Fajariah, Hayuni Devina (2012) Pemanfaatan Serbuk Gergaji Menjadi Biobutanol Dengan Proses Hidrolisis Selulase Dan Fermentasi Bakteri Clostridium Acetobutylicum. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Biobutanol adalah jenis alkohol ikatan C-4 (C4H9OH)
yang terbuat dari biomassa. Penelitian ini dilakukan dengan
memanfaatkan limbah kayu yang dihasilkan dari proses
penggergajian kayu yang mengandung selulosa (55%),
hemiselulosa (14%), dan lignin (21%). Biobutanol diproduksi
dengan cara hidrolisis enzim selulase dan fermentasi bakteri
Clostridium acetobutylicum. Variabel pada penelitian ini adalah
penambahan enzim selulase pada proses hidrolisis (penambahan
enzim atau tanpa penambahan enzim), pH awal proses fermentasi
(5 atau 7) dan jumlah penambahan starter bakteri Clostridium
acetobutylicum (5 atau 10 ml) dengan variasi lama proses
fermentasi 2,4,6,8,10,12 hari. Parameter dalam penelitian ini
adalah analisa kadar selulosa, gula tereduksi, dan kadar butanol.
Berdasarkan hasil penetian, diketahui bahwa proses
hidrolisis dengan penambahan enzim selulase, kondisi awal
fermentasi pH 5 dan penambahan inokulum bakteri Clostridium
acetobutylicum sebanyak 10 ml dengan lama waktu fermentasi 12
hari merupakan kondisi yang paling efektif menghasilkan kadar
butanol tertinggi dari 50 gram limbah serbuk gergaji. Kadar
butanol tertinggi sebesar 1,88 % dari 1 μL sampel hasil
fermentasi yang diinjeksikan ke dalam kromatografi gas.
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Biobutanol is a type of alcohol-bond C-4 (C4H9OH) is
made from biomass. This research was carried out by utilizing
sawdust generated from the sawmill which contain cellulose
(55%), hemicellulose (14%), and lignin (21%). Biobutanol is
produced by hydrolysis of cellulase enzymes and fermenting
bacterium Clostridium acetobutylicum. Variables in this study is
addition of cellulase enzymes on the hydrolysis (with or without
addition of enzyme), the initial pH of fermentation processes (5 or
7) and the addition of starter bacteria Clostridium acetobutylicum
(5 or 10 ml) with a time variation process of fermentation 2,4, 6,
8, 10, 12 days. The parameters in this study is cellulose analysis,
sugar reduced, and levels of butanol.
Based on results of this research, known that hydrolysis
by addition of cellulase enzymes, initial conditions of
fermentation pH 5 and addition of bacterial inoculum of 10 ml of
Clostridium acetobutylicum with fermentation time of 12 days is
the most effective conditions to produce the highest levels of
butanol from 50 grams sawdust. The highest levels of butanol are
1,88 % from 1 μL of fermentation that injected into the gas
chromatograph.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSL 674.84 Faj p |
| Uncontrolled Keywords: | biobutanol, Clostridium acetobutylicum, selulase, serbuk gergaji, cellulase, sawdust. |
| Subjects: | T Technology > TD Environmental technology. Sanitary engineering |
| Divisions: | Faculty of Civil Engineering and Planning > Environment Engineering > 25201-(S1) Undergraduate Thesis |
| Depositing User: | Anis Wulandari |
| Date Deposited: | 24 Aug 2017 02:33 |
| Last Modified: | 24 Aug 2017 02:33 |
| URI: | http://repository.its.ac.id/id/eprint/48746 |
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