Pengaruh Melatonin Terhadap Pertumbuhan Dan Profil Metabolit Pada Tanaman Keji Beling (Strobilanthes Crispus) Secara In Vitro

Amelia, Rikke (2026) Pengaruh Melatonin Terhadap Pertumbuhan Dan Profil Metabolit Pada Tanaman Keji Beling (Strobilanthes Crispus) Secara In Vitro. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Strobilanthes crispus (keji beling), merupakan tanaman obat yang memiliki berbagai aktivitas farmakologis, seperti antioksidan, antidiabetes, antikanker, antitumor, antibakteri, dan sitotoksik. Ketersediaan tanaman ini masih terbatas karena perbanyakannya umumnya dilakukan secara vegetatif melalui stek. Penelitian ini bertujuan untuk menganalisis pengaruh melatonin sebagai elisitor terhadap pertumbuhan dan profil metabolit S. crispus secara in vitro. Penelitian menggunakan eksplan nodus steril yang ditanam pada media Murashige and Skoog (MS) dengan penambahan 0,25 ppm NAA dan 3 ppm BAP sebagai zat pengatur tumbuh serta melatonin pada konsentrasi 0 μM, 25 μM, 50 μM, 75 μM, dan 100 μM. Penelitian disusun menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas lima perlakuan dengan lima ulangan. Parameter yang diamati meliputi persentase organogenesis, persentase kalogenesis, morfologi kalus, tinggi planlet, berat basah, jumlah tunas, jumlah nodus, jumlah akar, dan jumlah daun. Profil metabolit dianalisis menggunakan metode Gas Chromatography–Mass Spectrometry (GC-MS). Hasil penelitian menunjukkan bahwa seluruh perlakuan menghasilkan pembentukan kalus dan tunas dengan persentase 100%, namun tidak menghasilkan akar. Perlakuan melatonin 100 µM memberikan respons terbaik dengan mempercepat organogenesis hingga 3,7 HST serta menghasilkan berat basah total dan berat kalus tertinggi. Pemberian melatonin juga mengubah morfologi kalus yang ditandai dengan perubahan warna dari cokelat tua gelap hingga menjadi krem serta perubahan tekstur dari kompak menjadi friable. Analisis GC-MS mendeteksi 25 senyawa yang tergolong dalam kelompok asam lemak, karbohidrat, terpenoid, sterol dan fenolik. Pemberian melatonin memengaruhi komposisi metabolit dengan menurunkan dominansi asam lemak serta meningkatkan proporsi karbohidrat dan terpenoid pada konsentrasi tertentu. Hasil penelitian menunjukkan bahwa melatonin berpotensi meningkatkan respons morfogenesis sekaligus memodulasi profil metabolit S. crispus secara in vitro.
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Strobilanthes crispus (keji beling) is a medicinal plant that exhibits various pharmacological activities, including antioxidant, antidiabetic, anticancer, antitumor, antibacterial, and cytotoxic properties. However, its availability remains limited because propagation is generally carried out vegetatively through stem cuttings. This study aimed to evaluate the effect of melatonin as an elicitor on the growth and metabolite profile of S. crispus cultured in vitro. Sterile nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.25 ppm NAA, 3 ppm BAP as plant growth regulators, and melatonin at concentrations of 0, 25, 50, 75, and 100 μM. The experiment was arranged in a Completely Randomized Design (CRD) consisting of five treatments with five replications. The observed parameters included the percentage of organogenesis, percentage of callogenesis, callus morphology, plantlet height, fresh weight, number of shoots, number of nodes, number of roots, and number of leaves. Metabolite profiling was performed using Gas Chromatography–Mass Spectrometry (GC-MS). The results showed that all treatments achieved 100% callus and shoot formation, whereas no root formation was observed. Melatonin at 100 μM produced the best response by accelerating organogenesis to 3.7 days after treatment and resulting in the highest total fresh weight and callus weight. Melatonin application also altered callus morphology, as indicated by changes in color from dark brown to cream and in texture from compact to friable. GC-MS analysis detected 25 compounds belonging to the fatty acid, carbohydrate, terpenoid, sterol, and phenolic groups. Melatonin treatment modified the metabolite composition by reducing the predominance of fatty acids while increasing the proportion of carbohydrates and terpenoids at specific concentrations. Overall, melatonin has the potential to enhance morphogenetic responses while modulating the metabolite profile of S. crispus cultured in vitro.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Elisitasi; Gas Chromatography-Mass Spectrometry; Kultur in Vitro; Profil Metabolit; Strobilanthes crispus. ======================================================================================================================== Elicitation; Gas Chromatography–Mass Spectrometry; In Vitro Culture; Metabolite Profiling; Strobilanthes crispus.
Subjects:	Q Science > QK Botany S Agriculture > SB Plant culture S Agriculture > SB Plant culture > SB409.58 Plant propagation. Including in vitro propagation
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Rikke Amelia
Date Deposited:	27 Jul 2026 06:00
Last Modified:	27 Jul 2026 06:00
URI:	http://repository.its.ac.id/id/eprint/138216

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