Astuti, Rani Widya (2026) Biodegradasi Pewarna Metilen Biru oleh Kultur Campuran Jamur Filamen Aspergillus oryzae dan Bakteri Pseudomonas aeruginosa yang Terimobilisasi pada Matriks SA/PVA/Bentonit. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Pencemaran air akibat limbah pewarna sintetis seperti metilen biru (MB) menjadi salah satu masalah lingkungan yang serius karena sifatnya yang toksik, persisten, dan sulit terurai secara alami. Pendekatan bioremediasi menggunakan mikroorganisme menawarkan solusi ramah lingkungan dan efisien untuk mengatasi permasalahan ini. Penelitian ini bertujuan untuk mengevaluasi kemampuan kultur campuran jamur filamen Aspergillus oryzae dan bakteri Pseudomonas aeruginosa dalam mendegradasi MB yang diimobilisasi pada matriks sodium alginat/polivinil alkohol/bentonit (SA/PVA/Bentonit). Hasil screening biodekolorisasi menunjukkan bahwa kultur campuran terimobilisasi memiliki performa terbaik sebesar 99,33% dibandingkan dengan sisitem sel bebas kultur campuran (82,56%) maupun matriks SA/PVA/Bentonit sebagai kontrol (98,35%). Adsorpsi pada matriks mengikuti model kinetika orde dua semu dan model isoterm Langmuir yang menunjukkan pembentukan lapisan tunggal pada permukaan beads yang homogen. Hasil optimasi RSM menunjukkan persentase dekolorisasi MB tertinggi mencapai 98,36% dengan kondisi optimal pada pH 6,68, suhu 37,41 oC, dan waktu inkubasi 385,75 menit. Proses biodegradasi dibuktikan melalui analisis instrumen LC-TOF/MS dengan munculnya senyawa-senyawa metabolit intermediet berukuran lebih kecil, yaitu Azure A, Azure B, Azure C, (Z)-(6-iminocyclohexa-2,4-dien-1-ylidene)(phenyl)sulfonium, dan 5-(dimethylamino)-2-nitrobenzenesulfonic acid, serta hasil biotransformasi yaitu 3,7-bis(dimethylamino)-4H-phenothiazine 5-oxide. Temuan ini akan dapat berkontribusi pada pengembangan teknologi bioremediasi yang berkelanjutan dan aplikatif bagi industri tekstil di Indonesia.
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Water pollution due to synthetic dye waste such as methylene blue (MB) is a serious environmental problem due to its toxic, persistent, and difficult to decompose naturally. Bioremediation approaches using microorganisms offer an environmentally friendly and efficient solution to overcome this problem. This study aimed to evaluate the ability of a mixed culture of filamentous fungus Aspergillus oryzae and bacterium Pseudomonas aeruginosa to degrade MB immobilized on a sodium alginate/polyvinyl alcohol/bentonite (SA/PVA/Bentonite) matrix. The results of biodecolorization screening showed that the immobilized mixed culture had the best performance of 99.33% compared to the free cell culture system (82.56%) and SA/PVA/Bentonite matrix as the control (98.35%). Adsorption on the matrix followed a pseudo-second-order kinetic model and a Langmuir isotherm model that showed the formation of a homogeneous monolayer on the surface of the beads. The results of RSM optimization showed the highest MB decolorization percentage reaching 98.36% with optimal conditions at pH 6.68,temperature 37.41 oC, and incubation time 385.75 minutes. The biodegradation process was proven through LC-TOF/MS instrument analysis with the emergence of smaller intermediate metabolite compounds Azure A, Azure B, Azure C, (Z)-(6-iminocyclohexa-2,4-dien-1-ylidene)(phenyl)sulfonium, and 5-(dimethylamino)-2-nitrobenzenesulfonic acid, as well as the biotransformation product, namely 3,7-bis(dimethylamino)-4H-phenothiazine 5-oxide. These findings will be able to contribute to the development of sustainable and applicable bioremediation technology for the textile industry in Indonesia.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Bioremediasi, Pseudomonas aeruginosa, Aspergillus oryzae, Metilen biru, Imobilisasi, SA/PVA/bentonit. Bioremediation, Pseudomonas aeruginosa, Aspergillus oryzae, Methylene blue, Immobilization, SA/PVA/bentonite. |
| Subjects: | Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology T Technology > TD Environmental technology. Sanitary engineering > TD192.5 Bioremediation |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis |
| Depositing User: | Rani Widya Astuti |
| Date Deposited: | 05 Aug 2026 02:01 |
| Last Modified: | 05 Aug 2026 02:01 |
| URI: | http://repository.its.ac.id/id/eprint/143822 |
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