Peningkatan Lipid dan PUFA pada Nannochloropsis oculata melalui Mutagenesis, Ko-Kultur, dan Manipulasi Spektrum Cahaya dalam Media POME

Pratiwi, Karenina (2026) Peningkatan Lipid dan PUFA pada Nannochloropsis oculata melalui Mutagenesis, Ko-Kultur, dan Manipulasi Spektrum Cahaya dalam Media POME. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Permintaan terhadap asam lemak tak jenuh ganda rantai panjang, khususnya eicosapentaenoic acid (EPA) dan docosahexaenoic acid (DHA), mendorong pengembangan sumber alternatif berbasis mikroalga. Nannochloropsis oculata dikenal mampu menghasilkan lipid bernilai tinggi, sedangkan Palm Oil Mill Effluent (POME) masih mengandung nutrien organik yang berpotensi dimanfaatkan sebagai medium kultivasi. Penelitian ini mengevaluasi pengaruh mutagenesis UV-C, ko-kultur dengan Bacillus subtilis, dan strategi spektrum cahaya dua tahap terhadap pertumbuhan serta profil asam lemak N. oculata dalam media POME. Strain wild type (WT) dan mutated strain (MT) dikultivasi selama tujuh hari pada kondisi aksenik dan ko-kultur. Kultur diberi cahaya putih, merah, atau kombinasi biru-hijau pada tahap pertama, kemudian seluruh perlakuan dialihkan ke cahaya merah pada hari ke-6 hingga hari ke-7. Pertumbuhan ditentukan berdasarkan jumlah sel, sedangkan komposisi asam lemak dianalisis sebagai fatty acid methyl esters (FAME) menggunakan GC-FID. Jumlah sel tertinggi diperoleh pada WT red coculture sebesar 55,20 juta sel/mL pada hari ke-7. Kandungan total PUFA tertinggi diperoleh pada axenic WT blue-green sebesar 51,83 ± 21,32% dari total FAME. EPA tertinggi dihasilkan oleh axenic WT white sebesar 10,00 ± 0,03%, sedangkan DHA tertinggi diperoleh pada axenic MT red sebesar 14,24 ± 0,01%. Hasil tersebut menunjukkan bahwa kondisi yang meningkatkan total PUFA tidak selalu menghasilkan EPA dan DHA tertinggi. Cahaya merah lebih mendukung pertumbuhan sel, ko-kultur cenderung meningkatkan pertumbuhan terutama pada WT, sedangkan mutagenesis mengubah profil asam lemak bergantung pada spektrum cahaya dan kondisi kultur. Temuan ini menunjukkan potensi integrasi mutagenesis, ko-kultur, manipulasi spektrum cahaya, dan pemanfaatan POME untuk menghasilkan biomassa N. oculata dengan profil lipid yang dapat diarahkan pada produksi PUFA, EPA, atau DHA.
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The growing demand for long-chain polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), has encouraged the development of alternative microalgal sources. Nannochloropsis oculata is recognized as a producer of high-value lipids, while Palm Oil Mill Effluent (POME) still contains organic nutrients that may support microalgal cultivation. This study evaluated the effects of UV-C mutagenesis, co-cultivation with Bacillus subtilis, and a two-stage light spectrum strategy on the growth and fatty acid profile of N. oculata cultivated in POME medium. Wild-type (WT) and mutated strains (MT) were cultivated for seven days under axenic and co-culture conditions. During the first stage, cultures were exposed to white, red, or blue-green light, followed by red-light exposure from day 6 to day 7. Growth was assessed based on cell density, while fatty acid composition was analyzed as fatty acid methyl esters (FAME) using GC-FID. The highest cell density was obtained in WT red coculture, reaching 55.20 million cells/mL on day 7. The highest total PUFA content was observed in axenic WT blue-green at 51.83 ± 21.32% of total FAME. The highest EPA content was obtained in axenic WT white at 10.00 ± 0.03%, whereas the highest DHA content was observed in axenic MT red at 14.24 ± 0.01%. These results indicate that an increase in total PUFA does not necessarily correspond to higher EPA and DHA levels. Red light generally supported cell growth, co-cultivation tended to enhance growth, particularly in WT, while mutagenesis altered the fatty acid profile depending on the light spectrum and cultivation condition. The findings highlight the potential integration of mutagenesis, co-cultivation, light spectrum manipulation, and POME utilization to produce N. oculata biomass with lipid profiles directed toward PUFA, EPA, or DHA production.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Nannochloropsis oculata, POME, mutagenesis, ko-kultur, PUFA, EPA Nannochloropsis oculata, POME, mutagenesis, co-culture, PUFA, EPA
Subjects:	Q Science Q Science > QK Botany > QK568 Microalgae
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering
Depositing User:	Karenina Anisya Pratiwi
Date Deposited:	01 Aug 2026 01:59
Last Modified:	01 Aug 2026 01:59
URI:	http://repository.its.ac.id/id/eprint/140954

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