Respon pertumbuhan dan fisiologi akar wangi (Chrysopogon zizanioides) terhadap cekaman petroleum hydrocarbon pada tanah pascatambang minyak bumi kabupaten bojonegoro

Firdaus, Abiyyu Armijn Firman (2022) Respon pertumbuhan dan fisiologi akar wangi (Chrysopogon zizanioides) terhadap cekaman petroleum hydrocarbon pada tanah pascatambang minyak bumi kabupaten bojonegoro. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Akar wangi (Chrysopogon zizanioides) merupakan salah satu tanaman yang dapat digunakan untuk memperbaiki kualitas tanah, utamanya dalam degradasi polutan. Lahan pascatambang sebagai lingkungan yang cukup ekstrem identik dengan adanya kerusakan tanah secara fisik dan kimiawi, sehingga menjadi salah satu faktor cekaman bagi tanaman, tak terkecuali pada tanah pascatambang minyak bumi di Kabupaten Bojonegoro. Penelitian ini bertujuan untuk mengetahui respons pertumbuhan dan fisiologi akar wangi terhadap cekaman Petroleum Hydrocarbon (PHC) dengan perlakuan perbedaan konsentrasi. Penelitian ini melalui uji pendahuluan berupa analisis pH dan kadar PHC awal pada tanah dengan metode spektrofotometri serta aklimatisasi tanaman. Pemberian perlakuan dilakukan selama 45 hari dengan variasi konsentrasi tanah tercemar 0%, 25%, 50%, 75%, dan 100% dari berat total tanah. Parameter pertumbuhan meliputi panjang akar, tinggi tajuk, jumlah daun total, berat basah dan kering tajuk serta akar; serta parameter fisiologi meliputi persentase kadar air pada tanaman serta kadar klorofil total dengan metode spektrofotometri. Efisiensi penyisihan kadar PHC dan pH tanah akhir diukur sebagai data pendukung setelah hari perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dan uji statistik ANOVA One Way. Hasil penelitian didapatkan kadar PHC awal sebesar 80441,02 mg/kg dengan pH tanah pada kisaran 4,0–6,4. Pemberian cekaman PHC terhadap tanaman akar wangi dengan perlakuan perbedaan konsentrasi tanah tercemar berpengaruh terhadap panjang akar, tinggi tajuk, berat basah dan kering tajuk serta akar, persentase kadar air pada tanaman, dan kadar klorofil total. Konsentrasi PHC tertinggi memberikan respons positif terhadap rata-rata panjang akar sebesar 21,00 ± 2,00 cm, tinggi tajuk sebesar 120,00 ± 3,00 cm, berat basah akar sebesar 5,99 ± 0,31 g, dan berat kering akar sebesar 0,90 ± 0,05 g; sedangkan memberikan respons negatif terhadap rata-rata berat basah tajuk sebesar 6,90 ± 0,43 g dan berat kering tajuk sebesar 1,38 ± 0,09 g, kadar air pada tajuk berkurang hingga 65% dan pada akar 80%, serta kadar klorofil total sebesar 33,73 ± 5,49 mg/L. Efisiensi penyisihan PHC semakin optimal seiring dengan konsentrasi tanah tercemar yang semakin sedikit, yakni 33,37% pada konsentrasi tanah tercemar PHC 25%, dan pH tanah akhir pada kisaran 4,2–6,6.
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Vetiver (Chrysopogon zizanioides) is one of the plants that can be used to improve soil quality, especially in pollutant degradation. Post-mining land, as a relatively extreme environment, is characterized by physical and chemical soil degradation, making it one of the stress factors for plants, including those growing in post-oil-mining soil in Bojonegoro Regency. This study aims to determine the growth and physiological responses of vetiver to Petroleum Hydrocarbon (PHC) stress under different concentration treatments. This research was conducted through a preliminary test involving the analysis of pH and initial PHC levels in the soil using spectrophotometric methods, as well as plant acclimatization. The treatment was carried out for 45 days with variations in contaminated soil concentrations of 0%, 25%, 50%, 75%, and 100% of the total soil weight. Growth parameters included root length, crown height, total number of leaves, and wet and dry weights of the crown and roots; physiological parameters included the percentage of water content in the plants and total chlorophyll content measured using spectrophotometric methods. PHC removal efficiency and final soil pH were measured as supporting data after the treatment period. This study used a Completely Randomized Design (CRD) and a One-Way ANOVA statistical test. The results showed that the initial PHC level was 80,441.02 mg/kg, with soil pH ranging from 4.0–6.4. The application of PHC stress to vetiver plants under different contaminated soil concentrations affected root length, crown height, wet and dry weights of the crown and roots, percentage of water content in the plants, and total chlorophyll content. The highest PHC concentration produced a positive response in terms of the average root length of 21.00 ± 2.00 cm, crown height of 120.00 ± 3.00 cm, root wet weight of 5.99 ± 0.31 g, and root dry weight of 0.90 ± 0.05 g; while producing a negative response in terms of the average crown wet weight of 6.90 ± 0.43 g and crown dry weight of 1.38 ± 0.09 g, with water content decreasing to 65% in the crown and 80% in the roots, and total chlorophyll content of 33.73 ± 5.49 mg/L. The efficiency of PHC removal increased as the concentration of contaminated soil decreased, reaching 33.37% at a PHC-contaminated soil concentration of 25%, with the final soil pH ranging from 4.2–6.6.

Item Type:	Thesis (Other)
Additional Information:	571.849 29 Fir r-1
Uncontrolled Keywords:	Akar wangi, Cekaman PHC, Kabupaten Bojonegoro, Respon pertumbuhan dan fisiologi tanaman, Tanah pascatambang minyak bumi. , Kabupaten Bojonegoro, PHC stress, Post-oil mining soil, Response of the growth and physiological, Vetiver.
Subjects:	Q Science > QK Botany > QK731 Growth (Plants)
Divisions:	Faculty of Natural Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	magang .
Date Deposited:	30 Sep 2026 06:11
Last Modified:	30 Sep 2026 06:11
URI:	http://repository.its.ac.id/id/eprint/145081

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