Pemanfaatan Limbah Cair Dan Padat Industri Agar Gracilaria Sp. Dan Produksi DHA Dengan Proses Kultivasi Heterotrof Thraustochytrids

Khatrunada, Cavela Arnetta and Rudianto, Anaral Al Ardhi (2026) Pemanfaatan Limbah Cair Dan Padat Industri Agar Gracilaria Sp. Dan Produksi DHA Dengan Proses Kultivasi Heterotrof Thraustochytrids. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Defisit global docosahexaenoic acid (DHA) yang diperkirakan melebihi satu juta ton per tahun menjadi permasalahan penting dalam ketahanan pangan akibat menurunnya stok ikan laut serta dampak lingkungan yang tidak berkelanjutan dari pemanfaatan minyak ikan konvensional. Thraustochytrids sebagai protista laut heterotrof memiliki kemampuan mengakumulasi lipid hingga sekitar 50% dari biomassa kering sehingga berpotensi menjadi sumber alternatif DHA. Namun, tingginya biaya substrat karbon komersial seperti glukosa masih menjadi kendala utama dalam produksi skala industri. Penelitian ini mengkaji pemanfaatan limbah industri agar dari Gracilaria sp. sebagai sumber karbon alternatif yang lebih ekonomis dan ramah lingkungan, mengingat sekitar 65–75% biomassa awal Gracilaria sp. umumnya menjadi limbah padat dan cair yang berpotensi menimbulkan pencemaran lingkungan. Limbah tersebut diolah melalui proses hidrolisis untuk mengonversi polisakarida kompleks menjadi gula sederhana yang dapat dimanfaatkan oleh Thraustochytrids sebagai sumber nutrisi. Hasil penelitian menunjukkan bahwa pemanfaatan limbah industri agar mampu mendukung pertumbuhan biomassa Thraustochytrids serta menghasilkan lipid kaya DHA dan senyawa bioaktif bernilai tambah, sekaligus meningkatkan efisiensi produksi DHA dan mendukung penerapan konsep ekonomi sirkular yang berkelanjutan.
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The global deficit of docosahexaenoic acid (DHA), estimated to exceed one million tons per year, is a significant issue in food security due to declining marine fish stocks and the unsustainable environmental impacts of conventional fish oil use. Thraustochytrids, as heterotrophic marine protists, have the ability to accumulate lipids up to approximately 50% of their dry biomass, making them a potential alternative source of DHA. However, the high cost of commercial carbon substrates such as glucose remains a major obstacle in industrial-scale production. This study examines the use of industrial waste agar from Gracilaria sp. as a more economical and environmentally friendly alternative carbon source, considering that approximately 65–75% of the initial biomass of Gracilaria sp. generally becomes solid and liquid waste that has the potential to cause environmental pollution. This solid waste is processed through a hydrolysis process to convert complex polysaccharides into simple sugars that can be utilized by Thraustochytrids as a nutrient source. The research results show that the utilization of industrial waste can support the growth of Thraustochytrids biomass and produce DHA-rich lipids and value-added bioactive compounds, while increasing the efficiency of DHA production and supporting the implementation of a sustainable circular economy concept.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Asam Dokosaheksaenoat, Thraustochytrids, Gracilaria sp., Limbah Industri Agar Docosahexaenoic Acid, Thraustochytrids, Gracilaria sp., Industrial Waste Agar.
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering
Divisions:	Faculty of Vocational > 24305-Industrial Chemical Engineering Technology
Depositing User:	Cavela Arnetta Khatrunada
Date Deposited:	01 Aug 2026 06:12
Last Modified:	01 Aug 2026 06:12
URI:	http://repository.its.ac.id/id/eprint/141136

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