Pramesti, Najma Kamila (2026) Fabrikasi Dan Karakterisasi Surface Coating Pva-asam Borat Dengan Variasi Parameter Spin Coating Untuk Aplikasi Mikrofluidik Biomedis. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Adhesi protein darah yang tidak terkendali pada dinding saluran mikro menjadi hambatan utama pengembangan perangkat mikrofluida biomedis karena berisiko memicu trombosis dan mendistorsi akurasi deteksi. Penelitian ini bertujuan memfabrikasi dan mengarakterisasi pelapis permukaan (surface coating) berbasis poli(vinil alkohol)-asam borat (PVA-BA) pada substrat kaca menggunakan metode spin coating dengan variasi parameter deposisi demi mengevaluasi potensinya sebagai material hidrofilik dan biokompatibel. Larutan PVA 5% (b/v) dengan asam borat 1% (b/v) dideposisikan melalui sembilan variasi kombinasi kecepatan putar (500, 1000, 1500 rpm) dan durasi pemutaran (5, 10, 15 detik). Evaluasi lapisan mencakup analisis ATR-FTIR, pengukuran sudut kontak, uji penyerapan (swelling test), Atomic Force Microscopy (AFM), serta uji sitotoksisitas MTT. Hasil spektroskopi ATR-FTIR mengonfirmasi terjadinya ikatan silang (crosslinking) yang ditandai oleh pergeseran puncak O-H dari 3316,256 cm⁻¹ ke 3256,959 cm⁻¹, serta munculnya puncak B-O pada 1397,452 cm⁻¹ dan B-O-C pada 1155,222 cm⁻¹. Seluruh sampel menunjukkan sifat hidrofilik dengan rentang sudut kontak sebesar 32,38° - 40,54°, karakteristik hidrofilisitas ini lebih didominasi oleh densitas gugus -OH pada rantai PVA. Kondisi optimum dicapai pada parameter 1000 rpm selama 10 detik karena menghasilkan standar deviasi terkecil (1,593°). Lapisan pada parameter optimal ini menunjukkan stabilitas struktural yang baik dalam media berair dengan rasio pembengkakan (swelling ratio) sebesar 0,501% (1 jam) dan 0,835% (24 jam). Analisis AFM memperlihatkan variasi kekasaran spasial (Sa = 8,23 - 110 nm) dengan morfologi permukaan yang didominasi oleh struktur lembah, tetapi konsistensi keterbasahan makroskopisnya tetap stabil. Terakhir, uji MTT pada sel Vero membuktikan bahwa lapisan PVA-asam borat bersifat nonsitotoksik dengan viabilitas sel mencapai 103,85% ± 1,30%, yang telah melampaui standar biokompatibilitas ISO 10993-5 (≥70%). Dengan demikian, lapisan PVA-asam borat yang disintesis melalui spin coating ini terbukti hidrofilik, stabil dalam air, dan biokompatibel, sehingga sangat potensial diaplikasikan sebagai material pelapis pada mikrofluida biomedis.
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Uncontrolled adhesion of blood proteins to the walls of microchannels is a major obstacle to the development of biomedical microfluidic devices because it risks triggering thrombosis and compromising detection accuracy. This study aims to fabricate and characterize a poly(vinyl alcohol)-boric acid (PVA-BA)-based surface coating on glass substrates using the spin-coating method with varying deposition parameters to evaluate its potential as a hydrophilic and biocompatible material. A 5% (w/v) PVA solution with 1% (w/v) boric acid was deposited using nine different combinations of spin speeds (500, 1000, 1500 rpm) and spin durations (5, 10, 15 seconds). Evaluation of the coatings included ATR-FTIR analysis, contact angle measurements, a swelling test, Atomic Force Microscopy (AFM), and an MTT cytotoxicity assay. The ATR-FTIR spectroscopy results confirmed the occurrence of crosslinking, as indicated by a shift in the O-H peak from 3316.256 cm⁻¹ to 3256.959 cm⁻¹, as well as the appearance of B-O peaks at 1397.452 cm⁻¹ and B-O-C peaks at 1155.222 cm⁻¹. All samples exhibited hydrophilic properties with contact angles ranging from 32.38° to 40.54°; this hydrophilicity was primarily determined by the density of -OH groups on the PVA chain. Optimal conditions were achieved at 1000 rpm for 10 seconds, as this produced the smallest standard deviation (1.593°). The film formed under these optimal conditions exhibited good structural stability in an aqueous medium, with swelling ratios of 0.501% (1 hour) and 0.835% (24 hours). AFM analysis revealed variations in spatial roughness (Sa = 8.23–110 nm) with a surface morphology dominated by valley-like structures, but its macroscopic wettability remained stable. Finally, an MTT assay on Vero cells demonstrated that the PVA-boric acid coating is non-cytotoxic, with cell viability reaching 103.85 ± 1.30 %, which exceeds the ISO 10993-5 biocompatibility standard (≥70%). Thus, the PVA-boric acid coating synthesized via spin coating has been proven to be hydrophilic, stable in water, and biocompatible, making it highly suitable for use as a coating material in biomedical microfluidics.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biokompatibilitas, Hidrofilisitas, Mikrofluida, PVA-Asam Borat, Spin coating. Biocompatibility, Hydrophilicity, Microfluidics, PVA-Boric Acid, Spin coating. |
| Subjects: | Q Science > QC Physics > QC162 Adsorption and absorption |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45201-(S1) Undergraduate Thesis |
| Depositing User: | Najma Kamila Pramesti |
| Date Deposited: | 01 Aug 2026 02:23 |
| Last Modified: | 01 Aug 2026 02:23 |
| URI: | http://repository.its.ac.id/id/eprint/141534 |
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