Najah, Cindy Fatihatun Najah (2026) Pengaruh Variasi Suhu Dan Salinitas Terhadap Kemampuan Biodegradasi Plastik Low-Density Polyethylene (LDPE) Oleh Konsorsium Isolat BIO-B (Brevibacillus Formosus) Dan BIO-G (Stenotrophomonas Maltophilia). Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Low-Density Polyethylene (LDPE) merupakan salah satu jenis plastik yang sulit terdegradasi secara alami sehingga berkontribusi terhadap pencemaran lingkungan. Biodegradasi menggunakan bakteri menjadi salah satu alternatif yang ramah lingkungan, namun efektivitasnya dipengaruhi oleh faktor lingkungan, seperti suhu dan salinitas. Penelitian ini bertujuan untuk mengetahui pengaruh variasi suhu dan salinitas terhadap kemampuan isolat BIO-B (Brevibacillus formosus) dan BIO-G (Stenotrophomonas maltophilia) dalam mendegradasi plastik LDPE. Penelitian dilakukan selama 90 hari menggunakan Rancangan Acak Kelompok (RAK) dengan variasi suhu 30°C, 37°C, dan 45°C serta salinitas 3‰, 15‰, dan 30‰ dengan tiga ulangan. Parameter yang diamati meliputi persentase kehilangan berat kering LDPE, perubahan gugus fungsi menggunakan Fourier Transform Infrared (FTIR), serta viabilitas bakteri setelah inkubasi. Data kehilangan berat kering dianalisis menggunakan uji Two-Way Analysis of Variance (ANOVA). Hasil analisis pada bulan ketiga menunjukkan bahwa faktor suhu berpengaruh signifikan terhadap kehilangan berat kering LDPE (p < 0,05), sedangkan faktor salinitas maupun interaksi antara suhu dan salinitas tidak berpengaruh signifikan (p > 0,05). Namun, uji lanjut Tukey menunjukkan bahwa tidak terdapat perbedaan nyata antar variasi suhu. Persentase biodegradasi tertinggi sebesar 5,30% diperoleh pada perlakuan suhu 30°C dan salinitas 15‰ pada bulan ketiga. Analisis FTIR menunjukkan munculnya peak baru pada daerah 3200–3600 cm⁻¹ pada perlakuan suhu 30°C dan salinitas 15‰ setelah tiga bulan inkubasi, yang mengindikasikan terbentuknya gugus hidroksil selama proses biodegradasi. Selain itu, hasil uji viabilitas menunjukkan bahwa konsorsium BIO-B dan BIO-G tetap hidup setelah masa inkubasi selama 90 hari. Hasil penelitian ini menunjukkan bahwa konsorsium BIO-B dan BIO-G mampu mendegradasi plastik LDPE pada seluruh variasi suhu dan salinitas yang diuji.
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Low-Density Polyethylene (LDPE) is one of the most widely used plastics and is highly resistant to natural degradation, contributing to environmental pollution. Bacterial biodegradation has emerged as an environmentally friendly alternative for reducing plastic waste. However, its effectiveness is influenced by environmental factors such as temperature and salinity. This study aimed to investigate the effects of temperature and salinity variations on the biodegradation of LDPE by BIO-B (Brevibacillus formosus) and BIO-G (Stenotrophomonas maltophilia) isolates. The experiment was conducted over 90 days using a Randomized Complete Block Design (RCBD) with three temperature levels (30°C, 37°C, and 45°C) and three salinity levels (3‰, 15‰, and 30‰), each with three replications. The observed parameters included the percentage of LDPE dry weight loss, changes in functional groups analyzed by Fourier Transform Infrared (FTIR) spectroscopy, and bacterial viability after incubation. Dry weight loss data were analyzed using a two-way analysis of variance (ANOVA). The results obtained in the third month showed that temperature had a significant effect on LDPE dry weight loss (p < 0.05), whereas salinity and the interaction between temperature and salinity had no significant effect (p > 0.05). However, Tukey's post hoc test revealed no significant differences among the temperature treatments. The highest biodegradation percentage (5.30%) was obtained at 30°C and 15‰ salinity in the third month. FTIR analysis revealed the appearance of a new absorption peak in the 3200–3600 cm⁻¹ region in the 30°C and 15‰ salinity treatment after three months of incubation, indicating the formation of hydroxyl groups during the biodegradation process. In addition, the bacterial viability test demonstrated that the BIO-B and BIO-G consortium remained viable after 90 days of incubation. These findings indicate that the BIO-B and BIO-G consortium was capable of degrading LDPE under all tested temperature and salinity conditions.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biodegradasi, BIO-B, BIO-G, LDPE, Plastik, Salinitas, Suhu, Biodegradation, BIO-B, BIO-G, Plastic, Salinity, Temperature. |
| Subjects: | Q Science Q Science > QH Biology > QH301 Biology Q Science > QP Physiology > QP517.B5 Biodegradation. Q Science > QP Physiology > QP801.P64 Polymers--Biodegradation Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Cindy Fatihatun Najah |
| Date Deposited: | 28 Jul 2026 03:04 |
| Last Modified: | 28 Jul 2026 03:04 |
| URI: | http://repository.its.ac.id/id/eprint/138442 |
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