Potensi Ekstrak Limbah Kulit Buah Kopi Robusta (Coffea canephora) Untuk Menghambat Pertumbuhan Aspergillus niger Dan Trichoderma harzianum

Rizqi, Alifia Virnanda (2026) Potensi Ekstrak Limbah Kulit Buah Kopi Robusta (Coffea canephora) Untuk Menghambat Pertumbuhan Aspergillus niger Dan Trichoderma harzianum. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Limbah kulit kopi robusta (Coffea canephora) merupakan salah satu limbah asal produksi kopi yang melimpah di Indonesia dan berpotensi dimanfaatkan karena memiliki sumber senyawa bioaktif, seperti kafein, tanin, flavonoid, saponin, dan polifenol yang diketahui memiliki aktivitas antimikroba. Tekstil mudah ditumbuhi jamur terutama pada kondisi lembap, sehingga rentan mengalami kerusakan struktur serat serta menimbulkan risiko kesehatan. Penggunaan antijamur sintetis dalam industri tekstil dapat menyebabkan dampak negatif terhadap lingkungan dan kesehatan, sehingga diperlukan alternatif bahan antijamur alami yang ramah lingkungan. Penelitian ini bertujuan untuk menguji potensi ekstrak limbah kopi robusta sebagai antijamur perusak tekstil, yaitu Aspergillus sp. dan Trichoderma sp. Ekstraksi dilakukan menggunakan metode maserasi yaitu pemisahan senyawa dengan cara perendaman dengan pelarut etanol 70%. Uji antijamur dilakukan melalui pengamatan index pertumbuhan pada media PDA dan penurunan biomassa pada media PDB. Hasil pengamatan pada media PDA menunjukkan bahwa peningkatan konsentrasi ekstrak dapat menurunkan laju pertumbuhan radial kedua jenis jamur. Efek penghambatan lebih dominan terhadap T. harzianum, yang ditandai dengan penghambatan pertumbuhan koloni dan sporulasi, sedangkan A. niger menunjukkan tingkat toleransi yang lebih tinggi. Namun, seluruh perlakuan tetap mencapai diameter pertumbuhan maksimum. Hasil tersebut menunjukkan bahwa ekstrak limbah kulit buah kopi robusta lebih bersifat fungistatik daripada fungisidal. Pada media PDB, pemberian ekstrak justru meningkatkan biomassa jamur. Dengan demikian, ekstrak limbah kulit buah kopi robusta dengan konsentrasi 5%-15% belum mampu menghambat pertumbuhan miselium secara optimal pada media cair maupun media padat.
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Robusta coffee husk waste (Coffea canephora) is one of the abundant coffee production wastes in Indonesia and has the potential to be utilized because it contains a source of bioactive compounds, such as caffeine, tannins, flavonoids, saponins, and polyphenols that are known to have antimicrobial activity. Textiles are easily overgrown with mold, especially in humid conditions, making them susceptible to damage to the fiber structure and posing health risks. The use of synthetic antifungals in the textile industry can have negative impacts on the environment and health, so alternative natural antifungal materials that are environmentally friendly are needed. This study aims to test the potential of robusta coffee waste extract as an antifungal agent for textile-destroying fungi, namely Aspergillus sp. and Trichoderma sp. Extraction was carried out using the maceration method, namely the separation of compounds by soaking in 70% ethanol solvent. The antifungal test was carried out by observing the growth index on PDA media and the reduction in biomass on PDB media. The results of observations on PDA media showed that increasing the extract concentration can reduce the radial growth rate of both types of fungi. The inhibitory effect was more dominant against T. harzianum, which was characterized by inhibition of colony growth and sporulation, while A. niger showed a higher tolerance level. However, all treatments still achieved maximum growth diameter. These results indicate that the extract of robusta coffee fruit peel waste is more fungistatic than fungicidal. In PDB media, the administration of the extract actually increased fungal biomass. Thus, robusta coffee fruit peel waste extract at a concentration of 5%-15% was not able to optimally inhibit mycelial growth in liquid or solid media.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	limbah kulit buah kopi robusta, antijamur alami, tekstil, maserasi, jamur perusak tekstil, robusta coffee fruit skin waste, natural antifungal, textiles, maceration, textile-damaging fungi
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Alifia Virnanda Rizqi
Date Deposited:	31 Jul 2026 01:20
Last Modified:	31 Jul 2026 01:20
URI:	http://repository.its.ac.id/id/eprint/140964

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