Rahmadhani, Nevinia (2026) Pengaruh Konsentrasi Aluminium Klorida (AlCl3) terhadap Pertumbuhan Angsana (Pterocarpus indicus) pada Kondisi Kultue in Vitro di Media Murashige and Skoog pH Rendah dan Deteksi Gen Tahan Asam. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Pterocarpus indicus merupakan spesies pohon bernilai ekologis dan ekonomis tinggi yang membutuhkan metode perbanyakan cepat untuk mendukung konservasi dan rehabilitasi lahan terdegradasi. Penelitian ini menilai pengaruh cekaman Aluminium Klorida (AlCl3) terhadap pertumbuhan eksplan P. indicus dalam kultur in vitro serta mendeteksi gen Aluminum Activated Malate Transporter (ALMT) dan Sensitive To Proton Rhizotoxicity 1 (STOP1) sebagai penanda toleransi asam. Eksplan tunas dikultur pada medium Murashige and Skoog (MS) dengan beberapa kombinasi Zat Pengatur Tumbuh yaitu indole-3- butyric acid (IBA), Benzyl Amino Purine (BAP), dan metaTopolin (mT), kemudian disubkultur pada medium perlakuan yang mengandung AlCl₃ dengan variasi 0, 100, 200, 300, dan 400 ppm untuk meniru kondisi tanah masam. Penelitian ini mengamati parameter pertumbuhan eksplan untuk menilai respons morfologi terhadap cekaman asam. Penelitian ini juga melakukan deteksi gen ALMT dan STOP1 melalui isolasi DNA eksplan, kemudian mengamplifikasinya dengan Polymerase Chain Reaction (PCR) dan memvisualisasikan produk PCR menggunakan elektroforesis. Hasil penelitian menunjukkan bahwa kombinasi ZPT tidak berpengaruh signifikan terhadap pertumbuhan eksplan, sedangkan konsentrasi AlCl₃ berpengaruh nyata terhadap pertumbuhan eksplan. Hasil uji PCR mengonfirmasi keberadaan gen ALMT dan STOP1 pada P. indicus, yang diduga berperan dalam mekanisme eksklusi aluminium melalui sekresi malat.
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Pterocarpus indicus is a tree species of high ecological and economic value that requires rapid propagation methods to support conservation and the rehabilitation of degraded land. This study evaluated the effect of aluminum chloride (AlCl₃) stress on the growth of P. indicus explants in in vitro culture and identified the Aluminum Activated Malate Transporter (ALMT) and Sensitive to Proton Rhizotoxicity 1 (STOP1) as markers of acid tolerance. Shoot explants were cultured on Murashige and Skoog (MS) medium with various combinations of plant growth regulators, namely indole-3-butyric acid (IBA), benzyl amino purine (BAP), and meta-topolin (mT), and then subcultured in treatment media containing AlCl₃ at concentrations of 0, 100, 200, 300, and 400 ppm to mimic acidic soil conditions. This study examined explant growth parameters to assess morphological responses to acid stress. The study also detected the ALMT and STOP1 genes by isolating DNA from the explants, amplifying it via Polymerase Chain Reaction (PCR), and visualizing the PCR products using electrophoresis. The results showed that the combination of plant growth regulators had no significant effect on explant growth, whereas the concentration of AlCl₃ had a significant effect on explant growth. The PCR test results confirmed the presence of the ALMT and STOP1 genes in P. indicus, which are believed toplay a role in the mechanism of aluminum exclusion through malate secretion.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Gen ALMT/STOP1, Kultur in vitro, pH rendah, Pterocarpus indicus. AlCl₃, ALMT/STOP1 gene, in vitro culture, low pH |
| Subjects: | Q Science > QH Biology > QH426 Genetics S Agriculture > SB Plant culture > SB409.58 Plant propagation. Including in vitro propagation |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Nevinia Rahmadhani |
| Date Deposited: | 31 Jul 2026 02:40 |
| Last Modified: | 31 Jul 2026 02:40 |
| URI: | http://repository.its.ac.id/id/eprint/140540 |
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