Supriadiputra, Mohammad Farhan (2026) Ekstraksi Microbial Oil Aspergillus oryzae dengan Substrat Gula Tandan Kosong Kelapa Sawit Menggunakan Metode Microwave-Assisted Extraction sebagai Bahan Baku Biodiesel. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Keterbatasan bahan baku biodiesel berbasis pangan mendorong pemanfaatan microbial oil sebagai sumber lipid alternatif yang terbarukan. Penelitian ini mengevaluasi Microwave-Assisted Extraction (MAE) untuk mengekstraksi microbial oil dari biomassa Aspergillus oryzae yang dikultivasi menggunakan gula hasil konversi tandan kosong kelapa sawit. Pengaruh daya microwave, waktu ekstraksi, dan suhu ekstraksi terhadap lipid content dan lipid yield dievaluasi menggunakan rancangan Taguchi L9 dengan kriteria larger-the-better. Kondisi eksperimen terbaik diperoleh pada daya 450 W, waktu ekstraksi 30 menit, dan suhu 45°C, dengan lipid content sebesar 45,9% dan lipid yield sebesar 0,022 g/g. Berdasarkan nilai delta rasio signal to noise (S/N), daya microwave merupakan faktor yang paling berpengaruh terhadap kedua respons. Microbial oil yang diperoleh mengandung saturated fatty acid (SFA) 43,757%, monounsaturated fatty acid (MUFA) 34,991%, dan polyunsaturated fatty acid (PUFA) 21,252%. Berdasarkan komposisi asam lemak tersebut, biodiesel diperkirakan memiliki angka setana 65,76, viskositas kinematik 4,52 mm²/s, titik nyala 120,84°C, dan cold filter plugging point sebesar −1,6°C. Angka setana dan viskositas memenuhi acuan standar American Society for Testing and Materials (ASTM) D6751 dan European Norm (EN) 14214, sedangkan titik nyala memenuhi EN 14214. Nilai cold filter plugging point juga berada di bawah batas acuan maksimum (5°C). Hasil penelitian menunjukkan bahwa MAE berpotensi dikembangkan sebagai metode ekstraksi microbial oil dari A. oryzae untuk bahan baku biodiesel terbarukan berbasis TKKS.
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The limited availability of food-based biodiesel feedstocks has encouraged the utilization of microbial oil as a renewable alternative lipid source. This study evaluated Microwave-Assisted Extraction (MAE) for extracting microbial oil from Aspergillus oryzae biomass cultivated using sugars derived from oil palm empty fruit bunches (OPEFB). The effects of microwave power, extraction time, and extraction temperature on lipid content and lipid yield were investigated using a Taguchi L9 design with the larger-the-better criterion. The best conditions were 450 W microwave power, 30 min extraction time, and 45°C extraction temperature, yielding a lipid content of 45,9% and a lipid yield of 0,022 g/g. Based on the delta signal-to-noise (S/N) ratio, microwave power was the most influential factor affecting both responses. The extracted microbial oil contained 43,757% saturated fatty acids (SFA), 34,991% monounsaturated fatty acids (MUFA), and 21,252% polyunsaturated fatty acids (PUFA). Based on this fatty acid composition, the predicted biodiesel exhibited a cetane number of 65,76, kinematic viscosity of 4,52 mm²/s, flash point of 120,84°C, and a cold filter plugging point of −1,6°C. The cetane number and kinematic viscosity satisfied American Society for Testing and Materials (ASTM) D6751 and European Norm (EN) 14214, while the flash point met EN 14214. The cold filter plugging point was also below the maximum reference limit of 5°C. These findings demonstrate the potential of MAE for microbial oil extraction from A. oryzae for renewable biodiesel production.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Biodiesel, microbial oil, microwave-assisted extraction, tandan kosong kelapa sawit, Biodiesel, microbial oil, microwave-assisted extraction, oil palm empty fruit bunches |
| Subjects: | Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD63 Extraction Q Science > QR Microbiology |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Mohammad Farhan Supriadiputra |
| Date Deposited: | 01 Aug 2026 06:57 |
| Last Modified: | 01 Aug 2026 06:57 |
| URI: | http://repository.its.ac.id/id/eprint/141386 |
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