Solikhah, Laila Kunni Marata and Erlangga, Dwi (2018) Degradasi Pati Singkong Menjadi Gula Pereduksi Melalui Proses Sonikasi Dan Hidrotermal. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
Preview |
Text
02211440000044_Undergraduate_Theses.pdf - Accepted Version Download (2MB) | Preview |
Abstract
Singkong atau ubi kayu (Manihot esculenta) mengandung karbohidrat yang memiliki kandungan terbesar berupa pati 60% (w). Pati singkong tersusun atas amilosa (15-30%) dan amilopektin (85-70%) yang terdiri dari monomer gula pereduksi. Pati dapat didegradasi menjadi gula pereduksi atau produk turunan lainnya. Metode yang sudah dikembangkan untuk proses degradasi pati salah satunya adalah metode hidrotermal dengan pre-treatment sonikasi. Tujuan dari penelitian ini adalah untuk mempelajari pengaruh kondisi operasi (amplitudo dan waktu) proses sonikasi terhadap pelarutan dan pemutusan ikatan glikosidik sebagai pre-treatment pada proses hidrotermal untuk menghasilkan gula pereduksi dengan konsentrasi lebih tinggi. Penelitian ini diawali dengan melakukan pre-treatment sonikasi pada suspensi pati (1/20(w/v)). Proses sonikasi dilakukan pada kondisi operasi suhu 35 ⁰C untuk variasi amplitudo (30% - 70%) dan variasi waktu (10-60 menit). Setelah proses pre-treatment sonikasi, produk sonikasi dilanjutkan ke proses hidrotermal pada suhu 100 oC dan tekanan 100 bar untuk berbagai waktu hidrotermal (10 - 60 menit). Hasil proses sonikasi dan hidrotermal dipisahkan antara produk cair dan padat. Produk padat dianalisis dengan Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Differential Scanning Calorymetry (DSC), Viscometer Ubbelohde dan Spektrofotometer UV-Vis dengan metode Anthrone. Sedangkan untuk produk cair dianalisa dengan metode DNS. Dari analisa diperoleh hasil, bahwa proses sonikasi cukup efektif untuk melarutkan pati dalam air dan memotong ikatan glikosidik dalam pati. Pada pre-treatment sonikasi penggunaan variasi amplitudo memberikan efek yang lebih signifikan terhadap pelarutan pati dalam air dibandingkan dengan lamanya waktu. Proses hidrotermal dengan perlakuan awal menghasilkan konsentrasi gula pereduksi yang lebih tinggi sonikasi (0,089 mg/ml) jika dibandingkan dengan proses sonikasi (0,063 mg/ml).
==========================================================================================================
Cassava (Manihot esculenta) contains carbohydrates which have greatest component of starch by 60% (w). Cassava starch is mainly composed of amylose (15-30%) and amylopectin (85-70%) consisting of glucose as monomer. Starches can be degraded into reducing sugars or others derivative products that have a high value in the food and pharmaceutical industries. One of the methods that already developed for starch degradation is hydrothermal method with sonication as pre-treatment. The purpose of this research is to study the effect of operation condition (amplitude and time) of sonication process on dissolution and termination of glycosidic bond as pre-treatment in hydrothermal process to produce higher concentration reducing sugar. This research begins with sonication as pre-treatment on starch suspension (1/20 (w/v)). The sonication process was performed on under operating conditions of 35⁰C for various amplitude (30% - 70%). After the sonication process, the product was processed in hydrothermal process with operating conditions of 100oC and 100 bar for various operating times (10 - 60 minutes). Product which obtained from the sonication and hydrothermal processes are separated between liquid and solid products. The solid product was analyzed by using Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Differential Scanning Calorimetry (DSC), Ubbelohde viscometer and Anthrone Method (for starch and cellulose). DNS (for reducing sugar) are method for analyzing the liquid product. From the study, we know that sonication process is an effective way to dissolve the starch into the water and terminate the glycosidic bond in the starch. It also known that in the pre-treatment of sonication the increase of amplitudes gives a more significant effect on the dissolution of starch into water when compared with the longer of time. When they were being compared, the hydrothermal process with pre-treatment of sonication shown higher concentration of reducing sugar produce than sonication process. Reducing sugar produced from the hydrothermal process with pre-treatment of sonication is 0,089 mg/ml, while in the process of sonication 0,063 mg/ml.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSK 572.566 82 Sol d-1 3100018076001 |
| Uncontrolled Keywords: | Pati singkong, degradasi, gula pereduksi, sonikasi, hidrotermal |
| Subjects: | T Technology > TP Chemical technology > TP1140 Polymers |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24201-(S1) Undergraduate Thesis |
| Depositing User: | Laila Kunni Marata Solikhah |
| Date Deposited: | 06 Dec 2020 21:19 |
| Last Modified: | 01 Mar 2021 03:50 |
| URI: | http://repository.its.ac.id/id/eprint/53598 |
Actions (login required)
 |
View Item |