Isolasi Biflavonoid Dari Ekstrak Etil Asetat Kayu Batang Garcinia Celebica Linn

Najiyah, Ati (2008) Isolasi Biflavonoid Dari Ekstrak Etil Asetat Kayu Batang Garcinia Celebica Linn. Masters thesis, Institut Teknologi Sepuluh November.

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Abstract

Dua senyawa turunan biflavonoid yaitu 5,7,2',5",7",4'"-heksahidroksiflavanon-[3,8"]-flavon (1) dan 5,7,2',3",5",7",4"-heptahidroksi-[3,8"]-biflavanon (2), dapat diisolasi dari kayu batang Garcinia celebica Linn. Isolasi dilakukan dengan cara maserasi menggunakan pelarut etil asetat. Pemisahan komponen dilakukan dengan kromatografi cair vakum (KCV), kromatografi kolom gravitasi (KKG), kromatografi lapis tipis preparatif (KLTP) dan pemurnian dengan metode KLTP dan rekristalisasi menggunakan campuran pelarut kloroform-metanol. Penentuan struktur dengan memanfaatkan data spektroskopi UV, IR, 1H-NMR dan 13C-NMR. Pengujian aktivitas antibakteri dengan metode difusi agar Kirby-Bauer terhadap bakteri Salmonella typhi, menghasilkan petunjuk bahwa kedua senyawa tersebut relatif kurang aktif dibandingkan kloramfenikol.
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Two biflavonoid derivative compounds namely 5,7,2',5",7",4'"-hexahydroxyflavanone-[3,8"]-flavone (1) and 5,7,2',3",5",7",4"-heptahydroxy-[3,8"]-biflavanone (2), have been isolated from the stem wood of Garcinia celebica Linn. These compounds were isolated by maceration using ethyl acetate. Compounds were fractionated by vacuum liquid chromatography, column chromatography, preparative thin layer chromatography (preparative TLC) and then purified by preparative TLC and recrystallization using a mixture of chloroform and methanol solvent. The compounds' structure was elucidated by spectroscopy methods namely UV, IR, 1H-NMR and 13C-NMR. Antibacterial assay using Kirby-Bauer method against Salmonella typhi showed that both compounds have less activity than chloramphenicol.

Item Type:	Thesis (Masters)
Additional Information:	RTKi 583.624 Naj i
Uncontrolled Keywords:	Garcinia celebica Linn, biflavonoid, antibakteri, Garcinia celebica Linn, biflavonoid, antibacterial.
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis
Depositing User:	magang .
Date Deposited:	02 Oct 2026 07:59
Last Modified:	02 Oct 2026 07:59
URI:	http://repository.its.ac.id/id/eprint/145209

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