Amanah, Nadia (2015) Proses Fermentasi Eceng Gondok Oleh Zymomonas Mobilis Dan Saccharomyces Cerevisiae Dengan Metode Separate Hydrolysis And Fermentation (SHF). Masters thesis, Institut Technology Sepuluh Nopember.
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Abstract
Eceng gondok merupakan tanaman air yang mengapung di atas perairan.
Pertumbuhannya yang relatif cepat dapat menimbulkan eutrofikasi pada perairan
sehingga digolongkan sebagai gulma. Fementasi merupakan salah satu teknologi
pengolahan limbah eceng gondok yang dapat dikonversi menjadi bioetanol.
Tujuan peneliltian ini adalah mengetahui mikroorganime dan penambahan
inokulum untuk mendapatkan etanol pada kondisi optimum.
Metode fermentasi dilakukan dengan metode separate hydrolisis and
fermentation (SHF). Metode ini meliputi yahap pretreatment, hidrolisis dan
fermentasi secara terpisah. Tahap pretreatment selama 10 hari menggunakan
jamur P. chrysosporium dilanjutkan pemanasan. Tahap hidrolisis dilakukan
dengan menambahkan jamur T. viride dan A. niger 1 mL setiap 1 gram substrat,
waktu inkubasi selama 72 jam. Tahap fermentasi dengan menambahkan Z. mobilis
dan S. cerevisiae dengan konsentrasi inokulum 5% (v/v) dan 10% (v/v) pada
masing-masing reaktor. Waktu fermentasi selama 72 jam dengan pengambilan
sampel pada jam ke 48 dan ke 72. Analisis data menggunakan metode respon
surface.
Analisis kadar etanol menggunakan Gas Chromatography (GC) Hewlett
Packard (HP-series 6890). Kadar etanol tertinggi dari sampel yang telah dianalisis
diperoleh kadar etanol tertinggi adalah 1,195 mg/g dengan penambahan inokulum
S. cerevisiae 10% (v/v) pada media 10 gram eceng dan waktu fermentasi selama
48 jam.
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Water hyacinth is water plant on the water. It can grow rapidly on the
water, subsequently it creates euthropication. Moreover, the increasing of Water
Hyacinth can increase solid waste generation. However, it can be solved by
fermentation to reduce water hyacinth water generation. Fermentation is one of
water hyacinth waste treatment which converts water hyacinth to be bioethanol.
Therefore, this study focused to find optimum of microorganism and inoculum
variation condition in fermentation.
Separate hydrolysis and fermentation (SHF) was used to achive the
goals. This methode was done by three steps susch as pretreatment, hydrolisis,
and fermentation. Pretreatment was done in 10 days by using P. chrysosporium
and follow heating processed. Then, hydrolysis stage done by using T. viride and
A. niger. T. viride and A. niger were added in 1 mL for 1 g substrate, then it
incubation for 72 hours. In fermentation stage, S. cerevisiae and Z. mobilis were
added with 5% inoculum and 10% inoculum respectively. Fermentation was done
in 72 hours and sample collection was done on 48 and 72 hours. Then data were
analyszed using respon surface method.
Moreover, ethanol content was analyzed using Gas Chromatography
(GC) Hewlett Packard (HP-series 6890). Finally, the highest etanol
concentration in optimum condition is 1,195 mg/g were added S. cerevisiae 10%
in 10 g substrate and fermentation time 48 hours.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTL 660.284 49 Ama p |
| Uncontrolled Keywords: | Eceng gondok, fermentasi, S. cerevisiae, Z. mobilis |
| Subjects: | T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions. |
| Divisions: | Faculty of Civil Engineering and Planning > Environment Engineering > 25101-(S2) Master Thesis |
| Depositing User: | Mr. Tondo Indra Nyata |
| Date Deposited: | 29 Aug 2018 03:35 |
| Last Modified: | 29 Aug 2018 03:35 |
| URI: | http://repository.its.ac.id/id/eprint/59514 |
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