Kajian Profil Asam Lemak Mikroalga Dalam Media Pertumbuhan Dengan Penambahan Amonia Sebagai Kandidat Bioremediator Dalam Sistem Akuaponik

Ula, Najwa Aufa Rahmatana Al Ula (2026) Kajian Profil Asam Lemak Mikroalga Dalam Media Pertumbuhan Dengan Penambahan Amonia Sebagai Kandidat Bioremediator Dalam Sistem Akuaponik. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sistem akuaponik memiliki tantangan berupa akumulasi amonia (NH₃) yang bersifat toksik bagi ikan. Mikroalga merupakan solusi fitoremediasi yang adaptif dengan kemampuan mengasimilasi amonia langsung sebagai sumber nitrogen untuk pertumbuhan dan sintesis biomolekul bernilai tinggi seperti asam lemak. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan amonia terhadap profil asam lemak dan kapasitas bioremediasi pada H. pluvialis, C. vulgaris, dan S. platensis. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) satu faktor dengan empat tingkat konsentrasi amonia (kontrol, 10 ppm, 20 ppm, dan 30 ppm). Parameter yang diamati meliputi kepadatan sel, biomassa kering, serta analisis profil asam lemak menggunakan GC-MS. Hasil penelitian menunjukkan bahwa respon terbaik pada setiap parameter bervariasi antar spesies bergantung pada perlakuan yang diberikan. Pertumbuhan sel dan akumulasi biomassa kering tertinggi pada ketiga spesies diperoleh pada perlakuan kontrol. S. platensis menghasilkan kepadatan sel tertinggi sebesar 460.000 sel/mL, biomassa kering tertinggi sebesar 18,67 mg/L, serta total asam lemak tertinggi sebesar 7,246% pada perlakuan kontrol. Adapun efisiensi bioremediasi tertinggi S. platensis dicapai pada perlakuan 10 ppm sebesar 82,30%. H. pluvialis menghasilkan kepadatan sel tertinggi sebesar 600.000 sel/mL, biomassa kering tertinggi sebesar 20 mg/L, serta total asam lemak tertinggi sebesar 53,582% pada perlakuan kontrol. Efisiensi bioremediasi tertinggi H. pluvialis diperoleh pada perlakuan 10 ppm sebesar 85,68%. Sementara itu, C. vulgaris menghasilkan kepadatan sel tertinggi sebesar 430.000 sel/mL dan biomassa kering tertinggi sebesar 14 mg/L pada perlakuan kontrol, namun total asam lemak tertinggi diperoleh pada perlakuan amonia 30 ppm sebesar 15,457% serta efisiensi bioremediasi tertinggi sebesar 66,30%.
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Aquaponic systems face the challenge of ammonia (NH₃) accumulation, which is toxic to fish. Microalgae offer an adaptive phytoremediation solution, as they can directly assimilate ammonia as a nitrogen source for growth and the synthesis of high-value biomolecules such as fatty acids. This study aims to determine the effect of ammonia addition on the fatty acid profile and bioremediation capacity of H. pluvialis, C. vulgaris, and S. platensis. The research method employed a one-factor completely randomized design (CRD) with four levels of ammonia concentration (control, 10 ppm, 20 ppm, and 30 ppm). The parameters observed included cell density, dry biomass, and fatty acid profile analysis using GC-MS. The results of the study showed that the optimal response for each parameter varied among species depending on the treatment applied. The highest cell growth and dry biomass accumulation for all three species were obtained in the control treatment. S. platensis produced the highest cell density of 460,000 cells/mL, the highest dry biomass of 18.67 mg/L, and the highest total fatty acid content of 7.246% in the control treatment. The highest bioremediation efficiency for S. platensis was achieved in the 10 ppm treatment at 82.30%. H. pluvialis produced the highest cell density of 600,000 cells/mL, the highest dry biomass of 20 mg/L, and the highest total fatty acid content of 53.582% in the control treatment. The highest bioremediation efficiency of H. pluvialis was obtained at the 10 ppm treatment level, at 85.68%. Meanwhile, C. vulgaris produced the highest cell density of 430,000 cells/mL and the highest dry biomass of 14 mg/L in the control treatment, however, the highest total fatty acid content was obtained in the 30 ppm ammonia treatment at 15.457%, along with the highest bioremediation efficiency of 66.30%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Asam Lemak, Amonia, Chlorella vulgaris, Haematococcus pluvialis, Mikroalga, Spirulina platensis,Fatty Acid, Amonia, Chlorella vulgaris, Haematococcus pluvialis, Microalgae, Spirullina platensis.
Subjects:	Q Science > QK Botany > QK568 Microalgae
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Najwa Aufa Rahmatana Al Ula
Date Deposited:	31 Jul 2026 00:41
Last Modified:	31 Jul 2026 00:41
URI:	http://repository.its.ac.id/id/eprint/140300

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