Peningkatan Efektivitas Senyawa Bioaktif Kulit Manggis Berbasis Nanopartikel Albumin Dengan Metode Microwave Assisted Synthesis Sebagai Antikanker

Putri, Sekar Langit Widya (2026) Peningkatan Efektivitas Senyawa Bioaktif Kulit Manggis Berbasis Nanopartikel Albumin Dengan Metode Microwave Assisted Synthesis Sebagai Antikanker. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

α-Mangostin merupakan senyawa bioaktif golongan xanthone dari kulit manggis (Garcinia mangostana L.) yang memiliki aktivitas antikanker, namun pemanfaatannya masih terbatas akibat bioavailabilitas yang rendah. Penelitian ini bertujuan mengembangkan pembuatan nanopartikel albumin berbasis α-mangostin menggunakan metode Microwave Assisted Synthesis sebagai sistem penghantaran obat untuk meningkatkan bioavailabilitas dan efektivitas antikanker. Kulit manggis diekstrak menggunakan metode Microwave Assisted Extraction (MAE) dan dilanjutkan dengna Batchwise Solvent Extraction (BSE), kemudian diformulasikan menjadi nanopartikel albumin. Optimasi dilakukan menggunakan metode Taguchi L9 dengan variasi rasio ekstrak terhadap Bovine Serum Albumin (BSA), daya microwave, dan waktu sintesis. Hasil penelitian menunjukkan bahwa kombinasi MAE-BSE meningkatkan kadar α-mangostin dari 239,03 menjadi 650,3 mg/g sampel dan total fenolik dari 643,995 menjadi 975,750 mg GAE/g ekstrak. Kondisi optimum diperoleh pada rasio ekstrak terhadap BSA 1%, daya microwave 450 W, dan waktu sintesis 120 detik, menghasilkan nanopartikel berukuran 49 ± 0,71 nm, indeks polidispersitas (PI) 0,213 ± 0,0009, zeta potential −33,58 ± 0,82 mV, efisiensi penjerapan (EE) 73,11%, dan kapasitas pemuatan (LC) 0,55%. Nanopartikel albumin menunjukkan aktivitas sitotoksik lebih tinggi terhadap sel MDA-MB-231 dengan nilai IC₅₀ 2,103 µg/mL dibandingkan ekstrak MAE-BSE (14,28 µg/mL). Hasil ini menunjukkan bahwa nanopartikel albumin berbasis α-mangostin berpotensi sebagai sistem penghantaran obat antikanker.
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α-Mangostin, a xanthone compound isolated from mangosteen (Garcinia mangostana L.) pericarp, possesses promising anticancer activity. However, its therapeutic application is limited by poor bioavailability. This study aimed to develop α-mangostin loaded albumin nanoparticles using Microwave Assisted Synthesis as a drug delivery system to improve bioavailability and anticancer efficacy. Mangosteen pericarp was extracted using Microwave Assisted Extraction (MAE) followed by Batchwise Solvent Extraction (BSE) and subsequently formulated into albumin nanoparticles. The synthesis process was optimized using the Taguchi L9 design by varying the extract to Bovine Serum Albumin (BSA) ratio, microwave power, and synthesis time. The combined MAE-BSE process increased the α-mangostin content from 248.75 to 705.6 mg/g sample and the total phenolic content from 643.995 to 975.750 mg GAE/g extract. The optimum formulation was obtained at an extract to BSA ratio of 1%, microwave power of 450 W, and synthesis time of 120 s, producing nanoparticles with a particle size of 49 ± 0.71 nm, a polydispersity index (PI) of 0.213 ± 0.0009, a zeta potential of −33.58 ± 0.82 mV, an entrapment efficiency (EE) of 73.11%, and a loading capacity (LC) of 0.55%. The albumin nanoparticles exhibited greater cytotoxic activity against MDA-MB-231 cells, with an IC₅₀ value of 2.103 µg/mL, compared with 14.28 µg/mL for the MAE-BSE extract. These findings demonstrate that α-mangostin-loaded albumin nanoparticles have potential as an effective anticancer drug delivery system.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Alpha Mangostin, Antikanker, Kulit Manggis, Microwave Assisted Synthesis, Nanopartikel Albumin, Alpha Mangostin, Anticancer, Mangosteen Pericarp, Microwave Assisted Synthesis, Nanoparticle Albumin
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24101-(S2) Master Thesis
Depositing User:	Sekar Langit Widya Putri
Date Deposited:	31 Jul 2026 06:10
Last Modified:	31 Jul 2026 06:10
URI:	http://repository.its.ac.id/id/eprint/140687

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