Pemanfaatan Serbuk Gergaji Menjadi Biobutanol Dengan Proses Hidrolisis Selulase Dan Fermentasi Bakteri Clostridium Acetobutylicum

Fajariah, Hayuni Devina (2012) Pemanfaatan Serbuk Gergaji Menjadi Biobutanol Dengan Proses Hidrolisis Selulase Dan Fermentasi Bakteri Clostridium Acetobutylicum. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Biobutanol adalah jenis alkohol ikatan C-4 (C4H9OH) yang terbuat dari biomassa. Penelitian ini dilakukan dengan memanfaatkan limbah kayu yang dihasilkan dari proses penggergajian kayu yang mengandung selulosa (55%), hemiselulosa (14%), dan lignin (21%). Biobutanol diproduksi dengan cara hidrolisis enzim selulase dan fermentasi bakteri Clostridium acetobutylicum. Variabel pada penelitian ini adalah penambahan enzim selulase pada proses hidrolisis (penambahan enzim atau tanpa penambahan enzim), pH awal proses fermentasi (5 atau 7) dan jumlah penambahan starter bakteri Clostridium acetobutylicum (5 atau 10 ml) dengan variasi lama proses fermentasi 2,4,6,8,10,12 hari. Parameter dalam penelitian ini adalah analisa kadar selulosa, gula tereduksi, dan kadar butanol. Berdasarkan hasil penetian, diketahui bahwa proses hidrolisis dengan penambahan enzim selulase, kondisi awal fermentasi pH 5 dan penambahan inokulum bakteri Clostridium acetobutylicum sebanyak 10 ml dengan lama waktu fermentasi 12 hari merupakan kondisi yang paling efektif menghasilkan kadar butanol tertinggi dari 50 gram limbah serbuk gergaji. Kadar butanol tertinggi sebesar 1,88 % dari 1 μL sampel hasil fermentasi yang diinjeksikan ke dalam kromatografi gas. ======================================================================== Biobutanol is a type of alcohol-bond C-4 (C4H9OH) is made from biomass. This research was carried out by utilizing sawdust generated from the sawmill which contain cellulose (55%), hemicellulose (14%), and lignin (21%). Biobutanol is produced by hydrolysis of cellulase enzymes and fermenting bacterium Clostridium acetobutylicum. Variables in this study is addition of cellulase enzymes on the hydrolysis (with or without addition of enzyme), the initial pH of fermentation processes (5 or 7) and the addition of starter bacteria Clostridium acetobutylicum (5 or 10 ml) with a time variation process of fermentation 2,4, 6, 8, 10, 12 days. The parameters in this study is cellulose analysis, sugar reduced, and levels of butanol. Based on results of this research, known that hydrolysis by addition of cellulase enzymes, initial conditions of fermentation pH 5 and addition of bacterial inoculum of 10 ml of Clostridium acetobutylicum with fermentation time of 12 days is the most effective conditions to produce the highest levels of butanol from 50 grams sawdust. The highest levels of butanol are 1,88 % from 1 μL of fermentation that injected into the gas chromatograph.

Item Type: Thesis (Undergraduate)
Additional Information: RSL 674.84 Faj p
Uncontrolled Keywords: biobutanol, Clostridium acetobutylicum, selulase, serbuk gergaji, cellulase, sawdust.
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Divisions: Faculty of Civil Engineering and Planning > Environment Engineering > (S1) Undergraduate Theses
Depositing User: Mrs Anis Wulandari
Date Deposited: 24 Aug 2017 02:33
Last Modified: 24 Aug 2017 02:33
URI: http://repository.its.ac.id/id/eprint/48746

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