Karakterisasi Bakteri Brochothrix thermosphacta dan Vibrio alginolyticus serta Potensinya untuk Mereduksi Aluminium dalam Air Limbah

Kurniawan, Setyo Budi (2018) Karakterisasi Bakteri Brochothrix thermosphacta dan Vibrio alginolyticus serta Potensinya untuk Mereduksi Aluminium dalam Air Limbah. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Industri daur ulang aluminium merupakan salah satu kegiatan yang sedang berkembang di Kabupaten Jombang. Salah satu industri daur ulang aluminium berada di Desa Bakalan, Kecamatan Sumobito, Kabupaten Jombang. Kegiatan daur ulang aluminium ini menimbulkan beberapa permasalahan, seperti gangguan pernafasan, pencernaan dan iritasi ringan. Gangguan lingkungan yang dikeluhkan adalah berubahnya warna air tanah serta menurunnya kesuburan tanah di sekitar lokasi industri. Salah satu metode yang dapat digunakan untuk mereduksi pencemaran akibat logam aluminium adalah pengolahan biologis. Pengolahan biologis menggunakan isolat bakteri indigenous telah terbukti efektif dalam mengolah pencemaran akibat logam. Tujuan dari penelitian ini adalah untuk menentukan pengaruh pH terhadap proses penyisihan aluminium menggunakan dua jenis isolat bakteri kultur tunggal. Penelitain ini terdiri atas beberapa tahapan yaitu uji ketahanan asam, uji MIC (Minimum Inhibitory Concentration), uji MTC (Maximum Tolerable Concentration) serta uji bio-proses aluminium. Uji ketahanan asam dilakukan dengan metode uji OD (optical density) selama 24 jam. Uji MIC dilakukan dengan menggunakan metode penggoresan agar datar sedangkan uji MTC dilakukan dengan metode uji OD selama 24 jam. Uji bio-proses dilakukan selama 5 hari dengan menggunakan 2 jenis bakteri unggul yang didapatkan dari hasil uji ketahanan asam, MIC dan MTC. Uji bio-proses dilakukan dengan menggunakan media LB (lactose broth) yang terkontaminasi aluminium. Parameter OD, pH, suhu, jumlah koloni bakteri dan total aluminium dianalisis setiap 24jam selama waktu uji bio-proses. Enam jenis bakteri potensial didapatkan dari hasil isolasi. Spesies bakteri Vibrio alginolyticus dan Brochothrix thermosphacta terbukti memiliki ketahanan terhadap paparan aluminium dan juga suasana asam. Kedua jenis bakteri mampu bertahan pada media asam dengan pH 5. Dengan konsentrasi awal adalah 100mg/L, bakteri V. alginolyticus mampu mereduksi hingga 59,72% aluminium pada pH 5 mengikuti model pseudo orde satu, dan 50,92% pada pH 8 mengikuti model pseudo orde dua. Sementara bakteri B. thermosphacta mampu mereduksi 53,87% aluminium pada pH 5 dan 49,60% pada pH 8 mengikuti model pseudo orde dua. =============================================================================================== Aluminum recycling industry is one of the developing activities in Jombang District. One of the aluminum recycling industry is located in Bakalan, Sumobito Subdistrict, Jombang District. This aluminum recycling activity causes several issues such as respiratory, digestive and mild irritation. They are also complaining about some environmental problem such as the changing of the ground water color and the decreasing of soil fertility around the industrial site. One of the methods that can be used to reduce aluminum metal contamination is biological process. Biological process using indigenous bacterial isolates have been shown to be effective in treating metal contamination. The objective of this study was to determine the effect of pH to aluminium removal process using two types of single bacterial culture. This research consists of several stages which are acidity resistant, MIC (Minimum Inhibitory Concentration), MTC (Maximum Tolerable Concentration) and aluminium bio-process test. Acidity resistant test was carried out by using OD (Optical Density) method during 24-hour of test period. MIC test was conducted using agar streaking method and MTC test was carried out using OD method for 24-hour. Aluminium bio-process was conducted over 5 days using two types of potential bacteria that obtained from acidity resistant, MIC and MTC tests. Bio-process test was carried out by using aluminium contaminated LB (Lactose Broth) medium. OD, pH, temperature, number of bacterial colonies and total aluminium parameters were analyzed every 24-hour during the test period. Six types of potential bacteria were obtained from isolation process. Vibrio alginolyticus and Brochothrix thermosphacta species proven to have aluminium and acidity resistance. These bacteria could survive in acid medium up to pH 5. With 100mg/L of initial concentration, V. alginolyticus could reduce up to 59,72% aluminium in pH 5 following the pseudo first order reaction, and 50,92% in pH 8 following the pseudo second order reaction. B. thermosphacta could reduce 53,87% aluminium in pH 5 and 49,60% in pH 8 following the pseudo second order reaction.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Brochothrix thermosphacta; Bakteri; Bio-proses; Daur Ulang Aluminium; Jombang; Kinetik; Vibrio alginolyticus
Subjects: Q Science > QR Microbiology
T Technology > TD Environmental technology. Sanitary engineering > TD433 Water treatment plants
Divisions: Faculty of Civil Engineering and Planning > Environment Engineering > (S2) Master Theses
Depositing User: SETYO BUDI KURNIAWAN
Date Deposited: 22 Jan 2019 02:40
Last Modified: 22 Jan 2019 02:40
URI: http://repository.its.ac.id/id/eprint/51400

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