KEANEKARAGAMAN DAN POTENSI KAPANG PELARUT FOSFAT DARI PULAU POTERAN, SUMENEP, MADURA

AKBAR, JEAN ISHENDY PUTERA (2014) KEANEKARAGAMAN DAN POTENSI KAPANG PELARUT FOSFAT DARI PULAU POTERAN, SUMENEP, MADURA. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pulau Poteran, Sumenep, Madura memiliki tanah
berbahan induk batu kapur dan mediteran merah, menyebabkan
tanah cenderung basa, sehingga unsur fosfor terikat oleh unsur
Ca. Salah satu kelompok mikroorganisme yang mampu
mengubahnya adalah kapang. Penelitian ini dilakukan untuk
mengetahui genus kapang pelarut fosfat dari Pulau Poteran dan
potensinya menekan pertumbuhan kapang patogen tanah secara
in vitro tanpa menyebabkan infeksi pada tembakau.
Isolasi dilakukan di lima titik berbeda, lalu diinkubasi di
Laboratorium Biologi ITS. Isolat murni diuji dalam medium
selektif Pikovskaya. Selanjutnya, dilakukan dua uji potensi, yaitu
uji antagonis terhadap kapang patogen F. oxysporum, serta uji
patogenitas terhadap daun tembakau varietas Prancak 95.
Hasil menunjukkan, hanya empat isolat (A, B, C, D) yang
mampu membentuk zona bening, diduga mendekati genus
Aspergillus, dengan rerata indeks kelarutan fosfat (IKF) tiap
isolat: A=0,15; B=0,13; C=0,06; D=0,08. Konsentrasi Ptersedia
yang dihasilkan tiap isolat: A=9,27; B=10,24; C=6,09;
D=4,21 ppm. Seluruh isolat diduga memiliki sifat antagonis,
dengan nilai penghambatan (P) untuk isolat A=0,5065; B=0,435;
C=0,6305; D=0,5393. Isolat A dan B diduga memiliki sifat
patogen, sedangkan isolat C dan D tidak menunjukkan gejala
patogen pada daun tembakau.

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Poteran Island, Sumenep, Madura has made from
limestone soil and red mediterranean soil, causing tend to be
alkaline, so the phosphorus element bound by Ca unsure. One
group of microorganisms that are able to change it is mold. The
aim of this research is to know the genera of phosphate
solubilizing molds from Poteran Island and the potency to
suppress of soil pathogeny mold without causing infection to
tobacco under in vitro condition.
Isolation conducted in five different spot, then incubated
in the laboratory of Biology ITS. Pure isolate tested by selective
medium of Pikovskaya. Then, conducted with two potency tests,
the antagonistic test toward pathogeny mold F. oxysporum, and
patogenicity test toward tobacco leaf Prancak 95 var.
The result shows, only four isolate (A, B, C, D) that
appeared the halozone form, estimated to Aspergillus genera,
with the mean of soluble phosphorus index (SPI) of each isolate:
A=0,15; B=0,13; C=0,06; D=0,08. P-available concentration that
resulted from each isolate: A=9,27; B=10,24; C=6,09; D=4,21
ppm. All isolates estimated have an antagonistic disposition with
inhibitory (I) value for isolate A=0,5065; B=0,435; C=0,6305;
D=0,5393. Isolate A and B are estimated pathogen, but isolate C
and D are didn’t shows any causes on tobacco leaf.

Item Type:	Thesis (Undergraduate)
Additional Information:	RSBi 571.98 Akb k
Uncontrolled Keywords:	fosfat, kapang, Pulau Poteran, antagonis, patogenitas.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Anis Wulandari
Date Deposited:	09 Jan 2017 04:22
Last Modified:	09 Jan 2017 04:22
URI:	http://repository.its.ac.id/id/eprint/1401

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