RESPON CEKAMAN GENANGAN PERIODIK PADA BEBERAPA VARIETAS Nicotiana tabacum

RAKHMAN, R. YUVITA (2016) RESPON CEKAMAN GENANGAN PERIODIK PADA BEBERAPA VARIETAS Nicotiana tabacum. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

[img]
Preview
Text
1512100018-Undergraduate_theses.pdf - Published Version

Download (2MB) | Preview

Abstract

Genangan dapat menyebabkan hasil produksi suatu tanaman menurun, utamanya pada tanaman terestrial seperti tembakau. Sehingga, dapat meningkatkan kerugian petani tembakau. Penelitian ini bertujuan untuk mengetahui respon Nicotiana tabacum yang dicekam genangan periodik ditinjau dari aspek anatomi, fisiologi, morfologi dan biokimia. Empat varietas tanaman tembakau (Jepon Pelakean, Jinten, Manilo dan Marakot) dicekam genangan periodik pada fase vegetatif dengan tahapan waterlogging selama lima hari dan dilanjutkan dengan partial submergence selama lima hari berikutnya. Data pengamatan dianalisis menggunakan ANNOVA One-Way dengan taraf kepercayaan 95% untuk menguji parameter morfologi dan fisiologi. Pada aspek morfologi, Jepon Pelakean memiliki respon lebih baik pada parameter tinggi tanaman, panjang akar, diameter batang, lebar daun dan jumlah akar adventif dibanding ketiga varietas uji lain. Rerata penutupan stomata tertinggi pada Manilo (95,2%), penurunan klorofil tertinggi terjadi pada Manilo sebesar (41,5%), dan berat kering tertinggi oleh Jepon Pelakean sebesar (1,17 mg). Hasil analisis SDS-PAGE pada Jepon Pelakean, Manilo dan Marakot menunjukkan bahwa terdapat peningkatan ekspresi protein dengan berat molekul 134,98 kDa secara konsisten di kondisi waterlogging. Sedangkan, Jinten menunjukkan ekspresi tersebut saat partial submergence. ============================================================ Flooding could decrease the yield of any terrestrial plant, including tobacco. Thus condition may lead to economic losses for the farmers. The aims of the research are to evaluate the responses of tobacco (Nicotiana tabacum) plant to periodic flooding stress based on anatomy, physiology, morphology and biochemistry aspects. Four varieties of tobacco (Jepon Pelakean, Jinten, Manilo and Marakot) were treated by flooding stress during vegetative phase; by means of waterlogging stress for five days continued by partial submergence for the next five days. Data were analyzed using One-Way ANNOVA at 95% confidence level to test the morphological and physiological aspects. In the morphology aspect, Jepon Pelakean have better responses on parameters of plant height, root length, stem diameter, leaf width and number of adventitious roots than the other three varieties. Furthermore, highest mean of stomatal closure occurred on Manilo (95.2%), highest chlorophyll decrement occurred in Manilo (41.5%), and highest dry weight occurred in Jepon Pelakean (1.17 mg). The results of SDS-PAGE analysis of Jepon Pelakean, Manilo and Marakot show increased expression of a protein with molecular weight of 134.98 kDa consistently in waterlogging treatment. Meanwhile, Jinten showed that expression in partial submergence.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 583.952 Rak r
Uncontrolled Keywords: partial submergence, profil protein, tembakau, waterlogging
Subjects: Q Science > QH Biology > QH301 Biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Mrs Anis Wulandari
Date Deposited: 11 Jan 2017 03:54
Last Modified: 27 Dec 2018 06:19
URI: http://repository.its.ac.id/id/eprint/1468

Actions (login required)

View Item View Item