Dianah, Aisyah Nur (2026) Optimasi Biodekolorisasi Metil Oranye Oleh Pseudomonas Aeruginosa Terimobilisasi Pada Matriks Natrium Alginat/Polivinil Alkohol/Karbon Aktif Dengan Response Surface Methodology (Rsm). Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Pencemaran lingkungan akibat limbah pewarna tekstil, terutama metil oranye (MO), menjadi masalah serius karena karakteristiknya yang beracun, bersifat karsinogenik, dan sulit mengalami degradasi secara alami. Penelitian ini bertujuan untuk mengoptimalkan proses biodekolorisasi MO menggunakan Pseudomonas aeruginosa yang diimobilisasi dalam matriks SA/PVA/karbon aktif melalui pendekatan Response Surface Methodology (RSM). Desain eksperimental yang diterapkan adalah Box-Behnken Design (BBD) dengan tiga variabel bebas, yaitu pH larutan MO (4, 7, dan 10), suhu inkubasi (30; 37,5; dan 45 °C), dan waktu inkubasi (60, 210, dan 360 menit), sedangkan respons yang diukur adalah persentase dekolorisasi MO. Nilai Lack of Fit (LOF) pada model kuadratik dalam Analysis of Variance (ANOVA) menghasilkan F sebesar 2,95 dan P sebesar 0,2634, yang melebihi ambang batas 0,05, sehingga LOF dinyatakan tidak signifikan. Berdasarkan solusi optimasi RSM, kondisi terbaik yang diperoleh adalah pH 7, suhu 39 °C, dan waktu inkubasi selama 259 menit dengan efisiensi biodekolorisasi sebesar 52,47%. Temuan ini mengindikasikan bahwa sistem imobilisasi P. aeruginosa dalam matriks SA/PVA/karbon aktif merupakan pendekatan yang efektif dan menjanjikan dalam mendukung proses biodekolorisasi pewarna azo MO dari air limbah industri tekstil.
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Environmental pollution caused by textile dye wastewater, particularly methyl orange (MO), has become a serious concern due to its toxic, carcinogenic, and recalcitrant nature. This study aimed to optimize the biodecolorization process of MO using Pseudomonas aeruginosa immobilized in an SA/PVA/activated carbon matrix through a Response Surface Methodology (RSM) approach. The experimental design employed was the Box-Behnken Design (BBD) with three independent variables, namely pH of the MO solution (4, 7, and 10), incubation temperature (30, 37.5, and 45 °C), and incubation time (60, 210, and 360 minutes), while the measured response was the percentage of MO decolorization. The quadratic model obtained from Analysis of Variance (ANOVA) yielded a Lack of Fit (LOF) F-value of 2.95 and a P-value of 0.2634, which exceeded the 0.05 threshold, indicating that the LOF was not significant and the model was adequate. Based on the RSM optimization solution, the optimal conditions obtained were pH 7, temperature 39 °C, and an incubation time of 259 minutes, achieving a biodecolorization efficiency of 52.47%. These findings indicate that the immobilization system of P. aeruginosa in the SA/PVA/activated carbon matrix represents an effective and promising approach for supporting the biodecolorization of MO azo dye from textile industrial wastewater.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biodekolorisasi, Imobilisasi, Pseudomonas aeruginosa, RSM, Metil Oranye, Biodecolorization, Immobilization, Pseudomonas aeruginosa, RSM, Methyl Orange |
| Subjects: | Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Aisyah Nur Dianah |
| Date Deposited: | 31 Jul 2026 07:27 |
| Last Modified: | 31 Jul 2026 07:27 |
| URI: | http://repository.its.ac.id/id/eprint/140834 |
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