Optimasi Biodekolorisasi Metil Oranye oleh Bacillus subtilis Terimobilisasi pada Matriks SA/PVA/Karbon Aktif dengan Response Surface Methodology (RSM)

Wardani, Hanifah Sri Pramudya (2026) Optimasi Biodekolorisasi Metil Oranye oleh Bacillus subtilis Terimobilisasi pada Matriks SA/PVA/Karbon Aktif dengan Response Surface Methodology (RSM). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil oranye (MO) merupakan salah satu pewarna yang banyak digunakan dalam indutri tektil. Limbah MO bersifat toksik dan sulit terdegradasi secara alami, sehingga menyebabkan pencemaran lingkungan. Studi ini bertujuan untuk mengoptimasi proses biodekolorisiasi MO menggunakan Bacillus subtilis yang terimobilisasi pada matriks sodium alginat/polivinil alkohol/karbon aktif (SA/PVA/karbon aktif) melalui pendekatan response surface methodology (RSM). Optimasi dilakukan dengan menggunakan desain ekperimental Box-Behnken Design (BBD) dengan tiga variabel bebas, yaitu pH (4, 7, 10), suhu (30; 37,5 dan 45°C), dan waktu inkubasi (30, 165, dan 300 menit) serta respon yang dihasilkan berupa persentase dekolorisasi. Hasil optimasi RSM model kuadratik menunjukkan persentase dekolorisasi MO sebesar 62,58% dengan kondisi optimum pada pH 6,98 suhu 38,9°C dan waktu inkubasi 211 menit. Karakterisasi FTIR dan SEM dilakukan untuk mengidentifikasi gugus fungsi dan morfologi beads sebelum dan setelah dekolorisasi. Hasil karakterisasi tersebut menunjukkan bahwa telah terjadi proses adsorpsi pewarna MO pada beads. Optimasi RSM pada biodekolorisasi MO menggunakan bakteri B. subtilis terimobilisasi SA/PVA/karbon aktif terbukti efektif dan ramah lingkungan untuk pengolahan limbah zat warna MO.
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Methyl orange (MO) is one of the most widely used azo dyes in the textile industry. However, MO-containing wastewater is toxic and resistant to natural degradation, posing a significant threat to environmental quality.This study aimed to optimize the biodecolorization of MO using Bacillus subtilis immobilized in a sodium alginate/polyvinyl alcohol/activated carbon (SA/PVA/activated carbon) matrix through the application of response surface methodology (RSM). Optimization was performed using a Box-Behnken Design (BBD) with three independent variables: pH (4, 7, and 10), temperature (30, 37.5, and 45°C), and incubation time
(30, 165, and 300 min), while the response variable was the percentage of decolorization. The quadratic RSM model indicated that the optimum biodecolorization efficiency reached 62.58% under the conditions of pH 6.98, temperature 38.9°C, and incubation time 211 min. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analyses were conducted to investigate the functional groups and surface morphology of the immobilized
beads before and after the biodecolorization process. The characterization results confirmed the adsorption of methyl orange molecules onto the bead matrix after treatment. Overall, the RSM-optimized biodecolorization process using B. subtilis immobilized in the SA/PVA/activated carbon matrix demonstrated promising effectiveness as an environmentally friendly approach for the treatment of dye-containing wastewater.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi; Bacillus subtilis; Imobilisasi; Metil oranye; RSM, Biodecolorization; Bacillus subtilis; Immobilization; Methyl orange; RSM.
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Hanifah Sri Pramudya Wardani
Date Deposited:	31 Jul 2026 07:17
Last Modified:	31 Jul 2026 07:17
URI:	http://repository.its.ac.id/id/eprint/140837

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