Hardianti, Maretna Fitri and Tonica, Widya Wahyu (2018) Uji Kestabilan Kurkuminoid Dalam Natural Deep Eutetic Solvent (Nades) Secara Spektrofotometri Uv-Vis. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kurkumin dalam larutan berair mengalami reaksi degradasi hidrolitik. Stabilitas optimal kurkumin berada di bawah pH 7. Namun, di kondisi ini pH ini kelarutan kurkumin dalam media berair rendah. Pada pH > 7, molekul kurkumin sangat tidak stabil. Penelitian ini bertujuan untuk mengembangkan kurkuminoid dalam berbagai jenis Natural Deep Eutectic Solvent (NADES). NADES yang dikenal sebagai pelarut ramah lingkungan yang memiliki kemampuan tinggi untuk mengekstraksi senyawa fenolik dengan interaksi ikatan hidrogen yang terbentuk antara senyawa fenolik dan molekul NADES. Sebelumnya, kami telah menerbitkan bahwa sebelas jenis Pelarut Eutektik Alami (NADES) yang berbeda berhasil mengekstraksi kurkuminoid pada 40 ℃ selama 24 jam waktu ekstraksi; menghasilkan 0,131-0,279 mg kurkuminoids / g berat kering. Namun, hasil kurkuminoid yang diekstraksi tidak linier terhadap waktu ekstraksi. Bahkan, beberapa kurkuminoids menurun setelah diekstraksi selama 96 jam, seperti ketika MAS-H2O (1:1:18) digunakan sebagai pelarut. Membiarkan hasil yang kurang dari 59%. Oleh karena itu, uji stabilitas kurkuminoid dalam NADES diperlukan. Mengungkapkan, apakah hasil lebih rendah karena kurkuminoid terdegradasi atau membatasi dalam proses ekstraksi. Uji stabilitas kurkuminoid dilakukan pada beberapa jenis NADES yaitu CCMA-H2O (1:1:18), CCCA-H2O (1:1:18), CCGo-H2O (2:1:2), CCPro- H2O (1:1:1), CCF-H2O (5: 2: 4), dan MAG-H2O (1: 1: 18)pada 40 ℃ selama 96 jam. Metode spektrofotometer UV-Vis yang divalidasi dan sederhana diterapkan untuk mengukur kurkuminoid. Hasil uji menunjukkan kurkuminoid lebih stabil di CCMA-H2O (1:1:18), CCCA-H2O (1:1:18), dan MAG-H2O (1:1:18) dan cenderung menurun di CCGo-H2O (2:1:2), CCPro- H2O (1:1:1), dan CCF-H2O (5:2:4) dalam waktu 96 jam. Kurkuminoid itu lebih stabil pada NADES dengan kondisi asam (pH <3). Kandungan air yang lebih tinggi pada NADES dan suhu penyimpanan yang rendah (30 oC) menurunkan kestabilan kurkuminoid.
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Curcumin in aqueous solutions is exposed to hydrolytic degradative reactions. Optimum curcumin stability is below pH 7. However, in this pH region the solubility of curcumin in aqueous media is low. At pH > 7, the curcumin molecules are extremely unstable. This study aims to develop curcuminoid in different types of Natural Deep Eutectic Solvent (NADES). NADES which is known as enviromentally friendly solvent which has a high ability to extract phenolic compounds with hydrogen bonding interactions formed between phenolic compounds and NADES molecules. Previously, we have been published that eleven of different kinds of Natural Deep Eutectic Solvent (NADES) was successfully extracted kurkuminoid at 40 oC for 24 h extraction time; yielded 0.131-0.269 mg kurkuminoid /g dry weight. However, the extracted yields of kurkuminoid were not linearly to the extraction time. In fact, some of the kurkuminoid were decreased after extracted for 96 h, such when MAS-H2O (1:1:18) was used as a solvent. Leaving a yield which was approximately less than 59%. Hence, a stability test of kurkuminoid in NADES is needed. Reveals, whether the yield is lower because the curcuminoid is degraded or limiting in the extraction process. Stability test of kurkuminoid was conducted at several kinds of NADES (i.e.CCMA-H2O (1:1:18), CCCA-H2O (1:1:18), CCGo-H2O (2:1:2), CCPro-H2O (1:1:1), CCF-H2O (5:2:4), and MAG-H2O (1:1:18)) at 40 oC for 96 hours. A validated and simple method of UV-Vis spectrophotometer was applied to measure the kurkuminoid .The test results show that curcuminoid is more stable in CCMA-H2O (1:1:18), CCCA-H2O (1:1:18), and MAG-H2O (1: 1:18) and tends to decrease in CCGo-H2O (2:1:2), CCPro-H2O (1:1:1), and CCF-H2O (5:2:4) within 96 hours. The curcuminoid was more stable in NADES with acidic conditions (pH <3). Higher water content in NADES and lower storage temperatures (30°C) decreased the stability of the curcuminoid.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSK 660.284 2 Har u-1 3100018076437 |
| Uncontrolled Keywords: | Kurkumin; NADES; Spektrofotometer; Stabilitas kurkuminoid |
| Subjects: | T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions. T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24201-(S1) Undergraduate Thesis |
| Depositing User: | Hardianti Maretna Fitri |
| Date Deposited: | 27 Jul 2021 02:40 |
| Last Modified: | 27 Jul 2021 03:26 |
| URI: | http://repository.its.ac.id/id/eprint/53626 |
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