Penentuan Kadar Klorofil Daun Tanaman Sayuran Menggunakan Teknik Diffuse Reflectance Spectroscopy - Determination Of Chlorophyll Content From Vegetable Leaves Using Diffuse Reflectance Spectroscopy Technique

Prihasty, Wilda (2018) Penentuan Kadar Klorofil Daun Tanaman Sayuran Menggunakan Teknik Diffuse Reflectance Spectroscopy - Determination Of Chlorophyll Content From Vegetable Leaves Using Diffuse Reflectance Spectroscopy Technique. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Klorofil merupakan kunci utama komponen biokimia yang bertanggung jawab atas fotosintesis dan merupakan indikator kesehatan tanaman. Kadar klorofil dapat diukur secara non destruktif dan non kontak menggunakan teknik Diffuse Reflectance Spectroscopy. Phantom tersusun dari intralipid, agar, air dan klorofil dengan kadar yang telah diketahui dari teknik absorbsi, kemudian digunakan sebagai tahapan awal pengujian DRS. Fitting data spektrum reflektansi phantom dengan model matematis reflektansi difus akan digunakan untuk menentukan nilai parameter dari phantom yang dibuat. Nilai parameter tersebut yang digunakan sebagai pengukuran kadar klorofil dan akan dibandingkan dengan kadar klorofil hasil teknik absorbsi. Setelah sistem terkuantifikasi dengan baik, maka sistem akan digunakan untuk pengukuran kadar klorofil pada daun tanpa perlu mempersiapkan sampel yang rumit sebagaimana menggunakan teknik spektroskopi abasorbsi. Spektrum reflektansi phantom telah diuji dan memiliki kesamaan pola spektrum dari reflektansi daun asli. Telah dilakukan pengukuran kadar klorofil dari 3 macam sayuran dengan variasi kadar dan didapatkan hasil pengukuran kadar klorofil dengan nilai eror untuk sawi, bayam dan kangkung berturut-turut adalah 3%-10% ; 2.5%-10% dan 16%-31%. ========================================================= Chlorophyll is a main biochemistry component for photosynthesis and a helath indicator. Chlorophyll concentration can be measured by non destructive and non contact method using Diffuse Reflectance Soectroscopy. Phantom consist of intralipid, aquades, gelatin and chlorophyll with certain concentration that known by absorbtion technique is used to be a primary stage for DRS. Data fitting from phantom reflectance spectrum using diffuse reflectance mathematical model used to determine parameter from phantom. The value of parameter can be used for chlorophyll concentration measurement and will be compared with the chlorophyll concentration from absorbtion technique. When the system have been quantified well, the system will be used for chlorophyll concentration measurement for leaf without a complicated sample preparation just the way we use absorbtion technique. Phantom reflectance spectroscopy has been tested and has a similar spectrum shape with leaf’s reflectance. Chlorophyll concentration measurement has been done from three species of vegetables with three variation concentration and got error value for mustard, spinach and kale respectively 3%-10% ; 2.5%-10% dan 16%-31%.

Item Type: Thesis (Undergraduate)
Additional Information: RSF Pri p
Uncontrolled Keywords: Klorofil, Diffuse Reflectance Spectroscopy, Absorbansi, Phantom, Klorofil, Diffuse Reflectance Spectroscopy, Absorbansi, Phantom.
Subjects: Q Science > QD Chemistry > QD96F56 Fluorescence spectroscopy
Divisions: Faculty of Industrial Technology > Physics Engineering > (S1) Undergraduate Theses
Depositing User: Wilda Prihasty
Date Deposited: 28 Dec 2018 03:25
Last Modified: 28 Dec 2018 03:25
URI: http://repository.its.ac.id/id/eprint/55650

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