KEANEKARAGAMAN DAN POTENSI KAPANG PELARUT FOSFAT DARI PULAU POTERAN, SUMENEP, MADURA

AKBAR, JEAN ISHENDY PUTERA (2014) KEANEKARAGAMAN DAN POTENSI KAPANG PELARUT FOSFAT DARI PULAU POTERAN, SUMENEP, MADURA. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pulau Poteran, Sumenep, Madura memiliki tanah berbahan induk batu kapur dan mediteran merah, menyebabkan tanah cenderung basa, sehingga unsur fosfor terikat oleh unsur Ca. Salah satu kelompok mikroorganisme yang mampu mengubahnya adalah kapang. Penelitian ini dilakukan untuk mengetahui genus kapang pelarut fosfat dari Pulau Poteran dan potensinya menekan pertumbuhan kapang patogen tanah secara in vitro tanpa menyebabkan infeksi pada tembakau. Isolasi dilakukan di lima titik berbeda, lalu diinkubasi di Laboratorium Biologi ITS. Isolat murni diuji dalam medium selektif Pikovskaya. Selanjutnya, dilakukan dua uji potensi, yaitu uji antagonis terhadap kapang patogen F. oxysporum, serta uji patogenitas terhadap daun tembakau varietas Prancak 95. Hasil menunjukkan, hanya empat isolat (A, B, C, D) yang mampu membentuk zona bening, diduga mendekati genus Aspergillus, dengan rerata indeks kelarutan fosfat (IKF) tiap isolat: A=0,15; B=0,13; C=0,06; D=0,08. Konsentrasi Ptersedia yang dihasilkan tiap isolat: A=9,27; B=10,24; C=6,09; D=4,21 ppm. Seluruh isolat diduga memiliki sifat antagonis, dengan nilai penghambatan (P) untuk isolat A=0,5065; B=0,435; C=0,6305; D=0,5393. Isolat A dan B diduga memiliki sifat patogen, sedangkan isolat C dan D tidak menunjukkan gejala patogen pada daun tembakau. ============================================================ Poteran Island, Sumenep, Madura has made from limestone soil and red mediterranean soil, causing tend to be alkaline, so the phosphorus element bound by Ca unsure. One group of microorganisms that are able to change it is mold. The aim of this research is to know the genera of phosphate solubilizing molds from Poteran Island and the potency to suppress of soil pathogeny mold without causing infection to tobacco under in vitro condition. Isolation conducted in five different spot, then incubated in the laboratory of Biology ITS. Pure isolate tested by selective medium of Pikovskaya. Then, conducted with two potency tests, the antagonistic test toward pathogeny mold F. oxysporum, and patogenicity test toward tobacco leaf Prancak 95 var. The result shows, only four isolate (A, B, C, D) that appeared the halozone form, estimated to Aspergillus genera, with the mean of soluble phosphorus index (SPI) of each isolate: A=0,15; B=0,13; C=0,06; D=0,08. P-available concentration that resulted from each isolate: A=9,27; B=10,24; C=6,09; D=4,21 ppm. All isolates estimated have an antagonistic disposition with inhibitory (I) value for isolate A=0,5065; B=0,435; C=0,6305; D=0,5393. Isolate A and B are estimated pathogen, but isolate C and D are didn’t shows any causes on tobacco leaf.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 571.98 Akb k
Uncontrolled Keywords: fosfat, kapang, Pulau Poteran, antagonis, patogenitas.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Mathematics and Science > Biology > (S1) Undergraduate Theses
Depositing User: Mrs Anis Wulandari
Date Deposited: 09 Jan 2017 04:22
Last Modified: 09 Jan 2017 04:22
URI: http://repository.its.ac.id/id/eprint/1401

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