Fabrikasi dan Evaluasi Kinerja Sensor Elektrokimia SPCE Termodifikasi Fe3O4/Nafion untuk Deteksi Hidrogen Peroksida (H2O2)

Khusna, Fadhilah Untsatul Khusna (2026) Fabrikasi dan Evaluasi Kinerja Sensor Elektrokimia SPCE Termodifikasi Fe3O4/Nafion untuk Deteksi Hidrogen Peroksida (H2O2). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Hidrogen peroksida (H2O2) merupakan senyawa yang banyak digunakan dalam industri pangan sebagai desinfektan dan agen sterilisasi. Keberadaan residu H2O2 dalam produk pangan perlu dikontrol karena dapat menimbulkan dampak negatif bagi kesehatan apabila melebihi batas yang diperbolehkan. Penelitian ini bertujuan untuk mensintesis nanopartikel Fe3O4, memfabrikasi sensor elektrokimia berbasis Screen Printed Carbon Electrode (SPCE) termodifikasi Fe3O4/Nafion, serta mengevaluasi kinerjanya untuk deteksi H₂O₂. Nanopartikel Fe3O4 disintesis menggunakan metode elektrolisis dan dikarakterisasi menggunakan FE-SEM/EDX serta XRD. Hasil karakterisasi menunjukkan bahwa nanopartikel Fe3O4 memiliki morfologi relatif sferis dengan ukuran rata-rata 94,96 nm dan struktur kristal magnetit. Sensor SPCE/Nafion/Fe3O4 kemudian dievaluasi menggunakan metode Cyclic Voltammetry (CV) untuk menentukan kondisi optimum dan parameter analitik sensor. Hasil penelitian menunjukkan bahwa kondisi optimum diperoleh pada larutan PBS pH 7 dengan laju pindai 50 mV/s. Analisis pengaruh laju pindai menunjukkan bahwa mekanisme interaksi H2O2 dengan permukaan SPCE/Nafion/Fe3O4 dikontrol oleh proses difusi sehingga reaksi oksidasi H2O2 berlangsung secara diffusion-controlled. Sensor menunjukkan rentang linearitas terbaik pada konsentrasi H2O2 0,25–1 µM dengan nilai koefisien determinasi (R²) sebesar 0,99246, limit of detection (LOD) sebesar 0,842 µM, dan sensitivitas sebesar 318,598 µA/µM.cm². Uji selektivitas menunjukkan bahwa H2O2 menghasilkan puncak oksidasi yang jelas, sedangkan glukosa, asam urat, asam sitrat, asam askorbat, dan NaCl pada konsentrasi yang sama tidak menghasilkan respon yang signifikan. Selain itu, sensor memiliki repeatability dan reproducibility yang baik dengan nilai %RSD masing-masing sebesar 2,87 dan 1,91%. Hasil penelitian menunjukkan bahwa sensor SPCE/Nafion/Fe3O4 memiliki sensitivitas, linearitas, selektivitas, dan keterulangan yang baik sehingga berpotensi dikembangkan sebagai metode deteksi H2O2 yang sederhana, praktis, dan andal untuk aplikasi keamanan pangan.
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Hydrogen peroxide (H2O2) is a compound widely used in the food industry as a disinfectant and sterilizing agent. The presence of residual H2O2 in food products must be monitored because excessive levels may pose adverse health effects. This study aimed to synthesize Fe3O4 nanoparticles, fabricate a Fe3O4/Nafion-modified Screen Printed Carbon Electrode (SPCE)-based electrochemical sensor, and evaluate its performance for H2O2 detection. Fe3O4 nanoparticles were synthesized using an electrolysis method and characterized by FE-SEM/EDX and XRD analyses. The characterization results revealed that the Fe3O4 nanoparticles exhibited a relatively spherical morphology with an average particle size of 94.96 nm and a magnetite crystal structure. The fabricated SPCE/Nafion/Fe3O4 sensor was then evaluated using Cyclic Voltammetry (CV) to determine the optimum operating conditions and analytical performance parameters. The results showed that the optimum conditions were obtained in phosphate-buffered saline (PBS) at pH 7 with a scan rate of 50 mV/s. Scan rate analysis indicated that the interaction mechanism between H2O2 and the SPCE/Nafion/Fe3O4 surface was governed by a diffusion-controlled process, resulting in the oxidation reaction of H2O2 being diffusion-controlled. The sensor exhibited the best linear range at H2O2 concentrations of 0.25–1 µM, with a coefficient of determination (R²) of 0.99246, a limit of detection (LOD) of 0.842 µM, and a sensitivity of 318.598 µA/µM·cm². Selectivity tests demonstrated that H2O2 produced a distinct oxidation peak, whereas glucose, uric acid, citric acid, ascorbic acid, and NaCl at the same concentration did not generate significant responses. Furthermore, the sensor showed good repeatability and reproducibility, with %RSD values of 2.87 and 1.91%, respectively. These findings indicate that the SPCE/Nafion/Fe3O4 sensor possesses good sensitivity, linearity, selectivity, and repeatability, making it a promising, simple, practical, and reliable method for H2O2 detection in food safety applications.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Hidrogen Peroksida; Sensor Elektrokimia; SPCE; Fe3O4; Nafion; Hydrogen Peroxide; Electrochemical Sensor; SPCE; Fe3O4; Nafion.
Subjects:	Q Science > QD Chemistry > QD1 Oxidation-reduction reaction. Q Science > QD Chemistry > QD115 Electrochemical analysis Q Science > QD Chemistry > QD553 Electrochemistry. Electrolysis
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Fadhilah Untsatul Khusna
Date Deposited:	04 Aug 2026 06:48
Last Modified:	04 Aug 2026 06:48
URI:	http://repository.its.ac.id/id/eprint/143423

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