Proses Fermentasi Eceng Gondok Dengan Pichia Stipitis Dan Mucor Indicus Menggunakan Metode Simultaneous Saccharification Fermentation

Juwita, Nila (2015) Proses Fermentasi Eceng Gondok Dengan Pichia Stipitis Dan Mucor Indicus Menggunakan Metode Simultaneous Saccharification Fermentation. Masters thesis, Institut Technology Sepuluh Nopember.

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Abstract

Pertumbuhan eceng gondok yang pesat menyebabkan keberadaan limbah eceng gondok yang melimpah, sehingga perlu ditangani agar tidak menyebabkan pencemaran lingkungan. Salah satu cara penanganan limbah eceng gondok adalah mengubah eceng gondok menjadi bioetanol. Kandungan hemiselulosa dan selulosa yang terdapat pada eceng gondok berpotensi untuk diuraikan menjadi glukosa yang selanjutnya difermentasi sehingga menghasilkan bioetanol. Proses pembentukan bioetanol terdiri dari pretreatment, hidrolisis dan fermentasi. Pada penelitian ini proses hidrolisis dilakukan secara simultan, atau Simultaneous Saccharification and Fermentation. Pretreatment eceng gondok menggunakan P. chrysosporium yang ditambahkan kedalam reaktor berisi substrat eceng gondok dengan waktu inkubasi selama sepuluh hari. Setelah sepuluh hari substrat yang telah diinkubasi dipanaskan pada suhu 1˚0C0 selama satu jam. Proses dilanjutkan dengan menambahkan mikroorganisme untuk hidrolisis yaitu T. viride sebanyak 13,3 mL dan A. niger sebanyak 6,6 mL. Tanpa jeda waktu yang lama ditambahkan P. stipitis atau M. indicus sebanyak 10 mL serta aquadest sebanyak 90 mL, kemudian difermentasi selama empat hari. Selama empat hari masa fermentasi dilakukan pengambilan sampel pada jam ke 72 dan jam ke 96. Parameter uji adalah kadar hemiselulosa, selulosa, lignin, gula reduksi dan kadar bioetanol. Pengukuran kadar etanol menggunakan metode Gas Chromatography. iii Pada penelitian ini dihasilkan kadar etanol tertinggi pada P. stipitis sebesar 0,20 (mg/g) substrat pada reaktor dengan penambahan P. stipitis dengan konsentrasi 10% (v/v ) dengan substrat eceng gondok 10 gram. Kadar etanol terendah sebesar 0,124 (mg/g) substrat pada reaktor berisi substrat 20 g dan konsentrasi P. stipitis 5% (v/v). Kata kunci: bioetanol, eceng gondok, fermentasi, Pichia stipitis Pada reaktor dengan penambahan M. indicus kadar tertinggi sebesar 0,18 (mg/g) kondisi reaktor 10 g substrat, 10% (v/v) inokulum 72 jam fermentasi. Pada reaktor berisi 20 g substrat, konsentrasi inokulum 5% (v/v), waktu fermentasi 96 jam diperoleh kadar terendah sebesar 0,01 (mg/g ================================================================================================== The rapid growth of water hyacinth causes the presence of abundant waste water hyacinth, so that needs to be addressed so as not to cause environmental pollution. One way of handling waste water hyacinth is to convert the water hyacinth into bioethanol. The content of hemicellulose and cellulose contained in the water hyacinth has the potential to be broken down into glucose which then fermented to produce bioethanol . Bioethanol formation process consists of pretreatment, hydrolysis and fermentation. In this study, the hydrolysis process is done simultaneously, or Simultaneous Saccharification and Fermentation. Pretreatment hyacinth using P. chrysosporium were added into the reactor containing the substrate hyacinth with incubation time for ten days. After ten days that had been incubated substrate was heated at a temperature of 100˚C for one hour. The process continued by adding microorganisms to hydrolysis, namely T. viride much as 13.3 mL and A. niger as much as 6.6 mL. Without pause long time added P. stipitis or M. indicus as much as 10 mL and 90 mL of distilled water as much, then fermented for four days. During the four days of fermentation samples were taken at 72 hours and hours to 96. The test parameters the levels of hemicellulose, cellulose, lignin, reducing sugar and ethanol levels. Measurements of ethanol using Gas Chromatography. In this study the highest ethanol content was produced in P. stipitis of 0.20 (mg /g) substrate in the reactor with the addition of P. stipitis with a concentration of 10% (v/v) with 10 grams of water hyacinth substrate. Low ethanol content of 0.124 (mg / g) substrate in the reactor containing 20 g and the concentration of the substrate P. stipitis 5% (v/v). In the reactor with the addition of M. indicus highest levels of 0.18 (mg / g) 10 g substrate reactor conditions, 10% (v/v) inoculum 72 hours of fermentation. In the reactor containing 20 g of substrate, inoculum concentration of 5% (v/v), fermentation time of 96 hours obtained the lowest levels of 0.01 (mg / g )

Item Type: Thesis (Masters)
Additional Information: RTL 660.384 49 Juw p
Uncontrolled Keywords: bioetanol, eceng gondok, fermentasi, Pichia stipitis
Subjects: T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions.
Divisions: Faculty of Civil Engineering and Planning > Environment Engineering > (S2) Master Theses
Depositing User: Mr. Tondo Indra Nyata
Date Deposited: 29 Aug 2018 02:46
Last Modified: 29 Aug 2018 02:46
URI: http://repository.its.ac.id/id/eprint/59513

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