Kapang Tanah Mangrove Wonorejo Pendegradasi Plastik

Kurniawati, Alfia Rahma (2018) Kapang Tanah Mangrove Wonorejo Pendegradasi Plastik. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penimbunan sampah plastik menjadi sumber polutan utama karena sifatnya yang inert. Sampah plastik sulit terdegradasi dan membutuhkan penanganan yang serius dari semua pihak. Biodegradasi merupakan solusi paling efektif untuk pengelolaan sampah plastik. Mikroorganisme yang dapat bertindak sebagai agen hayati pendegradasi plastik ini diantaranya adalah kapang. Dalam penelitian ini dilakukan isolasi kapang tanah mangrove Wonorejo yang dilanjutkan dengan uji biodegradasi dan deteksi enzim. Hasil isolasi didapatkan delapan isolat yakni Perenniporia sp. (LM1018), Trametes polyzona (LM1020), Aspergillus terreus (LM1021), Aspergillus terreus (PF1), Porostereum spadiceum (PF2), Leptosphaerulina chartarum (PF3), Aspergillus fumigatus (S2) dan Hypocreales sp. (S3), identifikasi tersebut dilakukan berdasarkan morfologi dan molekuler pada area ITS rDNA. Uji biodegradasi dilakukan selama 30 hari dengan metode pengocokan. Parameter hasil biodegradasi diantaranya adalah % efisiensi degradasi, uji FTIR, uji SEM dan AFM. Isolat A. terreus LM1021 menunjukkan potensi tertinggi dalam degradasi plastik dengan nilai % ED sebesar 12,5% pada plastik LDPE dan 4,9% pada plastik putih. Dan diikuti oleh LM1018 dengan % ED sebesar 10,6% pada plastik LDPE dan 3,4% pada plastik putih. Kerusakan plastik dari hasil biodegradasi ditunjukkan oleh hasil SEM dan AFM yang menunjukkan erosi pada permukaan plastik. Selanjutnya deteksi enzim dilakukan secara kualitatif pada medium uji lakase, mangan peroksidase, alkana hidroksilase dan lipase. Isolat LM1018 menunjukkan hasil positif pada keempat enzim, isolat LM1020 menunjukkan hasil negatif pada enzim lipase, isolat PF2 negatif pada enzim alkana hidroksilase, isolat LM1021 dan PF1 positif pada enzim lipase dan alkana hidroksilase, dan isolat S2 hanya positif pada enzim lipase. Berdasarkan hasil penelitian yang telah diperoleh diharapkan dapat menambah keragaman mikroorganisme pendegradasi plastik di Indonesia dan dapat menjadi agen bioremediasi yang potensial untuk menyelesaikan masalah limbah plastik di Indonesia. ============== Dumping plastic waste become a major source of pollutants because of its inert nature. Plastic waste is difficult to degrade and requires serious handling from all parties. Biodegradation is the most effective solution for waste management. Microorganisms that can act as biological agents such as degradation of plastic is mold. In this research, Wonorejo mangrove soil isolation was followed by biodegradation and enzyme detection test. The isolation result obtained eight isolates namely Perenniporia sp. (LM1018), Trametes polyzone (LM1020), Aspergillus terreus (LM1021), Aspergillus terreus (PF1), Porostereum spadiceum (PF2), Leptosphaerulina chartarum (PF3), Aspergillus fumigatus (S2) and Hypocreales sp. (S3), the identification is done based on morphology and molecular in ITS rDNA region. Biodegradation test was conducted for 30 days with shaking method. Parameters of biodegradation result are % degradation efficiency, FTIR test, SEM and AFM test. Isolate A. terreus LM1021 showed the highest potential in plastic degradation with an DE value of 12.5% in LDPE plastics and 4.9% in white plastic. And followed by LM1018 with% DE of 10.6% on LDPE plastic and 3.4% on white plastic. Destruction of plastic from biodegradation results is shown by SEM and AFM results indicating erosion on the surface. Further enzyme detection is carried out qualitatively on laccase test medium, manganese peroxidase, hydroxylase alkane and lipase. LM1018 showed positive results on all four enzymes, whereas LM1020 showed negative results on lipase enzyme, isolate PF2 negative in alkane hydroxylase, LM1021 and PF1 were positive on lipase and alkane hydroxylase and S2 only positive in lipase. Based on the results of the research is expected to increase the diversity of plastic degradation microorganisms in Indonesia and can be a potential bioremediation agent to solve the problem of plastic waste in Indonesia.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Isolation of molds; biodegradation tests; and enzyme detection
Subjects: Q Science > QP Physiology > QP624 Molecular biology.
Q Science > QR Microbiology
T Technology > T Technology (General)
Divisions: Faculty of Science: (S2) Biology
Depositing User: Alfia Rahma Kurniawati
Date Deposited: 26 Apr 2018 09:01
Last Modified: 26 Apr 2018 09:01
URI: http://repository.its.ac.id/id/eprint/51128

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