae, with a survival rate of more than 75% up to day 5. However, the protection assay demonstrated that the nanoemulsion did not provide a protective effect against A. baumannii PMF infection in G. mellonella larvae.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Acinetobacter baumannii, Antibiofilm, Aquilaria malaccensis, Galleria mellonella, Nanoemulsion.=========================================================================================================== Acinetobacter baumannii, Antibiofilm, Aquilaria malaccensis, Galleria mellonella, Nanoemulsi. |
| Subjects: | Q Science Q Science > QH Biology Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yolanda Ely Dian Kharisma |
| Date Deposited: | 27 Jul 2026 06:46 |
| Last Modified: | 27 Jul 2026 06:46 |
| URI: | http://repository.its.ac.id/id/eprint/138042 |
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