Sintesis Hidrogel Dengan Penambahan AgNPs-Bromelain sebagai Antibacterial Agent Untuk Aplikasi Wound Dressing

Ahmadina, Bareerah and Arofah, Mamlu'atul Husna (2026) Sintesis Hidrogel Dengan Penambahan AgNPs-Bromelain sebagai Antibacterial Agent Untuk Aplikasi Wound Dressing. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pengembangan wound dressing yang efektif dan aman menjadi tantangan penting dalam penanganan luka akibat risiko infeksi dan keterbatasan dressing konvensional. Penelitian ini mengembangkan hydrogel penambahan AgNPs-Bromelain sebagai antibacterial agent untuk aplikasi wound dressing. Sintesis hydrogel berbasis PVA/CMC dengan crosslinking citric acid yang divariasikan konsentrasinya untuk mengetahui pengaruhnya terhadap matriks hydrogel yang dihasilkan, serta mengetahui karakteristik AgNPsBromelain yang ditambahkan dalam hydrogel dan hydrogel berhasil disintesis dnegan karakterisasi menggunakan FTIR, uji swelling, uji tensile, antibakteri, dan in vivo. Hasil FTIR menunjukkan bahwa perubahan kadar CA memengaruhi struktur kimia hydrogel dengan mengubah bentuk pita O-H, C=O, dan C-O/C-O-C. AgNPs-Bromelain terdeteksi berdasarkan perubahan pada pita protein bromelain yang khas, serta didukung oleh pergeseran puncak SPR pada data UV-Vis, yang menunjukkan adanya interaksi antara bromelain dengan permukaan AgNPs sebagai capping agent. Hasil swelling menunjukkan bahwa hydrogel PVA/CMC/CA/AgNPs-Bromelain dengan CA 0,5% memiliki swelling tertinggi sebesar 658,07%% pada 240 menit dan tetap stabil selama perendaman. Hasil tensile menunjukkan bahwa penambahan AgNPs-Bromelain menurunkan tensile strength dari rentang 7,853-33,927 MPa menjadi 4,022-18,960 MPa, tetapi CA 1,0% menunjukkan penurunan paling kecil, yaitu dari 7,853 MPa menjadi 4,022 MPa. Pengaruh konsentrasi CA bersifat spesifik terhadap parameter pengujian, dengan karakteristik FTIR menunjukkan ikatan ikatan silang paling jelas pada CA 0,6%, kemampuan swelling tertinggi pada CA 0,5%, tensile terbaik pada CA 0,7%, dan aktivitas antibakteri pada CA 1,0%. Perbedaan tersebut menunjukkan bahwa konsentrasi CA memberikan pengaruh yang berbeda terhadap setiap karakteristik hidrogel, sehingga formulasi optimum perlu ditentukan berdasarkan kebutuhan utama wound dressing.
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The development of effective and safe wound dressing is a significant challenge in wound management due to the risk of infection and the limitations of conventional dressings. This study developed a hydrogel incorporating AgNPs and bromelain as antibacterial agents for use in wound dressings. PVA/CMC-based hydrogels were syhnthesized using citric acid crosslinking at varying concentration to determine its effect on the resulting hydrogel matrix, as well as to characterize the AgNPs-Bromelain added to the hydrogel. The hydrogels were successfully synthesized and characterized using FTIR, swelling tests. Tensile test, and in vivo testing. The FTIR analysis showed that CA content affected the hydrogel chemical structure through changes in the O-H, C=O, and C-O/C-O-C bands. AgNPs-Bromelain was identified from shifts in characteristic bromelain protein bands and the SPR peak in the UV-Vis data, indicating bromelain interaction with the AgNPs surface as a capping agent. The PVA/CMC/CA/AgNPs-Bromelain hydrogel with CA 0,5% showed the highest swelling of 658,07% at 240 minutes and remained stable during immersion. Tensile results showed that AgNPs-Bromelain reduced tensile strength from 7,853-33,927 MPa to 4,022-18,960 MPa, but 1,0% showed the smallest decrease, from 7,853 MPa to 4,022 MPa. CA concentration affected each property differently, with the clearest FTIR evidence of cross-linking at 0.6%, the highest swelling at 0.5%, the best tensile strength at 0.7%, and the greatest antibacterial activity at 1.0%. Thus, the optimal CA concentration depends on the primary wound-dressing requirements.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	AgNPs, Aktivitas Antibakteri, Bromelain, Hydrogel, Wound Dressing, AgNPs, Antibacterial activity, Bromelain, Hydrogel, Wound dressing
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP1140 Polymers T Technology > TP Chemical technology > TP156.C64 Controlled release technology T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Vocational > 24305-Industrial Chemical Engineering Technology
Depositing User:	Mamluatul Husna Arofah
Date Deposited:	30 Jul 2026 08:53
Last Modified:	30 Jul 2026 08:53
URI:	http://repository.its.ac.id/id/eprint/140313

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