Deteksi Dopamin Pada Kondisi Asam Menggunakan Screen Printed Carbon Electrode

Illiya, Wihda (2022) Deteksi Dopamin Pada Kondisi Asam Menggunakan Screen Printed Carbon Electrode. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sensor elektrokimia untuk deteksi dopamin masih memiliki kekurangan
yakni selektivitas yang rendah. Pada penelitian ini telah dilakukan deteksi dopamin
pada kondisi asam menggunakan sensor elektrokimia berbasis Screen Printed
Carbon Electrode (SPCE). Pengukuran dilakukan dengan metode voltametri siklik
pada potensial -0,750 V sampai 1,000 V. Larutan sampel dilarutkan dalam larutan
bufer fosfat 0,1 M pH 5. Limit deteksi SPCE untuk dopamin pada arus puncak
anodik dan arus puncak katodik berturut-turut sebesar 1,669 μM dan 0,384 μM.
Sensitivitas SPCE diperoleh sebesar 0,013 μA mm-2 μM-1 pada arus puncak anodik
dan 0,010 μA mm-2 μM-1 pada arus puncak katodik. Uji selektivitas dilakukan
dengan menguji respon SPCE terhadap larutan asam urat, asam askorbat, glukosa
dan urea. Selektivitas sensor terbaik diperoleh pada pengujian dengan bufer fosfat
0,1 M pH 5 karena hanya dopamin yang memunculkan arus puncak oksidasi dan
reduksi tanpa senyawa pengganggu lain. Deteksi dopamin menggunakan SPCE
dikontrol oleh proses difusi di permukaan elektroda.

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Electrochemical sensor for detection of dopamine still have drawbacks,
namely low selectivity. In this study, the detection of dopamine in acidic condition
was carried out using an electrochemical sensor based on Screen Printed Carbon
Electrode (SPCE). Measurements were carried out using the cyclic voltammetry
method at a potential of -0.750 V to 1.000 V. The sample solution was dissolved in
0.1 M phosphate buffer pH 5. The detection limit of SPCE for dopamine at anodic
and cathodic peak current were 1.669 μM and 0.384 μM, respectively. The
sensitivity of SPCE was obtained at 0.013 μA mm-2 μM-1 at anodic peak current and
0.010 μA mm-2 μM-1 at cathodic peak current. The selectivity test was carried out
by testing the SPCE response to uric acid, ascorbic acid, glucose and urea solutions.
The best sensor selectivity was obtained in testing with 0.1 M phosphate buffer pH
5 because only dopamine generated peak currents of oxidation and reduction
without other interfering compounds. Detection of dopamine using a SPCE sensor
is controlled by a diffusion process on the surface of the electrode.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Biosensor, Dopamin, Elektrokimia, SPCE, Voltametri.
Subjects:	Q Science > QD Chemistry > QD115 Electrochemical analysis
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	magang .
Date Deposited:	31 Aug 2026 02:21
Last Modified:	31 Aug 2026 02:21
URI:	http://repository.its.ac.id/id/eprint/144423

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