Proses Fermentasi Eceng Gondok Oleh Zymomonas Mobilis Dan Saccharomyces Cerevisiae Dengan Metode Separate Hydrolysis And Fermentation (SHF)

Amanah, Nadia (2015) Proses Fermentasi Eceng Gondok Oleh Zymomonas Mobilis Dan Saccharomyces Cerevisiae Dengan Metode Separate Hydrolysis And Fermentation (SHF). Masters thesis, Institut Technology Sepuluh Nopember.

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Abstract

Eceng gondok merupakan tanaman air yang mengapung di atas perairan. Pertumbuhannya yang relatif cepat dapat menimbulkan eutrofikasi pada perairan sehingga digolongkan sebagai gulma. Fementasi merupakan salah satu teknologi pengolahan limbah eceng gondok yang dapat dikonversi menjadi bioetanol. Tujuan peneliltian ini adalah mengetahui mikroorganime dan penambahan inokulum untuk mendapatkan etanol pada kondisi optimum. Metode fermentasi dilakukan dengan metode separate hydrolisis and fermentation (SHF). Metode ini meliputi yahap pretreatment, hidrolisis dan fermentasi secara terpisah. Tahap pretreatment selama 10 hari menggunakan jamur P. chrysosporium dilanjutkan pemanasan. Tahap hidrolisis dilakukan dengan menambahkan jamur T. viride dan A. niger 1 mL setiap 1 gram substrat, waktu inkubasi selama 72 jam. Tahap fermentasi dengan menambahkan Z. mobilis dan S. cerevisiae dengan konsentrasi inokulum 5% (v/v) dan 10% (v/v) pada masing-masing reaktor. Waktu fermentasi selama 72 jam dengan pengambilan sampel pada jam ke 48 dan ke 72. Analisis data menggunakan metode respon surface. Analisis kadar etanol menggunakan Gas Chromatography (GC) Hewlett Packard (HP-series 6890). Kadar etanol tertinggi dari sampel yang telah dianalisis diperoleh kadar etanol tertinggi adalah 1,195 mg/g dengan penambahan inokulum S. cerevisiae 10% (v/v) pada media 10 gram eceng dan waktu fermentasi selama 48 jam. ================================================================================================= Water hyacinth is water plant on the water. It can grow rapidly on the water, subsequently it creates euthropication. Moreover, the increasing of Water Hyacinth can increase solid waste generation. However, it can be solved by fermentation to reduce water hyacinth water generation. Fermentation is one of water hyacinth waste treatment which converts water hyacinth to be bioethanol. Therefore, this study focused to find optimum of microorganism and inoculum variation condition in fermentation. Separate hydrolysis and fermentation (SHF) was used to achive the goals. This methode was done by three steps susch as pretreatment, hydrolisis, and fermentation. Pretreatment was done in 10 days by using P. chrysosporium and follow heating processed. Then, hydrolysis stage done by using T. viride and A. niger. T. viride and A. niger were added in 1 mL for 1 g substrate, then it incubation for 72 hours. In fermentation stage, S. cerevisiae and Z. mobilis were added with 5% inoculum and 10% inoculum respectively. Fermentation was done in 72 hours and sample collection was done on 48 and 72 hours. Then data were analyszed using respon surface method. Moreover, ethanol content was analyzed using Gas Chromatography (GC) Hewlett Packard (HP-series 6890). Finally, the highest etanol concentration in optimum condition is 1,195 mg/g were added S. cerevisiae 10% in 10 g substrate and fermentation time 48 hours.

Item Type: Thesis (Masters)
Additional Information: RTL 660.284 49 Ama p
Uncontrolled Keywords: Eceng gondok, fermentasi, S. cerevisiae, Z. mobilis
Subjects: T Technology > TP Chemical technology > TP156 Crystallization. Extraction (Chemistry). Fermentation. Distillation. Emulsions.
Divisions: Faculty of Civil Engineering and Planning > Environment Engineering > (S2) Master Theses
Depositing User: Mr. Tondo Indra Nyata
Date Deposited: 29 Aug 2018 03:35
Last Modified: 29 Aug 2018 03:35
URI: http://repository.its.ac.id/id/eprint/59514

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