Metode Cepat Membedakan Daging Sapi Brahman Dan Babi Yorkshire Berbasis Spektrum Fluoresens

Pedulihala, Valentina (2018) Metode Cepat Membedakan Daging Sapi Brahman Dan Babi Yorkshire Berbasis Spektrum Fluoresens. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penelitian mengenai metode cepat untuk membedakan daging sapi Brahman dan babi Yorkshire telah dilakukan menggunakan spektrofotometer fluoresens. Sampel daging yang mengandung darah diekstrak menggunakan aqua DM. Hasil menunjukkan bahwa spektrum fluoresens antara sapi Brahman dan babi Yorkshire memiliki perbedaan panjang gelombang puncak spektrum fluoresens. Sapi Brahman memiliki puncak emisi pertama di panjang gelombang 340 nm dan emisi kedua 684 nm serta puncak eksitasi di panjang gelombang 342 nm. Sedangkan babi Yorkshire memiliki puncak emisi pertama di panjang gelombang 310 nm dan emisi kedua 621 nm serta puncak eksitasi di panjang gelombang 311 nm. Daging campuran ditandai dengan nilai intensitas emisi pertama dan eksitasi keluar dari range intensitas. Daging campuran dengan perbandingan babi 95% merupakan batas deteksi terkecil yang dapat dideteksi spektrofotometer fluoresen. Dalam satu kali pengukuran sampel dibutuhkan waktu 2 menit 36 detik. Berdasarkan hasil yang diperoleh, metode fluoresensi dapat mendeteksi perbedaan antara sapi Brahman dan babi Yorkshire dengan cepat, murah dan sederhana. ============== R esearch on rapid methods for differentiating meat of Brahman cattle and Yorkshire pig have been conduct ed by fluorescence spectrophotometer. The samples of muat Thar contain were dissolved in demineralized water. The results showed that fluorescence spectrum. Brahman cattles have the first emission peaks at 340 nm, second emission at 621 nm and peak excitat ion peak at 342 nm. While Yorkshire pigs have the first emission peaks at 310 nm, second emission at 621 nm and excitation peak at 311 nm. The mixture of meat had characterized in the first emission intensity value and excitatiom out of the intensity range . The mixture of meat with a 95% pig ratio is detection limit that it could detect by fluorescence spectrophotometer. Time required for measurement 2 minutes 36 second. Based on the results obtained, the fluorescence method can differentiate between Brahma n cattles and Yorkshire pigs quickly, cheaply and simply.

Item Type: Thesis (Undergraduate)
Additional Information: RSKi 535.35 Ped m
Uncontrolled Keywords: Darah, daging, sapi Brahman, babi Yorkshire, spektrofotometer fluoresens, eksitasi, emisi, Blood, meat , Brahman cattles, Yorkshire pigs, fluorescence spectrophotometer, excitation, emition
Subjects: Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions: Faculty of Science > Chemistry > (S1) Undergraduate Theses
Depositing User: Pedulihala Valentina
Date Deposited: 29 Nov 2018 04:09
Last Modified: 29 Nov 2018 06:11
URI: http://repository.its.ac.id/id/eprint/53223

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