Skrining Nanokristal Dari Senyawa Fluoresen Untuk Deteksi Triptamin Berbasis Paper Analytical Device

Bella, Cristiana Sifa (2026) Skrining Nanokristal Dari Senyawa Fluoresen Untuk Deteksi Triptamin Berbasis Paper Analytical Device. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Triptamin merupakan monoamina berbasis indolin dari metabolisme triptofan yang terdapat dalam sistem biologis dan dapat terakumulasi pada pangan fermentasi, sehingga deteksinya penting untuk memantau risiko kesehatan. Penelitian ini mengembangkan microfluidic paper-based analytical device (µPAD) berbasis fluoresensi yang diintegrasikan dengan nanokristal organik untuk deteksi triptamin yang sederhana, cepat, dan sensitif. Empat senyawa organik hasil sintesis siap pakai, yaitu bis(quinolin-8-yloxy)zinc (F1), tris(quinolin-8-yloxy)aluminum (F2), 1,1'-([2,2’-binaphthalene]-8,8’-diyl)bis(2-(tert-butyl)urea) (F3), dan bis((2-(3-tert-butyl)ureido)quinolin-8-yl)oxy)zinc (F4) dipilih karena memiliki sifat hidrofobik tinggi dan kelarutan sangat rendah dalam fase air. Modifikasi menjadi bentuk nanokristal terdispersi melalui metode represipitasi dilakukan agar material sensor dapat diaplikasikan pada kertas µPAD. Hasil skrining menunjukkan bahwa F3, turunan bisurea berbasis binaftil yang memanfaatkan donor ikatan hidrogen dan stabilitas gugus tert-butyl, memiliki performa terbaik dengan stabilitas dispersi yang baik, ukuran partikel rata-rata 151 nm, dan indeks polidispersitas (PDI) sebesar 0,285. Karakterisasi Field Emission Scanning Electron Microscopy (FESEM) mengonfirmasi morfologi sferis dari nanokristal F3. Selanjutnya, nanokristal F3 diimobilisasi pada µPAD untuk mendeteksi triptamin pada rentang 0–20 mM. Pengukuran pada µPAD dilakukan menggunakan eksitasi lampu UV 365 nm, di mana hasil foto digitalnya dianalisis dengan perangkat lunak ImageJ untuk mengekstraksi parameter warna CIELAB (L*,a*, dan b*). Nilai ΔE menunjukkan hubungan linier terhadap konsentrasi triptamin dengan koefisien determinasi (R²) sebesar 0,9904. Secara keseluruhan, µPAD berbasis nanokristal F3 berpotensi besar sebagai platform deteksi optik yang efektif.
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Tryptamine is an indole-based monoamine derived from tryptophan metabolism that is naturally present in biological systems and can accumulate in fermented foods, making its detection important for monitoring potential health risks. This study developed a fluorescence-based microfluidic paper-based analytical device (µPAD) integrated with organic nanocrystals for the simple, rapid, and sensitive detection of tryptamine. Four ready-to-use synthesized organic compounds, namely bis(quinolin-8-yloxy)zinc (F1), tris(quinolin-8-yloxy)aluminum (F2), 1,1'-([2,2'-binaphthalene]-8,8'-diyl)bis(2-(tert-butyl)urea) (F3), and bis((2-(3-tert-butyl)ureido)quinolin-8-yl)oxy)zinc (F4), were selected because of their high hydrophobicity and extremely low solubility in aqueous media. These compounds were converted into dispersed nanocrystals using the reprecipitation method to enable their application as sensing materials on the µPAD. Screening results identified F3, a binaphthyl-based bisurea derivative that utilizes hydrogen-bond donor interactions and the stabilizing effect of tert-butyl groups, as the best-performing candidate, exhibiting good dispersion stability, an average particle size of 151 nm, and a polydispersity index (PDI) of 0.285. Field Emission Scanning Electron Microscopy (FESEM) characterization further confirmed the spherical morphology of the F3 nanocrystals. The F3 nanocrystals were subsequently immobilized on the µPAD for the detection of tryptamine over a concentration range of 0–20 mM. Measurements were performed under 365 nm UV excitation, and the resulting digital images were analyzed using ImageJ software to extract the CIELAB color parameters (L*, a*, and b*). The ΔE values exhibited a linear relationship with tryptamine concentration, with a coefficient of determination (R²) of 0.9904. Overall, the F3 nanocrystal-based µPAD demonstrates strong potential as an effective optical sensing platform for tryptamine detection.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Fluoresen; Microfluidic Paper-Based Analytical Device; Nanokristal; Triptamin Fluorescent; Microfluidic Paper-Based Analytical Device; Nanocrystal; Tryptamine
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD547 Flocculation, precipitation, adsorption, etc. Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Cristiana Sifa Bella
Date Deposited:	04 Aug 2026 04:35
Last Modified:	04 Aug 2026 04:35
URI:	http://repository.its.ac.id/id/eprint/140561

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