Sensor Elektrokimia Berbasis Screen Printed Carbon Electrode (Spce) Untuk Deteksi Dopamin Pada Kondisi Netral

Permatasari, Lia Marisca (2022) Sensor Elektrokimia Berbasis Screen Printed Carbon Electrode (Spce) Untuk Deteksi Dopamin Pada Kondisi Netral. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sensor elektrokimia untuk mendeteksi dopamin telah dipelajari menggunakan
Screen Printed Carbon Electrode (SPCE). Kinerja SPCE dikarakterisasi
menggunakan voltametri siklik (CV) pada pH 7 dalam larutan buffer fosfat 0,1 M.
Pengukuran dilakukan pada kisaran potensial -0,750 hingga 1,000 V. Pengaruh
konsentrasi dopamin dan laju sapuan dipelajari. Hasil penelitian menunjukkan
bahwa dopamin dapat dideteksi secara spesifik dengan melihat sinyal puncak
anodik dan katodiknya masing-masing pada potensial 0,186 V dan -0,006 V. Sinyal
dopamin dapat terdeteksi tanpa adanya gangguan sinyal dari senyawa interferensi
asam askorbat, asam urat, glukosa dan urea. Kurva kalibrasi yang merupakan plot
antara variasi konsentrasi dopamin terhadap arus puncak anodik dan katodik
mengikuti persamaan linier, Ipa = 3,484+0,413x, dengan R2 = 0,992 dan Ipc = -2,741
0,267x, dengan R2 = 0,985. Limit deteksi (LOD) dan sensitivitas SPCE untuk
deteksi Dopamin masing-masing adalah 0,0319 μM; 0,1986 μA mm-2 μM-1 untuk
puncak anodik dan 0,2512 μM; 0,1532 μA mm-2 μM-1 untuk puncak katodik.
Pengaruh laju sapuan menunjukkan interaksi antara SPCE dengan larutan uji
dopamin dikendalikan oleh proses difusi. Uji repeatability SPCE menunjukkan
peningkatan nilai arus puncak yang mengakibatkan kurang presisinya pengukuran
berulang.

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Electrochemical sensors for detecting dopamine have been studied using a Screen
Printed Carbon Electrode (SPCE). SPCE performance was characterized using
Cyclic Voltammetry (CV) at pH 7 in 0.1 M phosphate buffer solution.
Measurements were made in the potential range of -0.750 to 1.000 V. Effect of
dopamine concentration and scane rate. The results showed that dopamine can be
detected specifically by looking at the anodic and cathodic peak signals at a
potential of 0.186 V and -0.006 V, respectively. Dopamine signals can detect
without any signal interference from the interference compounds of ascorbic acid,
uric acid, glucose and urea. The calibration curve which is a plot between the
variation of dopamine concentration on anodic and cathodic peak currents follows
a linear equation, Ipa = 3.484+0.413x, with R2 = 0.992 and Ipc = -2.741-0.267x, with
R2 = 0.985. The limit of detection (LOD) and SPCE sensitivity for detecting
dopamine were 0.0319 µM, respectively; 0.1866 µA mm-2 µM-1 for the anodic peak
and 0.2512 µM; 0.1532 µA mm-2 µM-1 for the cathodic peak. The effect of scan
rate shows the interaction between SPCE and the test solution of dopamine which
is controlled by the diffusion process. The SPCE repeatability test showed an
increase in the peak current value which results in a lack of precision in repeated
measurements.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Electrochemical Sensor, Screen Printed Carbon Electrode, Dopamine, Voltammetry, Neutral Condition.
Subjects:	Q Science > QD Chemistry > QD553 Electrochemistry. Electrolysis
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	magang .
Date Deposited:	31 Aug 2026 06:38
Last Modified:	31 Aug 2026 06:38
URI:	http://repository.its.ac.id/id/eprint/144427

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