Hidayatika, Uchik Nur (2015) Isolasi Senyawa Metabolit Sekunder Dan Uji Antioksidan Dari Ranting Garcinia Balica. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Senyawa 2,2,3,5,5-pentametil-1-pentanol (1) telah
berhasil diisolasi dari ekstrak metanol ranting Garcinia balica asal
Taman Nasional Baluran, Situbondo, Jawa Timur berbentuk pasta
oil tak berwarna dengan titik leleh 67oC - 68oC. Metode isolasi
menggunakan Kromatografi Cair Vacum (KCV) dielusi dengan
pelarut yang ditingkatkan kepolarannya dan Kromatografi Lapis
Tipis Preparatif (KLTP). Penentuan struktur menggunakan hasil
teknik NMR-1D dan NMR 2-D serta spektrofotometer IR.
Aktivitas antioksidan ditentukan menggunakan metode uji DPPH
(2,2-diphenyl-1-picrylhydrazyl). Senyawa 1 menunjukkan mL
tidak aktif terhadap pengujian DPPH sedangkan Fraksi C
menunjukkan penghambatan dengan nilai IC50 86,50 μg/m namun
aktivitas penghambatan masih dibawah trolox sebagai kontrol
positif
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2,2,3,5,5-pentametil-1-pentanol (1) was isolated from
crude methanol extract of the branch of Garcinia balica from
Baluran National Park, Situbondo, Jawa Timur as colourless pasta
oil with melting point 67oC - 68oC. Isolation method used vacuum
liquid chromathography eluted with increasing polarity solvent
and preparative thin later chromatography. Its structure was
elucidated using 1D and 2D NMR techniques along with IR
spectrophotometry. The antioxidant activity was determined using
DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Compound 1
menunjukkan showed negative antioxidant activity. Fraction of C
showed inhibition on DPPH with IC50 value 86,50 μg/m but
inhibition activity is lower than trolox as a positive control.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSKi 543.089 Hid i |
| Uncontrolled Keywords: | Garcinia balica, Clusiaceae, senyawa metabolit sekunder, aktivitas antioksidan, DPPH. |
| Subjects: | T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions. |
| Divisions: | Faculty of Mathematics and Science > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Mr. Tondo Indra Nyata |
| Date Deposited: | 10 Jul 2017 06:16 |
| Last Modified: | 24 Aug 2018 03:10 |
| URI: | http://repository.its.ac.id/id/eprint/41965 |
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