Husna, Etika Ziadana Al (2026) Potensi antikanker nanopartikel perak hasil biosintesis menggunakan tanaman Pogostemon cablin Benth. pada lini sel kanker serviks (HeLa). Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kanker serviks menempati posisi keempat sebagai penyebab kematian akibat kanker pada perempuan yang sebagian besar disebabkan infeksi HPV onkogenik. Pengobatan konvensional seperti kemoterapi sering menimbulkan efek samping berat, sehingga dibutuhkan alternatif yang lebih aman. Nanopartikel perak (AgNPs) hasil biosintesis menggunakan ekstrak tanaman dinilai berpotensi sebagai agen antikanker yang ramah lingkungan. Daun nilam (Pogostemon cablin Benth.) mengandung senyawa bioaktif yang dapat berfungsi sebagai agen pereduksi dalam biosintesis AgNPs sekaligus mendukung aktivitas biologisnya. Namun penerapannya pada sel kanker serviks (HeLa) belum banyak diteliti. Pada penelitian ini, AgNPs dari ekstrak daun nilam dikarakterisasi menggunakan Spectrophotometer UV-Vis dan Transmission Electron Microscopy (TEM), kemudian dievaluasi aktivitas antikankernya pada sel HeLa melalui uji sitotoksisitas, uji apoptosis, analisis siklus sel, serta uji ekspresi gen p53 dan Cyclin D. Data dianalisis secara deskriptif kualitatif dengan penyajian tabel, grafik, dan visualisasi gambar. Hasil menunjukkan AgNPs memiliki potensi sebagai agen antikanker terhadap sel kanker serviks (HeLa) dengan aktivitas sitotoksik yang sangat aktif (IC₅₀ = 14,97 µg/mL), induksi apoptosis yang lebih tinggi (54,4%) dibandingkan cisplatin (22,25%), mengarahkan sel menuju arrest pada fase G0/G1 serta memodulasi ekspresi gen Cyclin D dan p53. Dengan demikian, AgNPs dari ekstrak daun nilam menunjukkan potensi sebagai agen antikanker alternatif melalui mekanisme multitarget, meliputi induksi sitotoksisitas, aktivasi apoptosis, arrest siklus sel, serta modulasi ekspresi gen regulator siklus sel dan respons kerusakan DNA pada sel kanker serviks (HeLa).
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Cervical cancer ranks fourth as a cause of cancer death in women, mostly caused by oncogenic HPV infection. Conventional treatments like chemotherapy often cause severe side effects, so safer alternatives are needed. Silver nanoparticles (AgNPs) biosynthesized using plant extracts are considered potentially eco-friendly anticancer agents. Patchouli leaves (Pogostemon cablin Benth.) contain bioactive compounds that can act as reducing agents in AgNP biosynthesis while supporting their biological activity. However, their application on cervical cancer cells (HeLa) has not been widely studied. In this study, AgNPs from patchouli leaf extract were characterized using UV-Vis Spectrophotometer and Transmission Electron Microscopy (TEM), and then their anticancer activity on HeLa cells was evaluated through cytotoxicity tests, apoptosis tests, cell cycle analysis, and p53 and Cyclin D gene expression assays. The data was analyzed descriptively and qualitatively using tables, graphs, and image visualizations. The results show that AgNPs have potential as an anticancer agent against cervical cancer cells (HeLa) with very active cytotoxic activity (IC₅₀ = 14.97 µg/mL), higher apoptosis induction (54.4%) compared to cisplatin (22.25%), leading cells toward G0/G1 phase arrest, and modulating the expression of Cyclin D and p53 genes. Thus, AgNPs from patchouli leaf extract show potential as an alternative anticancer agent through a multitarget mechanism, including inducing cytotoxicity, activating apoptosis, cell cycle arrest, and modulating the expression of cell cycle regulatory genes and DNA damage response in cervical cancer cells (HeLa).
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | AgNPs, antikanker, HeLa, kanker serviks, Pogostemon cablin Benth., AgNPs, anticancer, cervical cancer, HeLa, Pogostemon cablin Benth. |
| Subjects: | Q Science > QH Biology Q Science > QH Biology > QH301 Biology Q Science > QK Botany > QK14.5 Botanical literature (Including works on herbals) Q Science > QP Physiology Q Science > QP Physiology > QP624 Molecular biology. R Medicine > R Medicine (General) > R857.M3 Biomedical materials. Biomedical materials--Testing. |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis |
| Depositing User: | Etika Ziadana Al Husna |
| Date Deposited: | 04 Aug 2026 01:52 |
| Last Modified: | 04 Aug 2026 01:52 |
| URI: | http://repository.its.ac.id/id/eprint/142664 |
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